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Cyclic AMP receptor protein (CRP) is a global regulator, and mutation of its key sites may have an important impact on E. coli virulence tolerance. Using corncob hydrolysate without ion-exchange or lime detoxification as the substrate, shake flask fermentation experiments showed that CRP mutant IS5-dG (I112L, T127G, A144T) produced 18.4 g\/L of xylitol within 34 h, and the OD600 was 9.7 at 24 h; these values were 41.5% and 21.3% higher than those of the starting strain, IS5-d, respectively. This mutant produced 82 g\/L of xylitol from corncob hydrolysate without ion-exchange or lime detoxification during fed-batch fermentation in a 15-L bioreactor, with a productivity of 1.04 g\/L\/h; these values were 173% and 174% higher than the starting strain, respectively. To our knowledge, this is the highest xylitol concentration and productivity produced by microbial fermentation using completely non-detoxified hemicellulosic hydrolysate as the substrate to date. This study also showed that alkali neutralization, high temperature sterilization, and fermentation of the hydrolysate had important effects on the xylose loss rate and xylitol production.<\/jats:p>","DOI":"10.3390\/molecules28041550","type":"journal-article","created":{"date-parts":[[2023,2,6]],"date-time":"2023-02-06T04:59:10Z","timestamp":1675659550000},"page":"1550","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Genetically Engineering Escherichia coli to Produce Xylitol from Corncob Hydrolysate without Lime Detoxification"],"prefix":"10.3390","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9751-8719","authenticated-orcid":false,"given":"Xinsong","family":"Yuan","sequence":"first","affiliation":[{"name":"School of Chemistry and Chemical Engineering, Hefei Normal University, Hefei 230601, China"},{"name":"College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiyun","family":"Cao","sequence":"additional","affiliation":[{"name":"School of Chemistry and Chemical Engineering, Hefei Normal University, Hefei 230601, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Chemistry and Chemical Engineering, Hefei Normal University, Hefei 230601, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Han","sequence":"additional","affiliation":[{"name":"School of Chemistry and Chemical Engineering, Hefei Normal University, Hefei 230601, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinmiao","family":"Zhu","sequence":"additional","affiliation":[{"name":"School of Chemistry and Chemical Engineering, Hefei Normal University, Hefei 230601, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianping","family":"Lin","sequence":"additional","affiliation":[{"name":"College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China"},{"name":"Ningbo Research Institute, Zhejiang University, Ningbo 315100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lirong","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6962-9702","authenticated-orcid":false,"given":"Mianbin","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China"},{"name":"Ningbo Research Institute, Zhejiang University, Ningbo 315100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"642","DOI":"10.1016\/j.biortech.2014.10.150","article-title":"Improving xylitol production at elevated temperature with engineered Kluyveromyces marxianus through over-expressing transporters","volume":"175","author":"Zhang","year":"2015","journal-title":"Bioresour. 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