{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T01:18:00Z","timestamp":1789003080349,"version":"build-2803163510"},"reference-count":42,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2023,2,24]],"date-time":"2023-02-24T00:00:00Z","timestamp":1677196800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the camellia research project of Jiangxi Forestry Bureau","award":["JXYCZX(2023)030201"],"award-info":[{"award-number":["JXYCZX(2023)030201"]}]},{"name":"the camellia research project of Jiangxi Forestry Bureau","award":["20203ABC28W016"],"award-info":[{"award-number":["20203ABC28W016"]}]},{"name":"the camellia research project of Jiangxi Forestry Bureau","award":["20192BBFL60014"],"award-info":[{"award-number":["20192BBFL60014"]}]},{"name":"the camellia research project of Jiangxi Forestry Bureau","award":["20212BAB215012"],"award-info":[{"award-number":["20212BAB215012"]}]},{"name":"the camellia research project of Jiangxi Forestry Bureau","award":["2020CXZX07"],"award-info":[{"award-number":["2020CXZX07"]}]},{"name":"Jiangxi Province Major Science and Technology Research and Development Project","award":["JXYCZX(2023)030201"],"award-info":[{"award-number":["JXYCZX(2023)030201"]}]},{"name":"Jiangxi Province Major Science and Technology Research and Development Project","award":["20203ABC28W016"],"award-info":[{"award-number":["20203ABC28W016"]}]},{"name":"Jiangxi Province Major Science and Technology Research and Development Project","award":["20192BBFL60014"],"award-info":[{"award-number":["20192BBFL60014"]}]},{"name":"Jiangxi Province Major Science and Technology Research and Development Project","award":["20212BAB215012"],"award-info":[{"award-number":["20212BAB215012"]}]},{"name":"Jiangxi Province Major Science and Technology Research and Development Project","award":["2020CXZX07"],"award-info":[{"award-number":["2020CXZX07"]}]},{"name":"the Key Projects of Key R&amp;D Program of Jiangxi Province","award":["JXYCZX(2023)030201"],"award-info":[{"award-number":["JXYCZX(2023)030201"]}]},{"name":"the Key Projects of Key R&amp;D Program of Jiangxi Province","award":["20203ABC28W016"],"award-info":[{"award-number":["20203ABC28W016"]}]},{"name":"the Key Projects of Key R&amp;D Program of Jiangxi Province","award":["20192BBFL60014"],"award-info":[{"award-number":["20192BBFL60014"]}]},{"name":"the Key Projects of Key R&amp;D Program of Jiangxi Province","award":["20212BAB215012"],"award-info":[{"award-number":["20212BAB215012"]}]},{"name":"the Key Projects of Key R&amp;D Program of Jiangxi Province","award":["2020CXZX07"],"award-info":[{"award-number":["2020CXZX07"]}]},{"name":"Natural Science Foundation of Jiangxi Province","award":["JXYCZX(2023)030201"],"award-info":[{"award-number":["JXYCZX(2023)030201"]}]},{"name":"Natural Science Foundation of Jiangxi Province","award":["20203ABC28W016"],"award-info":[{"award-number":["20203ABC28W016"]}]},{"name":"Natural Science Foundation of Jiangxi Province","award":["20192BBFL60014"],"award-info":[{"award-number":["20192BBFL60014"]}]},{"name":"Natural Science Foundation of Jiangxi Province","award":["20212BAB215012"],"award-info":[{"award-number":["20212BAB215012"]}]},{"name":"Natural Science Foundation of Jiangxi Province","award":["2020CXZX07"],"award-info":[{"award-number":["2020CXZX07"]}]},{"name":"Special Research Project on Camphor Tree (KRPCT) of Jiangxi Forestry Department","award":["JXYCZX(2023)030201"],"award-info":[{"award-number":["JXYCZX(2023)030201"]}]},{"name":"Special Research Project on Camphor Tree (KRPCT) of Jiangxi Forestry Department","award":["20203ABC28W016"],"award-info":[{"award-number":["20203ABC28W016"]}]},{"name":"Special Research Project on Camphor Tree (KRPCT) of Jiangxi Forestry Department","award":["20192BBFL60014"],"award-info":[{"award-number":["20192BBFL60014"]}]},{"name":"Special Research Project on Camphor Tree (KRPCT) of Jiangxi Forestry Department","award":["20212BAB215012"],"award-info":[{"award-number":["20212BAB215012"]}]},{"name":"Special Research Project on Camphor Tree (KRPCT) of Jiangxi Forestry Department","award":["2020CXZX07"],"award-info":[{"award-number":["2020CXZX07"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>At present, the technology used for the extraction and purification of Camellia oleifera saponins generally has the problems of high cost and low purity, and the quantitative detection of Camellia oleifera saponins also has the problems of low sensitivity and easy interference from impurities. To solve these problems, this paper aimed to use liquid chromatography for the quantitative detection of Camellia oleifera saponins, and to adjust and optimize the related conditions. In our study, the average recovery of Camellia oleifera saponins obtained was 100.42%. The RSD of precision test was 0.41%. The RSD of the repeatability test was 0.22%. The detection limit of the liquid chromatography was 0.06 mg\/L, and the quantification limit was 0.2 mg\/L. In order to improve the yield and purity, the Camellia oleifera saponins were extracted from Camellia oleifera Abel. seed meal by methanol extraction. Then, the extracted Camellia oleifera saponins were extracted with an ammonium sulfate\/propanol aqueous two-phase system. We optimized the purification process of formaldehyde extraction and aqueous two-phase extraction. Under the optimal purification process, the purity of Camellia oleifera saponins extracted by methanol was 36.15%, and the yield was 25.24%. The purity of Camellia oleifera saponins obtained by aqueous two-phase extraction was 83.72%. Thus, this study can provide a reference standard for rapid and efficient detection and analysis of Camellia oleifera saponins for industrial extraction and purification.