{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T16:31:12Z","timestamp":1789576272259,"version":"build-2803163510"},"reference-count":69,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2023,3,13]],"date-time":"2023-03-13T00:00:00Z","timestamp":1678665600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32172154"],"award-info":[{"award-number":["32172154"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["LYG06521202107"],"award-info":[{"award-number":["LYG06521202107"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["2020TD68"],"award-info":[{"award-number":["2020TD68"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"521 Program","award":["32172154"],"award-info":[{"award-number":["32172154"]}]},{"name":"521 Program","award":["LYG06521202107"],"award-info":[{"award-number":["LYG06521202107"]}]},{"name":"521 Program","award":["2020TD68"],"award-info":[{"award-number":["2020TD68"]}]},{"name":"Basic Scientific Research of China Academy of Fishery Sciences","award":["32172154"],"award-info":[{"award-number":["32172154"]}]},{"name":"Basic Scientific Research of China Academy of Fishery Sciences","award":["LYG06521202107"],"award-info":[{"award-number":["LYG06521202107"]}]},{"name":"Basic Scientific Research of China Academy of Fishery Sciences","award":["2020TD68"],"award-info":[{"award-number":["2020TD68"]}]},{"name":"Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)","award":["32172154"],"award-info":[{"award-number":["32172154"]}]},{"name":"Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)","award":["LYG06521202107"],"award-info":[{"award-number":["LYG06521202107"]}]},{"name":"Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)","award":["2020TD68"],"award-info":[{"award-number":["2020TD68"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Misgurnus anguillicaudatus (loach) is a widely distributed benthic fish in Asia. In this study, the alkaline protease was used to hydrolyze loach, and the hydrolysate products of different molecular weights were obtained by membrane separation. In vitro antioxidant assays showed that the &lt;3 kDa fraction (SLH-1) exhibited the strongest antioxidant activity (DPPH, hydroxyl radical and superoxide radical scavenging ability, and reducing power), while SLH-1 was purified by gel filtration chromatography, and peptide sequences were identified by LC-MS\/MS. A total of six peptides with antioxidant activity were identified, namely SERDPSNIKWGDAGAQ (D-1), TVDGPSGKLWR (D-2), NDHFVKL (D-3), AFRVPTP (D-4), DAGAGIAL (D-5), and VSVVDLTVR (D-6). In vitro angiotensin-converting enzyme (ACE) inhibition assay and pancreatic cholesterol esterase (CE) inhibition assay, peptide D-4 (IC50 95.07 \u03bcg\/mL, 0.12 mM) and D-2 inhibited ACE, and peptide D-2 (IC50 3.19 mg\/mL, 2.62 mM), D-3, and D-6 acted as pancreatic CE inhibitors. The inhibitory mechanisms of these peptides were investigated by molecular docking. The results showed that the peptides acted by binding to the key amino acids of the catalytic domain of enzymes. These results could provide the basis for the nutritional value and promote the type of healthy products from hydrolyzed loach.<\/jats:p>","DOI":"10.3390\/molecules28062589","type":"journal-article","created":{"date-parts":[[2023,3,13]],"date-time":"2023-03-13T03:28:33Z","timestamp":1678678113000},"page":"2589","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Multiple Bioactivities of Peptides from Hydrolyzed Misgurnus anguillicaudatus"],"prefix":"10.3390","volume":"28","author":[{"given":"Baojie","family":"Dou","sequence":"first","affiliation":[{"name":"Jiangsu Key Laboratory of Marine Bioresources and Environment\/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, China"},{"name":"Co-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang 222005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6939-7008","authenticated-orcid":false,"given":"Xudong","family":"Wu","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Marine Bioresources and Environment\/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, China"},{"name":"Co-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang 222005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zihan","family":"Xia","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Marine Bioresources and Environment\/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, China"},{"name":"Co-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang 222005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guanghao","family":"Wu","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Marine Bioresources and