{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,10]],"date-time":"2026-05-10T16:27:36Z","timestamp":1778430456701,"version":"3.51.4"},"reference-count":37,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2023,3,7]],"date-time":"2023-03-07T00:00:00Z","timestamp":1678147200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000780","name":"European Union","doi-asserted-by":"publisher","award":["KK.01.1.1.04.0002"],"award-info":[{"award-number":["KK.01.1.1.04.0002"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"European Union","doi-asserted-by":"publisher","award":["DOK-2021-02-1355"],"award-info":[{"award-number":["DOK-2021-02-1355"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Croatian Science Foundation","award":["KK.01.1.1.04.0002"],"award-info":[{"award-number":["KK.01.1.1.04.0002"]}]},{"name":"Croatian Science Foundation","award":["DOK-2021-02-1355"],"award-info":[{"award-number":["DOK-2021-02-1355"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>The objective of this study was to evaluate the release rate, duration, and biological efficiency of yeast volatile compounds associated with olive fruit flies in slow-release dispensers, polypropylene vials, and rubber septa attached to yellow sticky traps under different environmental conditions in order to protect the environment, humans, and nontarget organisms. Isoamyl alcohol, 2-octanone, and 2-phenethyl acetate were placed in dispensers and tested over a four-week experiment. The weight loss of the volatile compounds in both dispensers was measured, and a rapid, inexpensive, and simple HS-GC\/FID method was developed to determine the residual amount of volatiles in the septa. 2-Phenethyl acetate stood out in the rubber septa and showed a statistically significant difference in the release ratio compared to the other volatiles under all conditions tested. Our results showed that the attraction of olive fruit flies increased with decreasing concentrations of the tested volatiles. Regarding the number of flies attracted by rubber septa containing 2-phenethyl acetate, significantly better results were obtained than for septa containing isoamyl alcohol and 2-octanone, in contrast to the attraction of olive fruit flies to polypropylene vials containing these compounds but without significant difference. Since the presence of all tested chemicals was detected during the experiment, this opens the possibility of using more environmentally friendly and cost-effective dispensers with a significantly lower amount of semiochemicals.<\/jats:p>","DOI":"10.3390\/molecules28062431","type":"journal-article","created":{"date-parts":[[2023,3,8]],"date-time":"2023-03-08T02:08:14Z","timestamp":1678241294000},"page":"2431","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["A Lifetime of a Dispenser-Release Rates of Olive Fruit Fly-Associated Yeast Volatile Compounds and Their Influence on Olive Fruit Fly (Bactrocera oleae Rossi) Attraction"],"prefix":"10.3390","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8654-4740","authenticated-orcid":false,"given":"Maja","family":"Ver\u0161i\u0107 Bratin\u010devi\u0107","sequence":"first","affiliation":[{"name":"Department of Applied Science, Institute for Adriatic Crops and Karst Reclamation, Put Duilova 11, 21000 Split, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana","family":"Bego","sequence":"additional","affiliation":[{"name":"Department of Applied Science, Institute for Adriatic Crops and Karst Reclamation, Put Duilova 11, 21000 Split, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivana","family":"Ni\u017eeti\u0107 Kosovi\u0107","sequence":"additional","affiliation":[{"name":"Ericsson Nikola Tesla, Polji\u010dka Cesta 39, 21000 Split, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8834-518X","authenticated-orcid":false,"given":"Maja","family":"Juki\u0107 \u0160pika","sequence":"additional","affiliation":[{"name":"Department of Applied Science, Institute for Adriatic Crops and Karst Reclamation, Put Duilova 11, 21000 Split, Croatia"},{"name":"Center of Excellence for Biodiversity and Molecular Plant Breeding (CoE CroP-BioDiv), Sveto\u0161imunska Cesta 25, 10000 Zagreb, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1871-969X","authenticated-orcid":false,"given":"Filipa","family":"Burul","sequence":"additional","affiliation":[{"name":"Department of Applied Science, Institute for Adriatic Crops and Karst Reclamation, Put Duilova 11, 21000 Split, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8973-1392","authenticated-orcid":false,"given":"Marijana","family":"Popovi\u0107","sequence":"additional","affiliation":[{"name":"Department of Applied Science, Institute for Adriatic Crops and Karst Reclamation, Put Duilova 11, 21000 Split, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tonka","family":"Nin\u010devi\u0107 Runji\u0107","sequence":"additional","affiliation":[{"name":"Department of Plant Sciences, Institute for Adriatic Crops and Karst Reclamation, Put Duilova 11, 21000 Split, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3361-2754","authenticated-orcid":false,"given":"Elda","family":"Vitanovi\u0107","sequence":"additional","affiliation":[{"name":"Department of Applied Science, Institute for Adriatic Crops and Karst Reclamation, Put Duilova 11, 21000 Split, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,7]]},"reference":[{"key":"ref_1","unstructured":"Cavalloro, R. (1983). Fruit Flies of Economic Importance, A.A.Balkema."