{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T04:25:47Z","timestamp":1767846347234,"version":"3.49.0"},"reference-count":43,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2023,2,27]],"date-time":"2023-02-27T00:00:00Z","timestamp":1677456000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China","award":["31771941"],"award-info":[{"award-number":["31771941"]}]},{"name":"National Natural Science Foundation of China","award":["222102110040"],"award-info":[{"award-number":["222102110040"]}]},{"name":"National Natural Science Foundation of China","award":["21A550009"],"award-info":[{"award-number":["21A550009"]}]},{"name":"National Natural Science Foundation of China","award":["22A550007"],"award-info":[{"award-number":["22A550007"]}]},{"name":"National Natural Science Foundation of China","award":["GG2021022"],"award-info":[{"award-number":["GG2021022"]}]},{"name":"Science and Technology Key Project of Henan province","award":["31771941"],"award-info":[{"award-number":["31771941"]}]},{"name":"Science and Technology Key Project of Henan province","award":["222102110040"],"award-info":[{"award-number":["222102110040"]}]},{"name":"Science and Technology Key Project of Henan province","award":["21A550009"],"award-info":[{"award-number":["21A550009"]}]},{"name":"Science and Technology Key Project of Henan province","award":["22A550007"],"award-info":[{"award-number":["22A550007"]}]},{"name":"Science and Technology Key Project of Henan province","award":["GG2021022"],"award-info":[{"award-number":["GG2021022"]}]},{"name":"Key Scientific Research Projects of Universities in Henan Province","award":["31771941"],"award-info":[{"award-number":["31771941"]}]},{"name":"Key Scientific Research Projects of Universities in Henan Province","award":["222102110040"],"award-info":[{"award-number":["222102110040"]}]},{"name":"Key Scientific Research Projects of Universities in Henan Province","award":["21A550009"],"award-info":[{"award-number":["21A550009"]}]},{"name":"Key Scientific Research Projects of Universities in Henan Province","award":["22A550007"],"award-info":[{"award-number":["22A550007"]}]},{"name":"Key Scientific Research Projects of Universities in Henan Province","award":["GG2021022"],"award-info":[{"award-number":["GG2021022"]}]},{"name":"Science and Technology Key Project of Xinxiang city","award":["31771941"],"award-info":[{"award-number":["31771941"]}]},{"name":"Science and Technology Key Project of Xinxiang city","award":["222102110040"],"award-info":[{"award-number":["222102110040"]}]},{"name":"Science and Technology Key Project of Xinxiang city","award":["21A550009"],"award-info":[{"award-number":["21A550009"]}]},{"name":"Science and Technology Key Project of Xinxiang city","award":["22A550007"],"award-info":[{"award-number":["22A550007"]}]},{"name":"Science and Technology Key Project of Xinxiang city","award":["GG2021022"],"award-info":[{"award-number":["GG2021022"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>In this study, sweet potato \u03b2-amylase (SPA) was modified by methoxy polyethylene glycol maleimide (molecular weight 5000, Mal-mPEG5000) to obtain the Mal-mPEG5000-SPA modified \u03b2-amylase and the interaction mechanism between SPA and Mal-mPEG5000 was investigated. the changes in the functional groups of different amide bands and modifications in the secondary structure of enzyme protein were analyzed using infrared spectroscopy and circular dichroism spectroscopy. The addition of Mal-mPEG5000 transformed the random curl in the SPA secondary structure into a helix structure, forming a folded structure. The Mal-mPEG5000 improved the thermal stability of SPA and protected the structure of the protein from breaking by the surrounding. The thermodynamic analysis further implied that the intermolecular forces between SPA and Mal-mPEG5000 were hydrophobic interactions and hydrogen bonds due to the positive values of \u0394H\u03b8 and \u0394S\u03b8. Furthermore, the calorie titration data showed that the binding stoichiometry for the complexation of Mal-mPEG5000 to SPA was 1.26, and the binding constant was 1.256 \u00d7 107 mol\/L. The binding reaction resulted from negative enthalpy, indicating that the interaction of SPA and Mal-mPEG5000 was induced by the van der Waals force and hydrogen bonding. The UV results showed the formation of non-luminescent material during the interaction, the Fluorescence results confirmed that the mechanism between SPA and Mal-mPEG5000 was static quenching. According to the fluorescence quenching measurement, the binding constant (KA) values were 4.65 \u00d7 104 L\u00b7mol\u22121 (298K), 5.56 \u00d7 104 L\u00b7mol\u22121 (308K), and 6.91 \u00d7 104 L\u00b7mol\u22121 (318K), respectively.<\/jats:p>","DOI":"10.3390\/molecules28052188","type":"journal-article","created":{"date-parts":[[2023,2,27]],"date-time":"2023-02-27T02:10:46Z","timestamp":1677463846000},"page":"2188","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Study on the Interaction Mechanism of Methoxy Polyethylene Glycol Maleimide with Sweet Potato \u03b2-Amylase"],"prefix":"10.3390","volume":"28","author":[{"given":"Xinhong","family":"Liang","sequence":"first","affiliation":[{"name":"Henan Institute of Science and Technology, School of Food Science, Xinxiang 453003, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3667-2151","authenticated-orcid":false,"given":"Yaxin","family":"Kong","sequence":"additional","affiliation":[{"name":"Henan Institute of Science and Technology, School of Food Science, Xinxiang 453003, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huadi","family":"Sun","sequence":"additional","affiliation":[{"name":"Xinxiang Institute of Engineering, School of Food Engineering, Xinxiang 453003, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruixiang","family":"Zhao","sequence":"additional","affiliation":[{"name":"Henan Institute of Science and Technology, School of Food Science, Xinxiang 453003, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lingxia","family":"Jiao","sequence":"additional","affiliation":[{"name":"Henan Institute of Science and Technology, School of Food Science, Xinxiang 453003, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7703-698X","authenticated-orcid":false,"given":"Wanli","family":"Zhang","sequence":"additional","affiliation":[{"name":"Henan Institute of Science and Technology, School of Food Science, Xinxiang 453003, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bing","family":"Liu","sequence":"additional","affiliation":[{"name":"Xinxiang Institute of Engineering, School of Food Engineering, Xinxiang 453003, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"106722","DOI":"10.1016\/j.foodhyd.2021.106722","article-title":"The structural and functional properties of corn starch treated with endogenous malt amylases","volume":"117","author":"Gui","year":"2021","journal-title":"Food Hydrocoll."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1002\/j.2050-0416.1985.tb04300.x","article-title":"Beta-amylase sulphydryl and disulphide group reactions: Additional aspects on enzyme inhibition by ascorbic acid","volume":"91","author":"Baker","year":"1985","journal-title":"J. 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