{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:58:23Z","timestamp":1787029103336,"version":"build-2736575974"},"reference-count":34,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2023,1,30]],"date-time":"2023-01-30T00:00:00Z","timestamp":1675036800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"EU FEDER and MCI-AEI","award":["PID2021-124554NB-I00"],"award-info":[{"award-number":["PID2021-124554NB-I00"]}]},{"name":"EU FEDER and MCI-AEI","award":["PROMETEO 2020\/078"],"award-info":[{"award-number":["PROMETEO 2020\/078"]}]},{"DOI":"10.13039\/501100003359","name":"Generalitat Valenciana","doi-asserted-by":"publisher","award":["PID2021-124554NB-I00"],"award-info":[{"award-number":["PID2021-124554NB-I00"]}],"id":[{"id":"10.13039\/501100003359","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003359","name":"Generalitat Valenciana","doi-asserted-by":"publisher","award":["PROMETEO 2020\/078"],"award-info":[{"award-number":["PROMETEO 2020\/078"]}],"id":[{"id":"10.13039\/501100003359","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>A colorimetric method for the estimation of the total content of cannabinoids in cannabis samples is proposed. The assay is based on the reaction of these compounds with the reagent Fast Blue B (FBB), which has been immobilized into polydimethylsiloxane (PDMS). The reaction and detection conditions have been established according to the results obtained for the individual cannabinoids \u03949-tetrahydrocannabidiol (THC), cannabidiol (CBD), and cannabinol (CBN), as well as for ethanolic extracts obtained from cannabis samples after ultrasonication. In contact with the extract and under basic conditions, the reagent diffuses from the PDMS device, producing a red-brown solution. The absorbances measured at 500 nm after only 1 min of exposure to the FBB\/PDMS composites led to responses proportional to the amounts of the cannabinoids in the reaction media. Those absorbances have been then transformed in total cannabinoid content using CBD as a reference compound. The potential utility of the proposed conditions has been tested by analyzing different cannabis samples. The selectivity towards other plants and drugs has been also evaluated. The present method is proposed as a simple and rapid alternative to chromatographic methods for the estimation of the total content of cannabinoids.<\/jats:p>","DOI":"10.3390\/molecules28031303","type":"journal-article","created":{"date-parts":[[2023,1,30]],"date-time":"2023-01-30T02:01:18Z","timestamp":1675044078000},"page":"1303","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["A Colorimetric Method for the Rapid Estimation of the Total Cannabinoid Content in Cannabis Samples"],"prefix":"10.3390","volume":"28","author":[{"given":"Neus","family":"Jornet-Mart\u00ednez","sequence":"first","affiliation":[{"name":"MINTOTA Research Group, Departament de Qu\u00edmica Anal\u00edtica, Facultat de Qu\u00edmica, Universitat de Val\u00e8ncia, Dr. Moliner 50, 46100 Burjassot, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josep","family":"Biosca-Mic\u00f3","sequence":"additional","affiliation":[{"name":"MINTOTA Research Group, Departament de Qu\u00edmica Anal\u00edtica, Facultat de Qu\u00edmica, Universitat de Val\u00e8ncia, Dr. Moliner 50, 46100 Burjassot, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0980-8298","authenticated-orcid":false,"given":"Pilar","family":"Camp\u00edns-Falc\u00f3","sequence":"additional","affiliation":[{"name":"MINTOTA Research Group, Departament de Qu\u00edmica Anal\u00edtica, Facultat de Qu\u00edmica, Universitat de Val\u00e8ncia, Dr. Moliner 50, 46100 Burjassot, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5106-0826","authenticated-orcid":false,"given":"Rosa","family":"Herr\u00e1ez-Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"MINTOTA Research Group, Departament de Qu\u00edmica Anal\u00edtica, Facultat de Qu\u00edmica, Universitat de Val\u00e8ncia, Dr. Moliner 50, 46100 Burjassot, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,1,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1159\/000496868","article-title":"Developing Robust Standardised Analytical Procedures for Cannabinoid Quantification: Laying the Foundations for an Emerging Cannabis-Based Pharmaceutical Industry","volume":"2","author":"Welling","year":"2019","journal-title":"Med. 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