{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T19:02:17Z","timestamp":1789498937888,"version":"build-2803163510"},"reference-count":41,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2023,3,7]],"date-time":"2023-03-07T00:00:00Z","timestamp":1678147200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004442","name":"National Science Centre (NCN)","doi-asserted-by":"publisher","award":["2018\/31\/D\/ST4\/01488 (SONATA)"],"award-info":[{"award-number":["2018\/31\/D\/ST4\/01488 (SONATA)"]}],"id":[{"id":"10.13039\/501100004442","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004442","name":"National Science Centre (NCN)","doi-asserted-by":"publisher","award":["POWR.03.02.00-00-I009\/17-00"],"award-info":[{"award-number":["POWR.03.02.00-00-I009\/17-00"]}],"id":[{"id":"10.13039\/501100004442","id-type":"DOI","asserted-by":"publisher"}]},{"name":"European Social Fund","award":["2018\/31\/D\/ST4\/01488 (SONATA)"],"award-info":[{"award-number":["2018\/31\/D\/ST4\/01488 (SONATA)"]}]},{"name":"European Social Fund","award":["POWR.03.02.00-00-I009\/17-00"],"award-info":[{"award-number":["POWR.03.02.00-00-I009\/17-00"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Recently, targeted nanoparticles (NPs) have attracted much attention in cancer treatment due to their high potential as carriers for drug delivery. In this article, we present a novel bioconjugate (DOX\u2013AuNPs\u2013Tmab) consisting of gold nanoparticles (AuNPs, 30 nm) attached to chemotherapeutic agent doxorubicin (DOX) and a monoclonal antibody, trastuzumab (Tmab), which exhibited specific binding to HER2 receptors. The size and shape of synthesized AuNPs, as well as their surface modification, were analyzed by the TEM (transmission electron microscopy) and DLS (dynamic light scattering) methods. Biological studies were performed on the SKOV-3 cell line (HER2+) and showed high specificity of binding to the receptors and internalization capabilities, whereas MDA-MB-231 cells (HER2\u2212) did not. Cytotoxicity experiments revealed a decrease in the metabolic activity of cancer cells and surface area reduction of spheroids treated with DOX\u2013AuNPs\u2013Tmab. The bioconjugate induced mainly cell cycle G2\/M-phase arrest and late apoptosis. Our results suggest that DOX\u2013AuNPs\u2013Tmab has great potential for targeted therapy of HER2-positive tumors.<\/jats:p>","DOI":"10.3390\/molecules28062451","type":"journal-article","created":{"date-parts":[[2023,3,8]],"date-time":"2023-03-08T02:08:14Z","timestamp":1678241294000},"page":"2451","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Doxorubicin- and Trastuzumab-Modified Gold Nanoparticles as Potential Multimodal Agents for Targeted Therapy of HER2+ Cancers"],"prefix":"10.3390","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3104-8704","authenticated-orcid":false,"given":"Kinga","family":"\u017belechowska-Matysiak","sequence":"first","affiliation":[{"name":"Centre of Radiochemistry and Nuclear Chemistry, Institute of Nuclear Chemistry and Technology, 03-195 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3818-5840","authenticated-orcid":false,"given":"Kamil","family":"Wawrowicz","sequence":"additional","affiliation":[{"name":"Centre of Radiochemistry and Nuclear Chemistry, Institute of Nuclear Chemistry and Technology, 03-195 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3623-8929","authenticated-orcid":false,"given":"Mateusz","family":"Wierzbicki","sequence":"additional","affiliation":[{"name":"Department of Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences, 02-787 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tadeusz","family":"Budlewski","sequence":"additional","affiliation":[{"name":"Isotope Therapy Department, Central Clinical Hospital of the Ministry of Interior and Administration, 02-507 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2490-3303","authenticated-orcid":false,"given":"Aleksander","family":"Bilewicz","sequence":"additional","affiliation":[{"name":"Centre of Radiochemistry and Nuclear Chemistry, Institute of Nuclear Chemistry and Technology, 03-195 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9449-6411","authenticated-orcid":false,"given":"Agnieszka","family":"Majkowska-Pilip","sequence":"additional","affiliation":[{"name":"Centre of Radiochemistry and Nuclear Chemistry, Institute of Nuclear Chemistry and Technology, 03-195 Warsaw, Poland"},{"name":"Isotope Therapy Department, Central Clinical Hospital of the Ministry of Interior and Administration, 02-507 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.jconrel.2013.03.018","article-title":"Recent Advances in Theranostic Nanocarriers of Doxorubicin Based on Iron Oxide and Gold Nanoparticles","volume":"169","author":"Gautier","year":"2013","journal-title":"J. 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