{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T10:07:46Z","timestamp":1791022066741,"version":"4.1.0"},"reference-count":37,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2023,3,8]],"date-time":"2023-03-08T00:00:00Z","timestamp":1678233600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001501","name":"UGC grant commission","doi-asserted-by":"publisher","award":["30-434\/2018(BSR)"],"award-info":[{"award-number":["30-434\/2018(BSR)"]}],"id":[{"id":"10.13039\/501100001501","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Shigellosis is a serious foodborne diarrheal disease caused by the Shigella species. It is a critical global health issue. In developing countries, shigellosis causes most of the mortality in children below 5 years of age. Globally, around 165 million cases of diarrhea caused by Shigella are reported, which accounts for almost 1 million deaths, in which the majority are recorded in Third World nations. In this study, silver nanoparticles were synthesized using Mangifera indica kernel (MK-AgNPs) seed extracts. The biosynthesized M. indica silver nanoparticles (MK-AgNPs) were characterized using an array of spectroscopic and microscopic tools, such as UV\u2013Vis, scanning electron microscopy, particle size analyzer, Fourier transform infrared spectroscopy, and X-ray diffractometer. The nanoparticles were spherical in shape and the average size was found to be 42.7 nm. The MK-AgNPs exhibited remarkable antibacterial activity against antibiotic-resistant clinical Shigella sp. The minimum inhibitory concentration (MIC) value of the MK-AgNPs was found to be 20 \u03bcg\/mL against the multi-drug-resistant strain Shigella flexneri. The results clearly demonstrate that MK-AgNPs prepared using M. indica kernel seed extract exhibited significant bactericidal action against pathogenic Shigella species. The biosynthesized nanoparticles from mango kernel could possibly prove therapeutically useful and effective in combating the threat of shigellosis after careful investigation of its toxicity and in vivo efficacy.<\/jats:p>","DOI":"10.3390\/molecules28062468","type":"journal-article","created":{"date-parts":[[2023,3,9]],"date-time":"2023-03-09T01:35:37Z","timestamp":1678325737000},"page":"2468","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Biogenic Preparation and Characterization of Silver Nanoparticles from Seed Kernel of Mangifera indica and Their Antibacterial Potential against Shigella spp."],"prefix":"10.3390","volume":"28","author":[{"given":"Sudha","family":"Angamuthu","sequence":"first","affiliation":[{"name":"Bon Secours Arts & Science College for Women, Department of Biotechnology, Sowthapuram (PO), Near Veppadai, Namakkal 638008, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3500-8681","authenticated-orcid":false,"given":"Selvankumar","family":"Thangaswamy","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Mahendra Arts and Science College (Autonomous), Namakkal 637501, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amutha","family":"Raju","sequence":"additional","affiliation":[{"name":"Centre for Post Graduate and Research Studies, Periyar University, Salem 636001, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6598-4328","authenticated-orcid":false,"given":"Fohad Mabood","family":"Husain","sequence":"additional","affiliation":[{"name":"Department of Food Science and Nutrition, King Saud University, Riyadh 11495, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8047-9231","authenticated-orcid":false,"given":"Bilal","family":"Ahmed","sequence":"additional","affiliation":[{"name":"School of Chemical Engineering, Yeungnam University, Gyeongsan 38541, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nasser A.","family":"Al-Shabib","sequence":"additional","affiliation":[{"name":"Department of Food Science and Nutrition, King Saud University, Riyadh 11495, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed Jamal","family":"Hakeem","sequence":"additional","affiliation":[{"name":"Department of Food Science and Nutrition, King Saud University, Riyadh 11495, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Syed Ali","family":"Shahzad","sequence":"additional","affiliation":[{"name":"Department of Food Science and Nutrition, King Saud University, Riyadh 11495, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9291-1634","authenticated-orcid":false,"given":"Saud A.","family":"Abudujayn","sequence":"additional","affiliation":[{"name":"Department of Food Science and Nutrition, King Saud University, Riyadh 11495, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2864-1649","authenticated-orcid":false,"given":"Suliman Y.","family":"Alomar","sequence":"additional","affiliation":[{"name":"Department of Zoology, King Saud University, Riyadh 11495, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1016\/j.jiph.2017.09.025","article-title":"Emergence of antibiotic resistant Shigella species: A matter of concern","volume":"11","author":"Puzari","year":"2018","journal-title":"J. Infect. Public Health"},{"key":"ref_2","first-page":"793","article-title":"A review on shigellosis: Pathogenesis and antibiotic resistance","volume":"14","author":"Salman","year":"2020","journal-title":"Drug Invent. Today"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Bai, X., Li, X., Liu, X., Xing, Z., Su, R., Wang, Y., Xia, X., and Shi, C. (2022). Antibacterial Effect of Eugenol on Shigella flexneri and Its Mechanism. Foods, 11.","DOI":"10.3390\/foods11172565"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"565","DOI":"10.4103\/0971-5916.187104","article-title":"Shigellosis: Epidemiology in India","volume":"143","author":"Taneja","year":"2016","journal-title":"Indian J. Med. Res."