},{"key":"ref_2","unstructured":"Omkar, I. (2016). Ecofriendly Pest Management for Food Security, Academic Press."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Ezzat, S.M., Jeevanandam, J., Egbuna, C., Merghany, R.M., Akram, M., Daniyal, M., Nisar, J., and Sharif, A. (2019). Semiochemicals: A Green Approach to Pest and Disease Control, Elsevier Inc.","DOI":"10.1016\/B978-0-12-819304-4.00007-5"},{"key":"ref_4","first-page":"1","article-title":"Semiochemicals for Controlling Insect Pests","volume":"59","year":"2019","journal-title":"J. Plant Prot. Res."},{"key":"ref_5","unstructured":"Vonnie, D.C.S. (2017). Biological Control of Pest and Vector Insects, IntechOpen."},{"key":"ref_6","first-page":"459","article-title":"The Use of Semiochemical Slow-Release Devices in Integrated Pest Management Strategies","volume":"15","author":"Heuskin","year":"2011","journal-title":"Biotechnol. Agron. Soc. Environ."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1016\/j.tifs.2015.04.009","article-title":"A Review of Bactrocera Oleae (Rossi) Impact in Olive Products: From the Tree to the Table","volume":"44","author":"Malheiro","year":"2015","journal-title":"Trends Food Sci. Technol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1080\/07352688709382242","article-title":"Plant Volatiles as Insect Attractants","volume":"5","author":"Metcalf","year":"1987","journal-title":"CRC Crit. Rev. Plant Sci."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Shelly, T., Epsky, N., Jang, E.B., Reyes-Flores, J., and Vargas, R. (2014). Trapping and the Detection, Control and Regulation of Tephritid Fruit Flies, Springer.","DOI":"10.1007\/978-94-017-9193-9"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"881","DOI":"10.1007\/BF00985017","article-title":"Olea Europaea Volatiles Attractive and Repellent to the Olive Fruit Fly (Dacus Oleae, Gmelin)","volume":"19","author":"Scarpati","year":"1993","journal-title":"J. Chem. Ecol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1111\/jen.12389","article-title":"Effect of Fruit Volatiles and Light Intensity on the Reproduction of Bactrocera (Dacus) Oleae","volume":"141","author":"Kokkari","year":"2017","journal-title":"J. Appl. Entomol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1111\/jen.12929","article-title":"Determination of Volatile Substances in Olives and Their Effect on Reproduction of the Olive Fruit Fly","volume":"145","author":"Kokkari","year":"2021","journal-title":"J. Appl. Entomol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.phytochem.2015.10.005","article-title":"Identification of Leaf Volatiles from Olive (Olea Europaea) and Their Possible Role in the Ovipositional Preferences of Olive Fly, Bactrocera Oleae (Rossi) (Diptera: Tephritidae)","volume":"121","author":"Malheiro","year":"2016","journal-title":"Phytochemistry"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2020","DOI":"10.1038\/s41598-020-58379-8","article-title":"Olive Fruit Volatiles Route Intraspecific Interactions and Chemotaxis in Bactrocera Oleae (Rossi) (Diptera: Tephritidae) Females","volume":"10","author":"Giunti","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"752","DOI":"10.1093\/jee\/toz341","article-title":"Olive Fruit Fly, Bactrocera Oleae (Diptera: Tephritidae), Attraction to Volatile Compounds Produced by Host and Insect-Associated Yeast Strains","volume":"113","author":"Aldrich","year":"2020","journal-title":"J. Econ. Entomol."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Vitanovi\u0107, E., Lopez, J.M., Aldrich, J.R., \u0160pika, M.J., Boundy-Mills, K., and Zalom, F.G. (2020). Yeasts Associated with the Olive Fruit Fly Bactrocera Oleae (Rossi) (Diptera: Tephritidae) Lead to New Attractants. Agronomy, 10.","DOI":"10.3390\/agronomy10101501"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Bego, A., Burul, F., Popovi\u0107, M., \u0160pika, M.J., Bratin\u010devi\u0107, M.V., Po\u0161\u0107i\u0107, F., and Vitanovi\u0107, E. (2022). Bactrocera Oleae (Rossi) (Diptera: Tephritidae) Response to Different Blends of Olive Fruit Fly-Associated Yeast Volatile Compounds as Attractants. Agronomy, 12.","DOI":"10.3390\/agronomy12010072"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2962","DOI":"10.1002\/ece3.3905","article-title":"Chemical Signaling and Insect Attraction Is a Conserved Trait in Yeasts","volume":"8","author":"Becher","year":"2018","journal-title":"Ecol. Evol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1007\/s00049-020-00317-1","article-title":"Use of Semiochemicals for Surveillance and Control of Hematophagous Insects","volume":"30","author":"Mweresa","year":"2020","journal-title":"Chemoecology"},{"key":"ref_20","unstructured":"(2022, June 01). Available online: https:\/\/pubchem.ncbi.nlm.nih.gov\/."