Environment\/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, China"},{"name":"Co-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang 222005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quanyou","family":"Guo","sequence":"additional","affiliation":[{"name":"East China Sea Fishery Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4409-3766","authenticated-orcid":false,"given":"Mingsheng","family":"Lyu","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Marine Bioresources and Environment\/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, China"},{"name":"Co-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang 222005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shujun","family":"Wang","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Marine Bioresources and Environment\/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, China"},{"name":"Co-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang 222005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"110161","DOI":"10.1016\/j.foodres.2021.110161","article-title":"Production, characterization and molecular docking of antioxidant peptides from peptidome of kinema fermented with proteolytic Bacillus spp","volume":"141","author":"Sanjukta","year":"2021","journal-title":"Food Res. Int."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"100165","DOI":"10.1016\/j.fochx.2021.100165","article-title":"Identification and characterization of cholesterol esterase and lipase inhibitory peptides from amaranth protein hydrolysates","volume":"12","author":"Mudgil","year":"2021","journal-title":"Food Chem. X"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"112221","DOI":"10.1016\/j.lwt.2021.112221","article-title":"Identification of angiotensin converting enzyme (ACE) inhibitory and antioxidant peptides derived from Pixian broad bean paste","volume":"151","author":"Li","year":"2021","journal-title":"LWT-Food Sci. Technol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"4994","DOI":"10.1039\/D0FO00880J","article-title":"Isolation and identification of cholesterol esterase and pancreatic lipase inhibitory peptides from brewer\u2019s spent grain by consecutive chromatography and mass spectrometry","volume":"11","author":"Garzon","year":"2020","journal-title":"Food Funct."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"101887","DOI":"10.1016\/j.redox.2021.101887","article-title":"Redox changes in obesity, metabolic syndrome, and diabetes","volume":"42","author":"Korac","year":"2021","journal-title":"Redox Biol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1016\/j.tifs.2022.11.024","article-title":"Purification and fractionation of bioactive peptides through membrane filtration: A critical and application review","volume":"131","author":"Alavi","year":"2023","journal-title":"Trends Food Sci. Technol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"134136","DOI":"10.1016\/j.foodchem.2022.134136","article-title":"Generation of novel antioxidant peptides from silver carp muscle hydrolysate: Gastrointestinal digestion stability and transepithelial absorption property","volume":"403","author":"Wang","year":"2023","journal-title":"Food Chem."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"100231","DOI":"10.1016\/j.fochx.2022.100231","article-title":"Characterization of ACE inhibitory and antioxidant peptides in yak and cow milk hard chhurpi cheese of the Sikkim Himalayan region","volume":"13","author":"Abedin","year":"2022","journal-title":"Food Chem. X"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"134481","DOI":"10.1016\/j.foodchem.2022.134481","article-title":"Novel bioactive peptides from ginkgo biloba seed protein and evaluation of their alpha-glucosidase inhibition activity","volume":"404","author":"Wang","year":"2023","journal-title":"Food Chem."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"7393","DOI":"10.3168\/jds.2020-19868","article-title":"New insights into the cholesterol esterase- and lipase-inhibiting potential of bioactive peptides from camel whey hydrolysates: Identification, characterization, and molecular interaction","volume":"104","author":"Baba","year":"2021","journal-title":"J. Dairy Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"101873","DOI":"10.1016\/j.fbio.2022.101873","article-title":"Immunomodulatory effects of egg white peptides on immunosuppressed mice and sequence identification of immunomodulatory peptides","volume":"49","author":"Chen","year":"2022","journal-title":"Food Biosci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"104677","DOI":"10.1016\/j.jff.2021.104677","article-title":"High-degree hydrolysis sea cucumber peptides improve exercise performance and exert antifatigue effect via