{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T22:10:36Z","timestamp":1781820636319,"version":"3.54.5"},"reference-count":49,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2023,2,2]],"date-time":"2023-02-02T00:00:00Z","timestamp":1675296000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFC2101500"],"award-info":[{"award-number":["2021YFC2101500"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["22278216"],"award-info":[{"award-number":["22278216"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["21922806"],"award-info":[{"award-number":["21922806"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"name":"the National Natural Science Foundation of China","award":["2021YFC2101500"],"award-info":[{"award-number":["2021YFC2101500"]}]},{"name":"the National Natural Science Foundation of China","award":["22278216"],"award-info":[{"award-number":["22278216"]}]},{"name":"the National Natural Science Foundation of China","award":["21922806"],"award-info":[{"award-number":["21922806"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>The biocatalytic asymmetric reduction of prochiral ketones for the production of enantiopure alcohols is highly desirable due to its inherent advantages over chemical methods. In this study, a new bacterial strain capable of transforming ketones to corresponding alcohols with high activity and excellent enantioselectivity was discovered in a soil sample. The strain was subsequently identified as Bacillus cereus TQ-2 based on its physiological characteristics and 16S rDNA sequence analysis. Under optimized reaction conditions, the resting cells of B. cereus TQ-2 converted acetophenone to enantioenriched (R)-1-phenylethanol with 99% enantiometric excess following anti-Prelog\u2019s rule, which is scarce in biocatalytic ketone reduction. The optimum temperature for the cells was 30 \u00b0C, and considerable catalytic activity was observed over a broad pH range from 5.0 to 9.0. The cells showed enhanced catalytic activity in the presence of 15% (v\/v) glycerol as a co-substrate. The catalytic activity can also be substantially improved by adding Ca2+ or K+ ions. Moreover, the B. cereus TQ-2 cell was highly active in reducing several structurally diverse ketones and aldehydes to form corresponding alcohols with good to excellent conversion. Our study provides a versatile whole-cell biocatalyst that can be used in the asymmetric reduction of ketones for the production of chiral alcohol, thereby expanding the biocatalytic toolbox for potential practical applications.<\/jats:p>","DOI":"10.3390\/molecules28031422","type":"journal-article","created":{"date-parts":[[2023,2,2]],"date-time":"2023-02-02T03:24:49Z","timestamp":1675308289000},"page":"1422","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["New Anti-Prelog Stereospecific Whole-Cell Biocatalyst for Asymmetric Reduction of Prochiral Ketones"],"prefix":"10.3390","volume":"28","author":[{"given":"Min-Yu","family":"Wang","sequence":"first","affiliation":[{"name":"School of Pharmaceutical Sciences, Nanjing Tech University, Nanjing 211800, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shun-Ju","family":"Cai","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences, Nanjing Tech University, Nanjing 211800, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia-Chun","family":"Lin","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences, Nanjing Tech University, Nanjing 211800, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6450-0229","authenticated-orcid":false,"given":"Xiao-Jun","family":"Ji","sequence":"additional","affiliation":[{"name":"College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211800, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3768-1909","authenticated-orcid":false,"given":"Zhi-Gang","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences, Nanjing Tech University, Nanjing 211800, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"5644","DOI":"10.1002\/anie.202001876","article-title":"Biocatalytic Reduction Reactions from a Chemist\u2019s Perspective","volume":"60","author":"Hollmann","year":"2021","journal-title":"Angew. 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