<\/jats:p>","DOI":"10.3390\/molecules28052132","type":"journal-article","created":{"date-parts":[[2023,2,27]],"date-time":"2023-02-27T02:10:46Z","timestamp":1677463846000},"page":"2132","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Quantitative Analysis of Camellia oleifera Seed Saponins and Aqueous Two-Phase Extraction and Separation"],"prefix":"10.3390","volume":"28","author":[{"given":"Lifang","family":"Zhu","sequence":"first","affiliation":[{"name":"National Forestry and Grassland Bureau Woody Spice (East China) Engineering Technology Research Center, the Institute of Plant Natural Products and Forest Products Chemical Engineering, College of Forestry, Jiangxi Agricultural University, Nanchang 330045, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanshan","family":"Wang","sequence":"additional","affiliation":[{"name":"National Forestry and Grassland Bureau Woody Spice (East China) Engineering Technology Research Center, the Institute of Plant Natural Products and Forest Products Chemical Engineering, College of Forestry, Jiangxi Agricultural University, Nanchang 330045, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Faling","family":"Wan","sequence":"additional","affiliation":[{"name":"National Forestry and Grassland Bureau Woody Spice (East China) Engineering Technology Research Center, the Institute of Plant Natural Products and Forest Products Chemical Engineering, College of Forestry, Jiangxi Agricultural University, Nanchang 330045, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yihong","family":"Zhou","sequence":"additional","affiliation":[{"name":"National Forestry and Grassland Bureau Woody Spice (East 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Engineering, College of Forestry, Jiangxi Agricultural University, Nanchang 330045, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Wang","sequence":"additional","affiliation":[{"name":"National Forestry and Grassland Bureau Woody Spice (East China) Engineering Technology Research Center, the Institute of Plant Natural Products and Forest Products Chemical Engineering, College of Forestry, Jiangxi Agricultural University, Nanchang 330045, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hai","family":"Luo","sequence":"additional","affiliation":[{"name":"National Forestry and Grassland Bureau Woody Spice (East China) Engineering Technology Research Center, the Institute of Plant Natural Products and Forest Products Chemical Engineering, College of Forestry, Jiangxi Agricultural University, Nanchang 330045, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1622-563X","authenticated-orcid":false,"given":"Shengliang","family":"Liao","sequence":"additional","affiliation":[{"name":"National Forestry and Grassland Bureau Woody Spice (East China) Engineering Technology Research Center, the Institute of Plant Natural Products and Forest Products Chemical Engineering, College of Forestry, Jiangxi Agricultural University, Nanchang 330045, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"He","sequence":"additional","affiliation":[{"name":"National Forestry and Grassland Bureau Woody Spice (East China) Engineering Technology Research Center, the Institute of Plant Natural Products and Forest Products Chemical Engineering, College of Forestry, Jiangxi Agricultural University, Nanchang 330045, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuling","family":"Yang","sequence":"additional","affiliation":[{"name":"National Forestry and Grassland Bureau Woody Spice (East China) Engineering Technology Research Center, the Institute of Plant Natural Products and Forest Products Chemical Engineering, College of Forestry, Jiangxi Agricultural University, Nanchang 330045, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang","family":"Li","sequence":"additional","affiliation":[{"name":"National Forestry and Grassland Bureau Woody Spice (East China) Engineering Technology Research Center, the Institute of Plant Natural Products and Forest Products Chemical Engineering, College of Forestry, Jiangxi Agricultural University, Nanchang 330045, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiuxiu","family":"Zou","sequence":"additional","affiliation":[{"name":"National Forestry and Grassland Bureau Woody Spice (East 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