activating the NRF2 and AMPK signaling pathways in mice","volume":"86","author":"Wang","year":"2021","journal-title":"J. Funct. Foods"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"114384","DOI":"10.1016\/j.lwt.2022.114384","article-title":"Extraction, identification and anti-photoaging activity evaluation of collagen peptides from silver carp (Hypophthalmichthys molitrix) skin","volume":"173","author":"Huang","year":"2023","journal-title":"LWT-Food Sci. Technol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"113636","DOI":"10.1016\/j.lwt.2022.113636","article-title":"Novel antioxidant peptides from protein hydrolysates of scallop (Argopecten irradians) mantle using enzymatic and microbial methods: Preparation, purification, identification and characterization","volume":"164","author":"Zhi","year":"2022","journal-title":"LWT-Food Sci. Technol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"113196","DOI":"10.1016\/j.lwt.2022.113196","article-title":"Novel ACE inhibitory, antioxidant and \u03b1-glucosidase inhibitory peptides identified from fermented rubing cheese through peptidomic and molecular docking","volume":"159","author":"Wei","year":"2022","journal-title":"LWT-Food Sci. Technol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"131395","DOI":"10.1016\/j.foodchem.2021.131395","article-title":"Exploration of bioactive peptides from various origin as promising nutraceutical treasures: In vitro, in silico and in vivo studies","volume":"373","author":"Manzoor","year":"2022","journal-title":"Food Chem."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"109126","DOI":"10.1016\/j.lwt.2020.109126","article-title":"Optimization of pea protein hydrolysate preparation and purification of antioxidant peptides based on an in silico analytical approach","volume":"123","author":"Ding","year":"2020","journal-title":"LWT-Food Sci. Technol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"100062","DOI":"10.1016\/j.fochms.2021.100062","article-title":"Isolation, purification and characterization of the antibacterial, antihypertensive and antioxidative properties of the bioactive peptides in the purified and proteolyzed major storage protein of pigeon pea (Cajanus cajan) seeds","volume":"4","author":"Bravo","year":"2022","journal-title":"Food Chem. Mol. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"10748","DOI":"10.3168\/jds.2019-16520","article-title":"Identification and molecular docking study of novel cholesterol esterase inhibitory peptides from camel milk proteins","volume":"102","author":"Mudgil","year":"2019","journal-title":"J. Dairy Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1016\/j.tifs.2021.02.031","article-title":"Production, bioactive properties, and potential applications of fish protein hydrolysates: Developments and challenges","volume":"110","author":"Gao","year":"2021","journal-title":"Trends Food Sci. Technol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.fbio.2018.11.007","article-title":"Antioxidant activity of Sind sardine hydrolysates with pistachio green hull (PGH) extracts","volume":"27","author":"Sarteshnizi","year":"2019","journal-title":"Food Biosci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1016\/j.jff.2013.12.024","article-title":"Antioxidant and cryoprotective effects of a tetrapeptide isolated from Amur sturgeon skin gelatin","volume":"7","author":"Nikoo","year":"2014","journal-title":"J. Funct. Foods"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"126809","DOI":"10.1016\/j.foodchem.2020.126809","article-title":"Identification and characterization of novel antioxidant peptides from mackerel (Scomber japonicus) muscle protein hydrolysates","volume":"323","author":"Bashir","year":"2020","journal-title":"Food Chem."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Pantiora, P., Furlan, V., Matiadis, D., Mavroidi, B., Perperopoulou, F., Papageorgiou, A.C., Sagnou, M., Bren, U., Pelecanou, M., and Labrou, N.E. (2022). Monocarbonyl Curcumin Analogues as Potent Inhibitors against Human Glutathione Transferase P1-1. Antioxidants, 12.","DOI":"10.3390\/antiox12010063"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Furlan, V., and Bren, U. (2021). Insight into Inhibitory Mechanism of PDE4D by Dietary Polyphenols Using Molecular Dynamics Simulations and Free Energy Calculations. Biomolecules, 11.","DOI":"10.3390\/biom11030479"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1016\/j.ifset.2008.08.007","article-title":"Effect of degree of hydrolysis on the antioxidant activity of loach (Misgurnus anguillicaudatus) protein hydrolysates","volume":"10","author":"You","year":"2009","journal-title":"Innov. Food Sci. Emerg. Technol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"7948","DOI":"10.1021\/jf2016368","article-title":"Antioxidant and antiproliferative activities of loach (Misgurnus anguillicaudatus) peptides prepared by papain digestion","volume":"59","author":"You","year":"2011","journal-title":"J. Agric. Food Chem."