{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T03:50:53Z","timestamp":1785210653458,"version":"3.55.0"},"reference-count":40,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2023,2,16]],"date-time":"2023-02-16T00:00:00Z","timestamp":1676505600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"China Postdoctoral Science Foundation","award":["2021M690075"],"award-info":[{"award-number":["2021M690075"]}]},{"name":"China Postdoctoral Science Foundation","award":["2021T140308"],"award-info":[{"award-number":["2021T140308"]}]},{"name":"China Postdoctoral Science Foundation","award":["2021K193B"],"award-info":[{"award-number":["2021K193B"]}]},{"name":"Postdoctoral Research Funding Program of Jiangsu Province","award":["2021M690075"],"award-info":[{"award-number":["2021M690075"]}]},{"name":"Postdoctoral Research Funding Program of Jiangsu Province","award":["2021T140308"],"award-info":[{"award-number":["2021T140308"]}]},{"name":"Postdoctoral Research Funding Program of Jiangsu Province","award":["2021K193B"],"award-info":[{"award-number":["2021K193B"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Accurate detection of H2S is crucial to understanding the occurrence and development of H2S-related diseases. However, the accurate and sensitive detection of H2S in vivo still faces great challenges due to the characteristics of H2S diffusion and short half-life. Herein, we report a H2S-activatable ratiometric near-infrared (NIR) fluorescence liposome nanoprobe HS-CG by the thin-film hydration method. HS-CG shows \u201calways on\u201d fluorescence signal at 816 nm and low fluorescence signal at 728 nm; the NIR fluorescence ratio between 728 and 816 nm (F728\/F816) is low. Upon reaction with H2S, the fluorescence at 728 nm could be more rapidly turned on due to strong electrostatic interaction between enriched HS\u2212 and positively charged 1,2-dihexadecanoyl-sn-glycero-3-phosphocholine (DPPC) doped in the liposome nanoprobe HS-CG, resulting in a large enhancement of F728\/F816, which allows for sensitive visualization of the tumor H2S levels in vivo. This study demonstrates that this strategy of electrostatic adsorption between HS\u2212 and positively charged molecules provides a new way to enhance the reaction rate of the probe and H2S, thus serving as an effective platform for improving the sensitivity of imaging.<\/jats:p>","DOI":"10.3390\/molecules28041898","type":"journal-article","created":{"date-parts":[[2023,2,17]],"date-time":"2023-02-17T01:32:56Z","timestamp":1676597576000},"page":"1898","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Ratiometric Near-Infrared Fluorescence Liposome Nanoprobe for H2S Detection In Vivo"],"prefix":"10.3390","volume":"28","author":[{"given":"Luyan","family":"Wu","sequence":"first","affiliation":[{"name":"Jiangsu Key Laboratory for Biosensors, Key Laboratory for Organic Electronics and Information Displays, Institute of Advanced Materials (IAM), Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing University of Posts and Telecommunications, Nanjing 210023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yili","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Analytical Chemistry for Life Science, Chemistry and Biomedicine Innovation Center (ChemBIC), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210033, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junya","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Analytical Chemistry for Life Science, Chemistry and Biomedicine Innovation Center (ChemBIC), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210033, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yinxing","family":"Miao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Analytical Chemistry for Life Science, Chemistry and Biomedicine Innovation Center (ChemBIC), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210033, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruibing","family":"An","sequence":"additional","affiliation":[{"name":"Institute of Optical Functional Materials for Biomedical Imaging, School of Chemistry and Pharmaceutical Engineering, Shandong First Medical University & Shandong Academy of Medical Science, Taian 271016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"10216","DOI":"10.1021\/jacs.5b04196","article-title":"A bright fluorescent probe for H2S enables analyte-responsive, 3D imaging in live zebrafish using light sheet fluorescence microscopy","volume":"137","author":"Hammers","year":"2015","journal-title":"J. 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Int."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/28\/4\/1898\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:38:36Z","timestamp":1760121516000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/28\/4\/1898"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,2,16]]},"references-count":40,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2023,2]]}},"alternative-id":["molecules28041898"],"URL":"https:\/\/doi.org\/10.3390\/molecules28041898","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,2,16]]}}}