{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T10:13:23Z","timestamp":1788948803932,"version":"build-2803163510"},"reference-count":38,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2023,2,5]],"date-time":"2023-02-05T00:00:00Z","timestamp":1675555200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Bologna University"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Due to the growing presence of pesticides in the environment and in food, the concern of their impact on human health is increasing. Therefore, the development of fast and reliable detection methods is needed. Enzymatic inhibition-based biosensors represent a good alternative for replacing the more complicated and time-consuming traditional methods (chromatography, spectrophotometry, etc.). This paper describes the development of an electrochemical biosensor exploiting alkaline phosphatase as the biological recognition element and a chemically modified glassy carbon electrode as the transducer. The biosensor was prepared modifying the GCE surface by a mixture of Multi-Walled-Carbon-Nanotubes (MWCNTs) and Electrochemically-Reduced-Graphene-Oxide (ERGO) followed by the immobilization of the enzyme by cross-linking with bovine serum albumin and glutaraldehyde. The inhibition of the biosensor response caused by pesticides was established using 2-phospho-L-ascorbic acid as the enzymatic substrate, whose dephosphorylation reaction produces ascorbic acid (AA). The MWCNTs\/ERGO mixture shows a synergic effect in terms of increased sensitivity and decreased overpotential for AA oxidation. The response of the biosensor to the herbicide 2,4-dichloro-phenoxy-acetic-acid was evaluated and resulted in the concentration range 0.04\u201324 nM, with a limit of the detection of 16 pM. The determination of other pesticides was also achieved. The re-usability of the electrode was demonstrated by performing a washing procedure.<\/jats:p>","DOI":"10.3390\/molecules28041532","type":"journal-article","created":{"date-parts":[[2023,2,6]],"date-time":"2023-02-06T01:35:13Z","timestamp":1675647313000},"page":"1532","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["ALP-Based Biosensors Employing Electrodes Modified with Carbon Nanomaterials for Pesticides Detection"],"prefix":"10.3390","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8068-6679","authenticated-orcid":false,"given":"Stefano","family":"Gianvittorio","sequence":"first","affiliation":[{"name":"Department of Industrial Chemistry \u201cToso Montanari\u201d, Alma Mater Studiorum-University of Bologna, Viale del Risorgimento 4, 40136 Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6823-7501","authenticated-orcid":false,"given":"Isacco","family":"Gualandi","sequence":"additional","affiliation":[{"name":"Department of Industrial Chemistry \u201cToso Montanari\u201d, Alma Mater Studiorum-University of Bologna, Viale del Risorgimento 4, 40136 Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2844-9817","authenticated-orcid":false,"given":"Domenica","family":"Tonelli","sequence":"additional","affiliation":[{"name":"Department of Industrial Chemistry \u201cToso Montanari\u201d, Alma Mater Studiorum-University of Bologna, Viale del Risorgimento 4, 40136 Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/j.envres.2014.05.019","article-title":"Natural vs synthetic auxin: Studies on the interactions between plant hormones and biological membrane lipids","volume":"133","year":"2014","journal-title":"Environ. Res."},{"key":"ref_2","unstructured":"(2023, January 05). 2,4 D. Available online: https:\/\/www.invasive.org\/gist\/products\/handbook\/10.24-D.pdf."},{"key":"ref_3","unstructured":"Environmental Protection Agency (EPA) (2007). Reregistration Eligibility Decision for Aliphatic Alcohols Reregistration Eligibility Decision for Aliphatic Alcohols, Environmental Protection Agency (EPA)."},{"key":"ref_4","first-page":"4869","article-title":"Auxin-type herbicide drift: Effects on grapevine leaf functioning and reproductive performance","volume":"1\u20132","author":"Rossouw","year":"2021","journal-title":"IVES Tech. Rev. Vine Wine"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Peterson, M.A., Mcmaster, S.A., Riechers, D.E., Skelton, J., and Stahlman, P.W. (2016). 