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"162","DOI":"10.29252\/JABR.05.04.05","article-title":"In Vitro Evaluation of Antibacterial Properties of Zinc Oxide Nanoparticles on Pathogenic Prokaryotes","volume":"5","author":"Nazoori","year":"2018","journal-title":"J. Appl. Biotechnol. Rep."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"3137","DOI":"10.2147\/IDR.S219755","article-title":"Shigella: Antibiotic-Resistance Mechanisms and New Horizons for Treatment","volume":"12","author":"Ranjbar","year":"2019","journal-title":"Infect. Drug Resist."},{"key":"ref_7","first-page":"9671594","article-title":"Green Synthesis of Silver Nanoparticles Using the Tridax procumbens Plant Extract and Screening of Its Antimicrobial and Anticancer Activities","volume":"2022","author":"Pungle","year":"2022","journal-title":"Hindawi Oxidative Med. Cell. Longev."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.procbio.2021.05.024","article-title":"Metabolic annotation, interactions and characterization of natural products of mango (Mangifera indica L.): 1H NMR based chemical metabolomics profiling","volume":"108","author":"Angamuthu","year":"2021","journal-title":"Process Biochem."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Sharma, G., Nam, J.-S., Sharma, A., and Lee, S.-S. (2018). Antimicrobial Potential of Silver Nanoparticles Synthesized Using Medicinal Herb Coptidis rhizome. Molecules, 23.","DOI":"10.3390\/molecules23092268"},{"key":"ref_10","first-page":"4","article-title":"Mango seed kernel, a highly nutritious food, should we continue to trash or use?","volume":"7","author":"Patel","year":"2018","journal-title":"J. Pharmacogn. Phytochem."},{"key":"ref_11","first-page":"3728","article-title":"Effect of processing on proximate and phytochemical content of mango (Mangifera indica) kernel","volume":"6","author":"Gumte","year":"2018","journal-title":"Int. J. Chem. Stud."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1016\/S1995-7645(11)60089-8","article-title":"Anti-enteric bacterial activity and phytochemical analysis of the seed kernel extract of Mangifera indica Linnaeus against Shigella dysenteriae (Shiga, corrig.) Castellani and Chalmers","volume":"4","author":"Rajan","year":"2011","journal-title":"Asian Pac. J. Trop. Med."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1016\/j.foodres.2011.11.008","article-title":"Fast LC\u2013MS analysis of gallotannins from mango (Mangifera indica L.) kernels and effects of methanolysis on their antibacterial activity and iron binding capacity","volume":"45","author":"Engels","year":"2012","journal-title":"Food Res. Int."},{"key":"ref_14","first-page":"75","article-title":"Anti-Salmonella activities of Mangifera indica seed kernel aqueous extract (MISKAE)","volume":"6","author":"Azhagesan","year":"2015","journal-title":"Adv. Appl. Sci. Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2536","DOI":"10.1007\/s13197-019-03732-7","article-title":"Extraction of bioactive compounds from mango (Mangifera indica L. var. Carabao) seed kernel with ethanol\u2013water binary solvent systems","volume":"56","author":"Lim","year":"2019","journal-title":"J. Food Sci. Technol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.indcrop.2013.04.001","article-title":"Mango peel extract mediated novel route for synthesis of silver nanoparticles and antibacterial application of silver nanoparticles loaded onto non-woven fabrics","volume":"48","author":"Yang","year":"2013","journal-title":"Ind. Crop. Prod."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1007\/s40204-017-0067-9","article-title":"Green synthesis and characterization of Ag nanoparticles from Mangifera indica leaves for dental restoration and antibacterial applications","volume":"6","author":"Sundeep","year":"2017","journal-title":"Prog. Biomater."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"102970","DOI":"10.1016\/j.bioorg.2019.102970","article-title":"Phytosynthesis of silver nanoparticles using Mangifera indica flower extract as bioreductant and their broad-spectrum antibacterial activity","volume":"88","author":"Ameen","year":"2019","journal-title":"Bioorg. Chem."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"157","DOI":"10.29252\/JABR.05.04.04","article-title":"The Biological Synthesis of Silver Nanoparticles by Mango Plant Extract and Its Anti-Candida Effects","volume":"5","author":"Salati","year":"2018","journal-title":"J. Appl. Biotechnol. Rep."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Navarro, M., Arnaez, E., Moreira, I., Quesada, S., Azofeifa, G., Wilhelm, K., Vargas, F., and Chen, P. (2019). Polyphenolic Characterization, Antioxidant, and Cytotoxic Activities of Mangifera indica Cultivars from Costa Rica. Foods, 8.","DOI":"10.3390\/foods8090384"},{"key":"ref_21","first-page":"292","article-title":"Facile green synthesis of silver nanoparticles using Mangifera indica seed aqueous extract and its antimicrobial, antioxidant and cytotoxic potential (3-in-1 system). ARTIF","volume":"49","author":"Donga","year":"2021","journal-title":"Cells Nanomed. Biotechnol."