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1007\/s10886-015-0557-y","article-title":"Measure Your Septa Release Ratios: Pheromone Release Ratio Variability Affected by Rubber Septa and Solvent","volume":"41","author":"Kuenen","year":"2015","journal-title":"J. Chem. Ecol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1007\/BF00987710","article-title":"Insect Sex Pheromones: Evaporation Rates of Alcohols and Acetates from Natural Rubber Septa","volume":"7","author":"Butler","year":"1981","journal-title":"J. Chem. Ecol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1080\/01140671.2019.1604554","article-title":"Volatile Compounds as Insect Lures: Factors Affecting Release from Passive Dispenser Systems","volume":"47","author":"Nielsen","year":"2019","journal-title":"New Zeal. J. Crop Hortic. Sci."},{"key":"ref_24","unstructured":"Douglas, M., and Bates, D.G.W. (1988). Nonlinear Regression Analysis and Its Applications, John Wiley & Sons, Inc."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"860","DOI":"10.1007\/s10886-013-0278-z","article-title":"A Survey of Insect Assemblages Responding to Volatiles from a Ubiquitous Fungus in an Agricultural Landscape","volume":"39","author":"Davis","year":"2013","journal-title":"J. Chem. Ecol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"840","DOI":"10.1007\/s10886-013-0306-z","article-title":"Microbial Volatile Emissions as Insect Semiochemicals","volume":"39","author":"Davis","year":"2013","journal-title":"J. Chem. Ecol."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Scolari, F., Valerio, F., Benelli, G., Papadopoulos, N.T., and Van\u00ed\u010dkov\u00e1, L. (2021). Tephritid Fruit Fly Semiochemicals: Current Knowledge and Future Perspectives. Insects, 12.","DOI":"10.3390\/insects12050408"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2072","DOI":"10.1093\/jee\/99.6.2072","article-title":"Olive Fruit Fly (Diptera: Tephritidae) Populations in Relation to Region, Trap Type, Season, and Availability of Fruit","volume":"99","author":"Yokoyama","year":"2006","journal-title":"J. Econ. Entomol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"816","DOI":"10.5958\/0974-4576.2022.00140.2","article-title":"First Study of Ovipositional Preference and Developmental Performance of Bactrocera Oleae (Diptera: Tephritidae) in an Arid Zone (Laghouat: Algeria)","volume":"46","author":"Zineb","year":"2022","journal-title":"J. Entomol. Res."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1603\/008.102.0107","article-title":"Effect of Relative Humidity on Longevity, Ovarian Maturation, and Egg Production in the Olive Fruit Fly (Diptera: Tephritidae)","volume":"102","author":"Broufas","year":"2009","journal-title":"Ann. Entomol. Soc. Am."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1111\/j.1365-2311.1978.tb00908.x","article-title":"Changes in the Ovaries of Olive Flies (Dacus Oleae (Gmelin)) during the Summer, and Their Relationship to Temperature, Humidity and Fruit Availability","volume":"3","author":"Fletcher","year":"1978","journal-title":"Ecol. Entomol."},{"key":"ref_32","unstructured":"Witzgall, P., Mazomenos, B., and Konstantopoulou, M. (2002, January 25\u201329). Alternative Methods for Controlling the Olive Fly, Bactrocera Oleae, Involving Semiochemicals. Proceedings of the IOBC wprs Bulletin Pheromones and Other Semio-Chemicals in Integrated Production, Samos, Greece."},{"key":"ref_33","first-page":"137","article-title":"Attract and Kill of the Olive Fruit Fly Bactrocera Oleae in Greece as a Part of an Integrated Control System","volume":"25","author":"Mazomenos","year":"2002","journal-title":"IOBC WPRS Bull."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1007\/BF02033458","article-title":"A Temperature-Dependent Model for Predicting Release Rates of Pheromone from a Polyethylene Tubing Dispenser","volume":"21","author":"Bradley","year":"1995","journal-title":"J. Chem. Ecol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1111\/eea.12253","article-title":"Gas Chromatography Column as an Ambient-Temperature Volatile Trap","volume":"154","author":"Kuenen","year":"2015","journal-title":"Entomol. Exp. Appl."},{"key":"ref_36","unstructured":"(2022, June 06). DHMZ\u2014Croatian Meteorological and Hydrological Service. Available online: https:\/\/meteo.hr\/index_en.php."},{"key":"ref_37","unstructured":"(2022, July 22). Python Language Reference, Version 3. 8. 12. Python Software Foundation. Available online: http:\/\/www.python.org."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/28\/6\/2431\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:49:51Z","timestamp":1760122191000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/28\/6\/2431"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3,7]]},"references-count":37,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2023,3]]}},"alternative-id":["molecules28062431"],"URL":"https:\/\/doi.org\/10.3390\/molecules28062431","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,3,7]]}}}