2,4-D Past, Present, and Future: A Review, Cambridge University Press.","DOI":"10.1614\/WT-D-15-00131.1"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1081\/CLT-100100925","article-title":"Mechanisms of Toxicity, Clinical Features, and Management of Acute Chlorophenoxy Herbicide Poisoning: A Review","volume":"38","author":"Bradberry","year":"2000","journal-title":"J. Toxicol. Clin. Toxicol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1016\/j.mrgentox.2007.03.008","article-title":"2,4-Dichlorophenoxyacetic acid: Effects on Syrian hamster embryo (SHE) cell transformation, c-Myc expression, DNA damage and apoptosis","volume":"631","author":"Maire","year":"2007","journal-title":"Mutat. Res. Genet. Toxicol. Environ. Mutagen."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Pundir, C.S., and Malik, A. (2019). Preety Bio-sensing of organophosphorus pesticides: A review. Biosens. Bioelectron., 140.","DOI":"10.1016\/j.bios.2019.111348"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1007\/BF00325738","article-title":"Comparison of amperometric and UV-spectrophotometric monitoring in the HPLC analysis of pesticides","volume":"339","author":"Yao","year":"1991","journal-title":"Fresenius. J. Anal. Chem."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/j.trac.2017.04.012","article-title":"Current scenario in organophosphates detection using electrochemical biosensors","volume":"92","author":"Kaur","year":"2017","journal-title":"TrAC-Trends Anal. Chem."},{"key":"ref_11","first-page":"1","article-title":"Recent Advances in Biosensors for Pesticide Residue Detection","volume":"10","author":"Sil","year":"2021","journal-title":"Chem. Sci. Rev. Lett."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"122356","DOI":"10.1016\/j.jclepro.2020.122356","article-title":"Recent advancements in rapid analysis of pesticides using nano biosensors: A present and future perspective","volume":"269","author":"Christopher","year":"2020","journal-title":"J. Clean. Prod."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"P\u00e9rez-Fern\u00e1ndez, B., Costa-Garc\u00eda, A., and De La Escosura- Mu\u00f1iz, A. (2020). Electrochemical (bio)sensors for pesticides detection using screen-printed electrodes. Biosensors, 10.","DOI":"10.3390\/bios10040032"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"4582","DOI":"10.1007\/s11694-021-01032-3","article-title":"Recent advances of enzyme biosensors for pesticide detection in foods","volume":"15","author":"Karadurmus","year":"2021","journal-title":"J. Food Meas. Charact."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"359","DOI":"10.3923\/ajb.2008.359.365","article-title":"Evaluation of pesticide and heavy metal toxicity using immobilized enzyme alkaline phosphatase with an electrochemical biosensor","volume":"3","author":"Shyuan","year":"2008","journal-title":"Asian J. Biochem."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.jelechem.2004.08.004","article-title":"Alkaline phosphatase inhibition based electrochemical sensors for the detection of pesticides","volume":"574","author":"Mazzei","year":"2004","journal-title":"J. Electroanal. Chem."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"3203","DOI":"10.1007\/s00216-016-9389-z","article-title":"Inhibition-based first-generation electrochemical biosensors: Theoretical aspects and application to 2,4-dichlorophenoxy acetic acid detection","volume":"408","author":"Bollella","year":"2016","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Serp, P. (2013). Carbon, Elsivier.","DOI":"10.1016\/B978-0-08-097774-4.00731-2"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Schlesinger, O., and Alfonta, L. (2018). Encapsulation of Microorganisms, Enzymes, and Redox Mediators in Graphene Oxide and Reduced Graphene Oxide, Elsevier Inc.. [1st ed.].","DOI":"10.1016\/bs.mie.2018.05.008"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"886","DOI":"10.1016\/j.bios.2016.09.102","article-title":"Nanomaterials-based enzyme electrochemical biosensors operating through inhibition for biosensing applications","volume":"89","author":"Kurbanoglu","year":"2017","journal-title":"Biosens. Bioelectron."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Zhao, G., Wang, C., Wu, Q., and Wang, Z. (2011). Analytical Methods Determination of carbamate pesticides in water and fruit samples using carbon nanotube reinforced hollow fiber liquid-phase microextraction followed by high performance liquid chromatography. Anal. Method, 1410\u20131417.","DOI":"10.1039\/c1ay05099k"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1016\/j.bios.2015.07.010","article-title":"Recent advances in biosensors based on enzyme inhibition","volume":"76","author":"Amine","year":"2016","journal-title":"Biosens. Bioelectron."