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"885","DOI":"10.1111\/jfb.12307","article-title":"cDNA cloning and expression characterization of serum transferrin gene from oriental weatherfish Misgurnus anguillicaudatus","volume":"84","author":"Chen","year":"2014","journal-title":"J. Fish Biol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1016\/j.aquaculture.2018.07.061","article-title":"Effect of farming patterns on the nutrient composition and farming environment of loach, Paramisgurnus dabryanus","volume":"497","author":"Dong","year":"2018","journal-title":"Aquaculture"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"111844","DOI":"10.1016\/j.lwt.2021.111844","article-title":"Characterization and film-forming properties of acid soluble collagens from different by-products of loach (Misgurnus anguillicaudatus)","volume":"149","author":"Sun","year":"2021","journal-title":"LWT-Food Sci. Technol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1167","DOI":"10.1016\/j.foodres.2010.02.009","article-title":"Purification and identification of antioxidative peptides from loach (Misgurnus anguillicaudatus) protein hydrolysate by consecutive chromatography and electrospray ionization-mass spectrometry","volume":"43","author":"You","year":"2010","journal-title":"Food Res. Int."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1016\/j.foodchem.2010.06.007","article-title":"In vitro antioxidant activity and in vivo anti-fatigue effect of loach (Misgurnus anguillicaudatus) peptides prepared by papain digestion","volume":"124","author":"You","year":"2011","journal-title":"Food Chem."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"129399","DOI":"10.1016\/j.foodchem.2021.129399","article-title":"Preparation and identification of antioxidant peptides from cottonseed proteins","volume":"352","author":"Wang","year":"2021","journal-title":"Food Chem."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"109215","DOI":"10.1016\/j.lwt.2020.109215","article-title":"Purification and characterization of novel antioxidant peptides from duck breast protein hydrolysates","volume":"125","author":"Li","year":"2020","journal-title":"LWT-Food Sci. Technol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1002\/jlb.65.3.337","article-title":"Signal transduction by reactive oxygen species in non-phagocytic cells","volume":"65","author":"Finkel","year":"1999","journal-title":"J. Leukoc. Biol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1297","DOI":"10.2174\/1381612033454892","article-title":"Food-derived Bioactive Peptides\u2014Opportunities for Designing Future Foods","volume":"9","author":"Korhonen","year":"2003","journal-title":"Curr. Pharm. Des."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"102100","DOI":"10.1016\/j.bcab.2021.102100","article-title":"In-vitro angiotensin converting enzyme (ACE), antioxidant activity and some functional properties of silver catfish (Pangasius sp.) protein hydrolysate by ultrafiltration","volume":"35","author":"Shaik","year":"2021","journal-title":"Biocatal. Agric. Biotechnol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"128339","DOI":"10.1016\/j.foodchem.2020.128339","article-title":"Antioxidant mechanism, antibacterial activity, and functional characterization of peptide fractions obtained from barred mackerel gelatin with a focus on application in carbonated beverages","volume":"342","author":"Kim","year":"2021","journal-title":"Food Chem."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"129068","DOI":"10.1016\/j.foodchem.2021.129068","article-title":"Exploring the potential of novel xanthine oxidase inhibitory peptide (ACECD) derived from Skipjack tuna hydrolysates using affinity-ultrafiltration coupled with HPLC-MALDI-TOF\/TOF-MS","volume":"347","author":"Zhong","year":"2021","journal-title":"Food Chem."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.jff.2017.05.029","article-title":"Food matrix interaction and bioavailability of bioactive peptides: Two faces of the same coin?","volume":"35","author":"Udenigwe","year":"2017","journal-title":"J. Funct. Foods"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"7533","DOI":"10.1039\/C9FO01858A","article-title":"A new dual-peptide strategy for enhancing antioxidant activity and exploring the enhancement mechanism","volume":"10","author":"Ma","year":"2019","journal-title":"Food Funct."