},{"key":"ref_22","first-page":"1","article-title":"Identification and quantification of phenolic compounds in Mangifera indica waterlily kernel and their free radical scavenging activity","volume":"2","author":"Abdullah","year":"2015","journal-title":"J. Adv. Agric. Technol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1315","DOI":"10.1080\/21691401.2020.1843470","article-title":"Antimicrobial, antioxidant and anticancer activities of gold nanoparticles green synthesized using Mangifera indica seed aqueous extract","volume":"48","author":"Donga","year":"2020","journal-title":"Artif. Cells Nanomed. Biotechnol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1080\/21691401.2017.1414826","article-title":"Augmented photocatalytic, antibacterial and antifungal activity of prunosynthetic silver nanoparticles","volume":"46","author":"Jaffri","year":"2018","journal-title":"Artif. Cells Nanomed. Biotechnol."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Xing, Y., Liao, X., Liu, X., Li, W., Huang, R., Tang, J., Xu, Q., Li, X., and Yu, J. (2021). Characterization and Antimicrobial Activity of Silver Nanoparticles Synthesized with the Peel Extract of Mango. Materials, 14.","DOI":"10.3390\/ma14195878"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1347","DOI":"10.1093\/jac\/dks061","article-title":"Cephalosporin-resistant Shigella flexneri over 9 years (2001\u20132009) in India","volume":"67","author":"Taneja","year":"2012","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1016\/j.msec.2015.01.035","article-title":"Sreedhara Reddy, Green synthesis and characterization of silver nanoparticles using Lantana camara leaf extract","volume":"49","author":"Ajitha","year":"2015","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1007\/s12010-022-04120-2","article-title":"Potential Antimicrobial and Antibiofilm Properties of Copper Oxide Nanoparticles: Time-Kill Kinetic Essay and Ultrastructure of Pathogenic Bacterial Cells","volume":"195","author":"Shehabeldine","year":"2022","journal-title":"Appl. Biochem. Biotechnol."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Salem, S.S., Hashem, A.H., Sallam AA, M., Doghish, A.S., Al-Askar, A.A., Arishi, A.A., and Shehabeldine, A.M. (2022). Synthesis of silver nanocomposite based on carboxymethyl cellulose: Antibacterial, antifungal and anticancer activities. Polymers, 14.","DOI":"10.3390\/polym14163352"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1303","DOI":"10.4314\/njt.v36i4.41","article-title":"Potential use of Mangifera indica seed kernel and Citrus aurantiifolia seed in water disinfection","volume":"36","author":"Seghosime","year":"2018","journal-title":"Niger. J. Technol."},{"key":"ref_31","first-page":"214","article-title":"Minimum bactericidal concentration of chemically synthesized silver nanoparticles against pathogenic Salmonella and Shigella strains isolated from layer poultry farms","volume":"7","author":"Omara","year":"2017","journal-title":"J. Appl. Pharm. Sci."},{"key":"ref_32","first-page":"88","article-title":"Synthesis and characterization of Silver nanoparticles from Cinnamomum tamala leaf extract and its antibacterial potential","volume":"11","author":"Nahar","year":"2020","journal-title":"Int. J. Nano Dimens."},{"key":"ref_33","first-page":"214","article-title":"Evaluation of Mango Seed kernel Extract as natural occurring phenolic rich antioxidant compound","volume":"48","author":"Toliba","year":"2016","journal-title":"Bull. Natl. Nutr. Inst. Arab. Repub. Egypt"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1007\/s10876-020-01827-2","article-title":"Protein Leakage Induced Marine Antibiofouling Activity of Biosynthesized Zinc Oxide Nanoparticles","volume":"32","author":"Dharmaraj","year":"2021","journal-title":"J. Clust. Sci."},{"key":"ref_35","first-page":"1906","article-title":"Murraya koenigii mediated silver nanoparticle synthesis and its activity against enteric pathogens","volume":"10","author":"Amutha","year":"2019","journal-title":"Int. J. Pharm. Sci. Res."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"93","DOI":"10.4314\/ejhs.v26i2.2","article-title":"Antibiotic Resistance Patterns of Common Gram-negative Uropathogens in St. Paul\u2019s Hospital Millennium Medical College","volume":"26","author":"Mamuye","year":"2016","journal-title":"Ethiop. J. Health Sci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1488","DOI":"10.1080\/21691401.2017.1374283","article-title":"Rapid biosynthesis of Bauhinia variegata flower extract-mediated silver nanoparticles: An effective antioxidant scavenger and \u03b1-amylase inhibitor","volume":"46","author":"Johnson","year":"2018","journal-title":"Artif. Cells Nanomed. Biotechnol."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/28\/6\/2468\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:50:49Z","timestamp":1760122249000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/28\/6\/2468"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3,8]]},"references-count":37,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2023,3]]}},"alternative-id":["molecules28062468"],"URL":"https:\/\/doi.org\/10.3390\/molecules28062468","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,3,8]]}}}