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1016\/j.carbon.2021.10.027","article-title":"Surface-engineered N-doped carbon nanotubes with B-doped graphene quantum dots: Strategies to develop highly-efficient noble metal-free electrocatalyst for online-monitoring dissolved oxygen biosensor","volume":"186","author":"Wang","year":"2022","journal-title":"Carbon N. Y."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2100002","DOI":"10.1002\/smsc.202100002","article-title":"Artificial Cutaneous Sensing of Object Slippage using Soft Robotics with Closed-Loop Feedback Process","volume":"1","author":"Sekine","year":"2021","journal-title":"Small Sci."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Lee, J.H., Park, S.J., and Choi, J.W. (2019). Electrical property of graphene and its application to electrochemical biosensing. Nanomaterials, 9.","DOI":"10.3390\/nano9020297"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/j.cocis.2014.04.004","article-title":"Graphene dispersions","volume":"19","author":"Texter","year":"2014","journal-title":"Curr. Opin. Colloid Interface Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1016\/j.electacta.2017.06.071","article-title":"Electrochemical behavior of reduced graphene oxide and multi-walled carbon nanotubes composites for catechol and dopamine oxidation","volume":"246","author":"Sharma","year":"2017","journal-title":"Electrochim. Acta"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"568","DOI":"10.1016\/j.carbon.2019.11.027","article-title":"Graphene oxide in aqueous and nonaqueous media: Dispersion behaviour and solution chemistry","volume":"158","author":"Du","year":"2020","journal-title":"Carbon N. Y."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1016\/j.carbon.2022.01.056","article-title":"Electrochemically reduced graphene oxide: Preparation, composites, and applications","volume":"191","author":"Zhou","year":"2022","journal-title":"Carbon N. Y."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Feng, M., Han, H., Zhang, J., and Tachikawa, H. (2008). Electrochemical sensors based on carbon nanotubes. Electrochem. Sens. Biosens. Biomed. Appl., 459\u2013501.","DOI":"10.1016\/B978-012373738-0.50017-9"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/j.jelechem.2017.06.012","article-title":"Amperometric biosensors based on reduced GO and MWCNTs composite for polyphenols detection in fruit juices","volume":"799","author":"Vlamidis","year":"2017","journal-title":"J. Electroanal. Chem."},{"key":"ref_32","unstructured":"Britz, D. (2010). Mathematical Modeling of Biosensors, Springer."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1016\/j.biotechadv.2011.09.003","article-title":"Immobilization strategies to develop enzymatic biosensors","volume":"30","author":"Sassolas","year":"2012","journal-title":"Biotechnol. Adv."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1016\/j.bios.2018.10.014","article-title":"Origami multiple paper-based electrochemical biosensors for pesticide detection","volume":"126","author":"Arduini","year":"2019","journal-title":"Biosens. Bioelectron."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"106867","DOI":"10.1016\/j.elecom.2020.106867","article-title":"Electrochemistry Communications A mini-review: How reliable is the drop casting technique ?","volume":"121","author":"Kaliyaraj","year":"2020","journal-title":"Electrochem. Commun."},{"key":"ref_36","first-page":"462","article-title":"Electrochemical Biosensors for Detection of Pesticides and Heavy Metal Toxicants in Water: Recent Trends and Progress","volume":"1","author":"Hara","year":"2021","journal-title":"ACS Environ. Sci. Technol. Water"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"5775","DOI":"10.3390\/s8095775","article-title":"Use of Fe3O4 nanoparticles for enhancement of biosensor response to the herbicide 2,4-dichlorophenoxyacetic acid","volume":"8","author":"Loh","year":"2008","journal-title":"Sensors"},{"key":"ref_38","first-page":"299","article-title":"Biosensors based on enzyme inhibition","volume":"140","author":"Arduini","year":"2014","journal-title":"Adv. Biochem. Eng. Biotechnol."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/28\/4\/1532\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:24:41Z","timestamp":1760120681000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/28\/4\/1532"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,2,5]]},"references-count":38,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2023,2]]}},"alternative-id":["molecules28041532"],"URL":"https:\/\/doi.org\/10.3390\/molecules28041532","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,2,5]]}}}