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"33","DOI":"10.4014\/jmb.2006.06002","article-title":"In Vivo\/In Vitro Properties of Novel Antioxidant Peptide from Pinctada fucata","volume":"31","author":"Ma","year":"2021","journal-title":"J. Microbiol. Biotechnol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"111359","DOI":"10.1016\/j.foodres.2022.111359","article-title":"Purification, identification, and antioxidative mechanism of three novel selenium-enriched oyster antioxidant peptides","volume":"157","author":"Xia","year":"2022","journal-title":"Food Res. Int."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"130999","DOI":"10.1016\/j.foodchem.2021.130999","article-title":"Structure-guided discovery of antioxidant peptides bounded to the Keap1 receptor as hunter for potential dietary antioxidants","volume":"373","author":"Yin","year":"2022","journal-title":"Food Chem."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"696","DOI":"10.1016\/j.jfda.2017.07.008","article-title":"Production of antioxidant and ACE-inhibitory peptides from Kluyveromyces marxianus protein hydrolysates: Purification and molecular docking","volume":"26","author":"Mirzaei","year":"2018","journal-title":"J. Food Drug Anal."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.lwt.2016.08.047","article-title":"A novel antioxidant and ACE inhibitory peptide from rice bran protein: Biochemical characterization and molecular docking study","volume":"75","author":"Wang","year":"2017","journal-title":"LWT-Food Sci. Technol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"113287","DOI":"10.1016\/j.lwt.2022.113287","article-title":"Isolation and characterization, antioxidant, and antihypertensive activity of novel bioactive peptides derived from hydrolysis of King Boletus mushroom","volume":"160","author":"Kaprasob","year":"2022","journal-title":"LWT-Food Sci. Technol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"11234","DOI":"10.1021\/jf202902r","article-title":"Inhibition strength of short peptides derived from an ACE inhibitory peptide","volume":"59","author":"Terashima","year":"2011","journal-title":"J. Agric. Food Chem."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1016\/j.foodchem.2016.10.087","article-title":"Identification, functional gastrointestinal stability and molecular docking studies of lentil peptides with dual antioxidant and angiotensin I converting enzyme inhibitory activities","volume":"221","author":"Penas","year":"2017","journal-title":"Food Chem."},{"key":"ref_50","first-page":"2113","article-title":"Lipid-Lowering mechanisms of grape seed extract (Vitis vinifera L.) and its antihyperlidemic activity","volume":"40","author":"Adisakwattana","year":"2010","journal-title":"J. Med. Plants Res."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"130661","DOI":"10.1016\/j.foodchem.2021.130661","article-title":"A comparative investigation into novel cholesterol esterase and pancreatic lipase inhibitory peptides from cow and camel casein hydrolysates generated upon enzymatic hydrolysis and in-vitro digestion","volume":"367","author":"Mudgil","year":"2022","journal-title":"Food Chem."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1016\/j.procbio.2019.12.022","article-title":"Involvement of Nrf2 and Keap1 in the activation of antioxidant responsive element (ARE) by chemopreventive agent peptides from soft-shelled turtle","volume":"92","author":"Wang","year":"2020","journal-title":"Process Biochem."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"112532","DOI":"10.1016\/j.ejmech.2020.112532","article-title":"Recent progress in Keap1-Nrf2 protein-protein interaction inhibitors","volume":"202","author":"Mou","year":"2020","journal-title":"Eur. J. Med. Chem."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"3043","DOI":"10.1039\/C9FO03018B","article-title":"Studies on the bioactivities and molecular mechanism of antioxidant peptides by 3D-QSAR, in vitro evaluation and molecular dynamic simulations","volume":"11","author":"Yan","year":"2020","journal-title":"Food Funct."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"127921","DOI":"10.1016\/j.foodchem.2020.127921","article-title":"Identification of novel antioxidant peptides from snakehead (Channa argus) soup generated during gastrointestinal digestion and insights into the anti-oxidation mechanisms","volume":"337","author":"Zhang","year":"2021","journal-title":"Food Chem."