{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T18:33:03Z","timestamp":1788892383725,"version":"build-2803163510"},"reference-count":61,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2023,2,3]],"date-time":"2023-02-03T00:00:00Z","timestamp":1675382400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Moringa oleifera, also called miracle tree, is a pharmaceutically important plant with a multitude of nutritional, medicinal, and therapeutic attributes. In the current study, an in-vitro-based elicitation approach was used to enhance the commercially viable bioactive compounds in an in vitro callus culture of M. oleifera. The callus culture was established and exposed to different monochromatic lights to assess the potentially interactive effects on biomass productions, biosynthesis of pharmaceutically valuable secondary metabolites, and antioxidant activity. Optimum biomass production (16.7 g\/L dry weight), total phenolic contents (TPC: 18.03 mg\/g), and flavonoid contents (TFC: 15.02 mg\/g) were recorded in callus cultures placed under continuous white light (24 h), and of other light treatments. The highest antioxidant activity, i.e., ABTS (550.69 TEAC \u00b5M) and FRAP (365.37 TEAC \u00b5M), were also noted under white light (24 h). The analysis of phytochemicals confirmed the significant impact of white light exposures on the enhanced biosynthesis of plant secondary metabolites. The enhanced levels of secondary metabolites, i.e., kaempferol (1016.04 \u00b5g\/g DW), neochlorogenic acid (998.38 \u00b5g\/g DW), quercetin (959.92 \u00b5g\/g DW), and minor compounds including luteolin, apigenin, and p-coumaric acid were observed as being highest in continuous white light (24 h with respect to the control (photoperiod). Similarly, blue light enhanced the chlorogenic acid accumulation. This study shows that differential spectral lights demonstrate a good approach for the enhancement of nutraceuticals along with novel pharmacologically important metabolites and antioxidants in the in vitro callus culture of M. oleifera.<\/jats:p>","DOI":"10.3390\/molecules28031497","type":"journal-article","created":{"date-parts":[[2023,2,6]],"date-time":"2023-02-06T01:35:13Z","timestamp":1675647313000},"page":"1497","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Effect of Wide-Spectrum Monochromatic Lights on Growth, Phytochemistry, Nutraceuticals, and Antioxidant Potential of In Vitro Callus Cultures of Moringa oleifera"],"prefix":"10.3390","volume":"28","author":[{"given":"Muhammad Naeem","family":"Bajwa","sequence":"first","affiliation":[{"name":"Department of Biotechnology, Quaid-i-Azam University, Islamabad 45320, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehnaz","family":"Khanum","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Quaid-i-Azam University, Islamabad 45320, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gouhar","family":"Zaman","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Quaid-i-Azam University, Islamabad 45320, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1501-0549","authenticated-orcid":false,"given":"Muhammad Asad","family":"Ullah","sequence":"additional","affiliation":[{"name":"School of Agriculture and Food Sciences, Gatton Campus, The University of Queensland, Gatton, QLD 4343, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Umar","family":"Farooq","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Quaid-i-Azam University, Islamabad 45320, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3850-3087","authenticated-orcid":false,"given":"Muhammad","family":"Waqas","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Quaid-i-Azam University, Islamabad 45320, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3299-3409","authenticated-orcid":false,"given":"Nisar","family":"Ahmad","sequence":"additional","affiliation":[{"name":"Center for Biotechnology and Microbiology (CB&M), University of Swat, Swat 19200, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9938-0151","authenticated-orcid":false,"given":"Christophe","family":"Hano","sequence":"additional","affiliation":[{"name":"Laboratoire de Biologie des Ligneux et des Grandes Cultures (LBLGC), University of Orleans, INRAE USC1328, F28000 Chartres, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6529-2134","authenticated-orcid":false,"given":"Bilal Haider","family":"Abbasi","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Quaid-i-Azam University, Islamabad 45320, Pakistan"},{"name":"Pakistan Academy of Sciences, Islamabad 44000, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1002\/elsc.202000078","article-title":"Recent applications of plant cell culture technology in cosmetics and foods","volume":"21","author":"Krasteva","year":"2021","journal-title":"Eng. 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