{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T01:38:56Z","timestamp":1792805936043,"version":"4.5.0"},"reference-count":65,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2023,3,9]],"date-time":"2023-03-09T00:00:00Z","timestamp":1678320000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key R&amp;D Program of China","doi-asserted-by":"publisher","award":["2021YFC2100201"],"award-info":[{"award-number":["2021YFC2100201"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key R&amp;D Program of China","doi-asserted-by":"publisher","award":["SHDC2020CR3076B"],"award-info":[{"award-number":["SHDC2020CR3076B"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Municipal Hospital Clinical Skills and Innovation Fund (2020\u20132022) of the Shanghai Hospital Development Center","award":["2021YFC2100201"],"award-info":[{"award-number":["2021YFC2100201"]}],"id":[{"id":"https:\/\/ror.org\/02zrtve16","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Municipal Hospital Clinical Skills and Innovation Fund (2020\u20132022) of the Shanghai Hospital Development Center","award":["SHDC2020CR3076B"],"award-info":[{"award-number":["SHDC2020CR3076B"]}],"id":[{"id":"https:\/\/ror.org\/02zrtve16","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Most osteoporosis (OP) fracture accidents in men are due not only to a low BMD but also because of unhealthy muscle support. However, there has been a limited number of reports about how muscle metabolism is disturbed by OP in males. In this work, a pathway analysis based on metabolomic research was carried out to fill this gap. A classical orchiectomy procedure was adapted to create an OP animal model. A micro-CT and pathological section were applied for a bone and muscle phenotype assessment and a pathology analysis. UPLC-Q-TOF\/MS and UPLC-QQQ-MS\/MS were applied to measure metabolites in skeletal muscle samples among groups. In total, 31 significantly differential metabolites were detected by comparing healthy models and OP animals, and 7 representative metabolites among the 31 significantly differential metabolites were identified and validated experimentally by UPLC-QQQ-MS\/MS (xanthine, L-phenylalanine, choline, hypoxanthine, L-tryptophan, succinic acid, and L-tyrosine). An ingenuity pathway analysis (IPA) analysis revealed significantly enriched pathways involved in inflammation, oxidative stress, and necrosis. To our best knowledge, this is the first study to investigate early muscle disorder processes in Cases of OP at a metabolic level, facilitating early intervention and protection from OP fractures for aged men.<\/jats:p>","DOI":"10.3390\/molecules28062512","type":"journal-article","created":{"date-parts":[[2023,3,10]],"date-time":"2023-03-10T02:05:54Z","timestamp":1678413954000},"page":"2512","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Integrating Untargeted and Targeted Metabolomics Coupled with Pathway Analysis Reveals Muscle Disorder in Osteoporosis on Orchiectomized Mice"],"prefix":"10.3390","volume":"28","author":[{"given":"Fei","family":"Ge","sequence":"first","affiliation":[{"name":"College of Sciences, Shanghai University, Shanghai 200444, China"},{"name":"School of Medicine, Shanghai University, Shanghai 200444, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziheng","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Orthopedics, Shanghai General Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 201620, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanting","family":"Che","sequence":"additional","affiliation":[{"name":"College of Sciences, Shanghai University, Shanghai 200444, China"},{"name":"School of Medicine, Shanghai University, Shanghai 200444, China"},{"name":"Institute of Translational Medicine, Shanghai University, Shanghai 200444, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingqing","family":"Qian","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, The Fifth People\u2019s Hospital of Shanghai, Fudan University, Shanghai 201100, China"},{"name":"National Institute of Clinical Research, The Fifth People\u2019s Hospital of Shanghai, Fudan University, Shanghai 201100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinfei","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, The Fifth People\u2019s Hospital of Shanghai, Fudan University, Shanghai 201100, China"},{"name":"National Institute of Clinical Research, The Fifth People\u2019s Hospital of Shanghai, Fudan University, Shanghai 201100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2675-3265","authenticated-orcid":false,"given":"Hongxia","family":"Zhao","sequence":"additional","affiliation":[{"name":"School of Medicine, Shanghai University, Shanghai 200444, China"},{"name":"Zhanjiang Institute of Clinical Medicine, Central People\u2019s Hospital of Zhanjiang, Zhanjiang 524037, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8050-7167","authenticated-orcid":false,"given":"Si","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Dong","sequence":"additional","affiliation":[{"name":"School of Medicine, Shanghai University, Shanghai 200444, China"},{"name":"Institute of Translational Medicine, Shanghai University, Shanghai 200444, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.bone.2016.09.011","article-title":"Androgens have antiresorptive effects on trabecular disuse osteopenia independent from muscle atrophy","volume":"93","author":"Laurent","year":"2016","journal-title":"Bone"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1177\/0897190010397716","article-title":"Osteoporosis in men: A review of endogenous sex hormones and testosterone replacement therapy","volume":"24","author":"Martin","year":"2011","journal-title":"J. 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