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"103696","DOI":"10.1016\/j.jff.2019.103696","article-title":"Milk-derived bioactive peptides exhibit antioxidant activity through the Keap1-Nrf2 signaling pathway","volume":"64","author":"Tonolo","year":"2020","journal-title":"J. Funct. Foods"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"127094","DOI":"10.1016\/j.foodchem.2020.127094","article-title":"The novel peptides ICRD and LCGEC screened from tuna roe show antioxidative activity via Keap1\/Nrf2-ARE pathway regulation and gut microbiota modulation","volume":"327","author":"Han","year":"2020","journal-title":"Food Chem."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"106074","DOI":"10.1016\/j.ultsonch.2022.106074","article-title":"Structural characterization and angiotensin-converting enzyme (ACE) inhibitory mechanism of Stropharia rugosoannulata mushroom peptides prepared by ultrasound","volume":"88","author":"Li","year":"2022","journal-title":"Ultrason Sonochem"},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Ong, J.H., Wong, W.L., Wong, F.C., and Chai, T.T. (2021). Targeting PirAvp and PirBvp Toxins of Vibrio parahaemolyticus with Oilseed Peptides: An In Silico Approach. Antibiotics, 10.","DOI":"10.3390\/antibiotics10101211"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/j.jff.2019.04.050","article-title":"Regulatory requirements of bioactive peptides (protein hydrolysates) from food proteins","volume":"58","author":"Chalamaiah","year":"2019","journal-title":"J. Funct. Foods"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1080\/10915810500259556","article-title":"Studies of the toxicological potential of tripeptides (L-valyl-L-prolyl-L-proline and L-isoleucyl-L-prolyl-L-proline): III. Single- and\/or repeated-dose toxicity of tripeptides-containing Lactobacillus helveticus-fermented milk powder and casein hydrolysate in rats","volume":"24","author":"Maeno","year":"2005","journal-title":"Int. J. Toxicol."},{"key":"ref_62","first-page":"483","article-title":"Food-derived multifunctional bioactive proteins and peptides:Sources and production","volume":"19","author":"Agyei","year":"2015","journal-title":"Biotechnol. Bioact. Compd."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"113162","DOI":"10.1016\/j.lwt.2022.113162","article-title":"Development of antioxidant peptides from brewers\u2019 spent grain proteins","volume":"158","author":"Abeynayake","year":"2022","journal-title":"LWT-Food Sci. Technol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"638","DOI":"10.1016\/j.lwt.2017.11.034","article-title":"Functional characterization and biotechnological potential of exopolysaccharide produced by Lactobacillus rhamnosus strains isolated from human breast milk","volume":"89","author":"Jin","year":"2018","journal-title":"LWT-Food Sci. Technol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"108636","DOI":"10.1016\/j.biopha.2019.108636","article-title":"Polypeptides extracted from Eupolyphaga sinensis walker via enzymic digestion alleviate UV radiation-induced skin photoaging","volume":"112","author":"Zhang","year":"2019","journal-title":"Biomed Pharm."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"1010005","DOI":"10.3389\/fnut.2022.1010005","article-title":"Novel angiotensin-converting enzyme and pancreatic lipase oligopeptide inhibitors from fermented rice bran","volume":"9","author":"Hu","year":"2022","journal-title":"Front. Nutr."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"133551","DOI":"10.1016\/j.foodchem.2022.133551","article-title":"Identification of peptides in Qingke baijiu and evaluation of its angiotensin converting enzyme (ACE) inhibitory activity and stability","volume":"395","author":"Yin","year":"2022","journal-title":"Food Chem."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"763","DOI":"10.5958\/0974-360X.2021.00133.5","article-title":"In silico studies on pancreatic lipase and cholesterol esterase inhibitor 2, 6-di-tert-butyl phenol: A novel molecule for Antiobesity","volume":"2","author":"Pandian","year":"2021","journal-title":"Res. J. Pharm. Technol."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"1949","DOI":"10.1016\/j.peptides.2010.06.020","article-title":"Antioxidative peptides from food proteins: A review","volume":"31","author":"Sarmadi","year":"2010","journal-title":"Peptides"}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/28\/6\/2589\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:53:32Z","timestamp":1760122412000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/28\/6\/2589"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3,13]]},"references-count":69,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2023,3]]}},"alternative-id":["molecules28062589"],"URL":"https:\/\/doi.org\/10.3390\/molecules28062589","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,3,13]]}}}