{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T18:29:11Z","timestamp":1770834551208,"version":"3.50.1"},"reference-count":40,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2023,3,13]],"date-time":"2023-03-13T00:00:00Z","timestamp":1678665600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health (NIH)","doi-asserted-by":"publisher","award":["R01-AT008088"],"award-info":[{"award-number":["R01-AT008088"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health (NIH)","doi-asserted-by":"publisher","award":["T32-AI060546"],"award-info":[{"award-number":["T32-AI060546"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Cripowellins from Crinum erubescens are known pesticidal and have potent antiplasmodial activity. To gain mechanistic insights to this class of natural products, studies to determine the timing of action of cripowellins within the asexual intraerythrocytic cycle of Plasmodium falciparum were performed and led to the observation that this class of natural products induced reversible cytostasis in the ring stage within the first 24 h of treatment. The transcriptional program necessary for P. falciparum to progress through the asexual intraerythrocytic life cycle is well characterized. Whole transcriptome abundance analysis showed that cripowellin B \u201cpauses\u201d the transcriptional program necessary to progress through the intraerythrocytic life cycle coinciding with the lack of morphological progression of drug treated parasites. In addition, cripowellin B-treated parasites re-enter transcriptional progression after treatment was removed. This study highlights the use of cripowellins as chemical probes to reveal new aspects of cell cycle progression of the asexual ring stage of P. falciparum which could be leveraged for the generation of future antimalarial therapeutics.<\/jats:p>","DOI":"10.3390\/molecules28062600","type":"journal-article","created":{"date-parts":[[2023,3,13]],"date-time":"2023-03-13T06:09:56Z","timestamp":1678687796000},"page":"2600","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Cripowellins Pause Plasmodium falciparum Intraerythrocytic Development at the Ring Stage"],"prefix":"10.3390","volume":"28","author":[{"given":"Joshua H.","family":"Butler","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, USA"},{"name":"Center for Tropical and Emerging Global Diseases (CTEGD), University of Georgia, Athens, GA 30602, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heather J.","family":"Painter","sequence":"additional","affiliation":[{"name":"Division of Bacterial, Parasitic, and Allergenic Products, Office of Vaccines Research and Review, Center for Biologics Evaluations and Research, Food and Drug Administration, Silver Spring, MD 20993, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9768-0211","authenticated-orcid":false,"given":"Emily K.","family":"Bremers","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, USA"},{"name":"Center for Tropical and Emerging Global Diseases (CTEGD), University of Georgia, Athens, GA 30602, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Priscilla","family":"Krai","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Virginia Tech, Blacksburg, VA 24060, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6173-5882","authenticated-orcid":false,"given":"Manuel","family":"Llin\u00e1s","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA"},{"name":"Huck Center for Malaria Research, The Pennsylvania State University, University Park, PA 16802, USA"},{"name":"Department of Chemistry, The Pennsylvania State University, University Park, PA 16802, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria B.","family":"Cassera","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, USA"},{"name":"Center for Tropical and Emerging Global Diseases (CTEGD), University of Georgia, Athens, GA 30602, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,13]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (2022). 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