{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:54:19Z","timestamp":1760147659464,"version":"build-2065373602"},"reference-count":26,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2023,2,20]],"date-time":"2023-02-20T00:00:00Z","timestamp":1676851200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82104284","20JCQNJC00320","2018YFC1708203","2021-I2M-1-042","2022-I2M-2-002","2018PT35031"],"award-info":[{"award-number":["82104284","20JCQNJC00320","2018YFC1708203","2021-I2M-1-042","2022-I2M-2-002","2018PT35031"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Natural Science Foundation of Tianjin","award":["82104284","20JCQNJC00320","2018YFC1708203","2021-I2M-1-042","2022-I2M-2-002","2018PT35031"],"award-info":[{"award-number":["82104284","20JCQNJC00320","2018YFC1708203","2021-I2M-1-042","2022-I2M-2-002","2018PT35031"]}]},{"name":"National Key R&amp;D Program of China","award":["82104284","20JCQNJC00320","2018YFC1708203","2021-I2M-1-042","2022-I2M-2-002","2018PT35031"],"award-info":[{"award-number":["82104284","20JCQNJC00320","2018YFC1708203","2021-I2M-1-042","2022-I2M-2-002","2018PT35031"]}]},{"name":"CAMS Innovation Fund for Medical Sciences","award":["82104284","20JCQNJC00320","2018YFC1708203","2021-I2M-1-042","2022-I2M-2-002","2018PT35031"],"award-info":[{"award-number":["82104284","20JCQNJC00320","2018YFC1708203","2021-I2M-1-042","2022-I2M-2-002","2018PT35031"]}]},{"name":"Non-profit Central Research Institute Fund of Chinese Academy of Medical Sciences","award":["82104284","20JCQNJC00320","2018YFC1708203","2021-I2M-1-042","2022-I2M-2-002","2018PT35031"],"award-info":[{"award-number":["82104284","20JCQNJC00320","2018YFC1708203","2021-I2M-1-042","2022-I2M-2-002","2018PT35031"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Bentysrepinine (Y101) is a novel phenylalanine dipeptide for the treatment of hepatitis B virus. Renal excretion played an important role in the elimination of Y101 and its metabolites, M8 and M9, in healthy Chinese subjects, although the molecular mechanisms of renal excretion and potential drug\u2013drug interactions (DDIs) remain unclear. The present study aimed to determine the organic anion transporters (OATs) involved in the renal disposition of Y101 and to predict the potential DDI between Y101 and entecavir, the first-line agent against HBV and a substrate of OAT1\/3. Pharmacokinetic studies and uptake assays using rat kidney slices, as well as hOAT1\/3-HEK293 cells, were performed to evaluate potential DDI. The co-administration of probenecid (an inhibitor of OATs) significantly increased the plasma concentrations and area under the plasma concentration\u2013time curves of M8 and M9 but not Y101, while reduced renal clearance and the cumulative urinary excretion of M8 were observed in rats. The time course of Y101 and M8 uptake via rat kidney slices was temperature-dependent. Moreover, the uptake of M8 was inhibited significantly by probenecid and benzylpenicillin, but not by p-aminohippurate or tetraethyl ammonium. M8 was found to be a substrate of hOAT3, but Y101 is not a substrate of either hOAT1 or hOAT3. Additionally, the entecavir inhibited the uptake of M8 in the hOAT3-transfected cells and rat kidney slices in vitro. Interestingly, no significant changes were observed in the pharmacokinetic parameters of Y101, M8 or entecavir, regardless of intravenous or oral co-administration of Y101 and entecavir in rats. In conclusion, M8 is a substrate of OAT3 in rats and humans. Furthermore, M8 also mediates the DDI between Y101 and entecavir in vitro, mediated by OAT3. We speculate that it would be safe to use Y101 with entecavir in clinical practice. Our results provide useful information with which to predict the DDIs between Y101 and other drugs that act as substrates of OAT3.<\/jats:p>","DOI":"10.3390\/molecules28041995","type":"journal-article","created":{"date-parts":[[2023,2,21]],"date-time":"2023-02-21T01:39:28Z","timestamp":1676943568000},"page":"1995","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["OAT3 Participates in Drug\u2013Drug Interaction between Bentysrepinine and Entecavir through Interactions with M8\u2014A Metabolite of Bentysrepinine\u2014In Rats and Humans In Vitro"],"prefix":"10.3390","volume":"28","author":[{"given":"Aijie","family":"Zhang","sequence":"first","affiliation":[{"name":"Wuya College of Innovation, Shenyang Pharmaceutical University, Shenyang 110016, China"},{"name":"State Key Laboratory of Functions and Applications of Medicinal Plants, Key Laboratory of Pharmaceutics of Guizhou Province, Guizhou Medical University, Guiyang 550025, China"},{"name":"Key Laboratory of Radiopharmacokinetics for Innovative Drugs, Chinese Academy of Medical Sciences, and Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fanlong","family":"Yang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Radiopharmacokinetics for Innovative Drugs, Chinese Academy of Medical Sciences, and Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Yuan","sequence":"additional","affiliation":[{"name":"School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cai","family":"Li","sequence":"additional","affiliation":[{"name":"School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4056-7987","authenticated-orcid":false,"given":"Xiaokui","family":"Huo","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Dalian Medical University, Dalian 116044, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Liu","sequence":"additional","affiliation":[{"name":"Heping District Center for Disease Control and Prevention, Tianjin 300070, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shenzhi","family":"Zhou","sequence":"additional","affiliation":[{"name":"Wuya College of Innovation, Shenyang Pharmaceutical University, Shenyang 110016, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Li","sequence":"additional","affiliation":[{"name":"Wuya College of Innovation, Shenyang Pharmaceutical University, Shenyang 110016, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Na","family":"Zhang","sequence":"additional","affiliation":[{"name":"Wuya College of Innovation, Shenyang Pharmaceutical University, Shenyang 110016, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianfeng","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Radiopharmacokinetics for Innovative Drugs, Chinese Academy of Medical Sciences, and Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shiqi","family":"Dong","sequence":"additional","affiliation":[{"name":"Key Laboratory of Radiopharmacokinetics for Innovative Drugs, Chinese Academy of Medical Sciences, and Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huirong","family":"Fan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Radiopharmacokinetics for Innovative Drugs, Chinese Academy of Medical Sciences, and Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Peng","sequence":"additional","affiliation":[{"name":"Wuya College of Innovation, Shenyang Pharmaceutical University, Shenyang 110016, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiang","family":"Zheng","sequence":"additional","affiliation":[{"name":"Wuya College of Innovation, Shenyang Pharmaceutical University, Shenyang 110016, China"},{"name":"State Key Laboratory of Functions and Applications of Medicinal Plants, Key Laboratory of Pharmaceutics of Guizhou Province, Guizhou Medical University, Guiyang 550025, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1007\/s40261-020-00909-3","article-title":"Phase I, first-in-human, single and multiple ascending dose- and food-effect studies to assess the safety, tolerability and pharmacokinetics of a novel anti-hepatitis B virus drug, bentysrepinine (Y101), in healthy Chinese subjects","volume":"40","author":"Liu","year":"2020","journal-title":"Clin. 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