{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T19:09:10Z","timestamp":1777316950574,"version":"3.51.4"},"reference-count":46,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2023,4,10]],"date-time":"2023-04-10T00:00:00Z","timestamp":1681084800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006595","name":"Ministry of Research, Innovation and Digitization, CNCS\u2014UEFISCDI","doi-asserted-by":"publisher","award":["PN-III-P1-1.1-TE-2021-0153"],"award-info":[{"award-number":["PN-III-P1-1.1-TE-2021-0153"]}],"id":[{"id":"10.13039\/501100006595","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Folic acid (vitamin B9) is an essential micronutrient for human health. It can be obtained using different biological pathways as a competitive option for chemical synthesis, but the price of its separation is the key obstacle preventing the implementation of biological methods on a broad scale. Published studies have confirmed that ionic liquids can be used to separate organic compounds. In this article, we investigated folic acid separation by analyzing 5 ionic liquids (CYPHOS IL103, CYPHOS IL104, [HMIM][PF6], [BMIM][PF6], [OMIM][PF6]) and 3 organic solvents (heptane, chloroform, and octanol) as the extraction medium. The best obtained results indicated that ionic liquids are potentially valuable for the recovery of vitamin B9 from diluted aqueous solutions as fermentation broths; the efficiency of the process reached 99.56% for 120 g\/L CYPHOS IL103 dissolved in heptane and pH 4 of the aqueous folic acid solution. Artificial Neural Networks (ANNs) were combined with Grey Wolf Optimizer (GWO) for modelling the process, considering its characteristics.<\/jats:p>","DOI":"10.3390\/molecules28083339","type":"journal-article","created":{"date-parts":[[2023,4,10]],"date-time":"2023-04-10T05:59:33Z","timestamp":1681106373000},"page":"3339","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Folic Acid Ionic-Liquids-Based Separation: Extraction and Modelling"],"prefix":"10.3390","volume":"28","author":[{"given":"Alexandra Cristina","family":"Blaga","sequence":"first","affiliation":[{"name":"\u201cCristofor Simionescu\u201d Faculty of Chemical Engineering and Environmental Protection, \u201cGheorghe Asachi\u201d Technical University of Iasi, D. Mangeron 73, 700050 Iasi, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5006-000X","authenticated-orcid":false,"given":"Elena Niculina","family":"Dragoi","sequence":"additional","affiliation":[{"name":"\u201cCristofor Simionescu\u201d Faculty of Chemical Engineering and Environmental Protection, \u201cGheorghe Asachi\u201d Technical University of Iasi, D. Mangeron 73, 700050 Iasi, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandra","family":"Tucaliuc","sequence":"additional","affiliation":[{"name":"\u201cCristofor Simionescu\u201d Faculty of Chemical Engineering and Environmental Protection, \u201cGheorghe Asachi\u201d Technical University of Iasi, D. Mangeron 73, 700050 Iasi, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lenuta","family":"Kloetzer","sequence":"additional","affiliation":[{"name":"\u201cCristofor Simionescu\u201d Faculty of Chemical Engineering and Environmental Protection, \u201cGheorghe Asachi\u201d Technical University of Iasi, D. Mangeron 73, 700050 Iasi, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6396-0813","authenticated-orcid":false,"given":"Dan","family":"Cascaval","sequence":"additional","affiliation":[{"name":"\u201cCristofor Simionescu\u201d Faculty of Chemical Engineering and Environmental Protection, \u201cGheorghe Asachi\u201d Technical University of Iasi, D. Mangeron 73, 700050 Iasi, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"124624","DOI":"10.1016\/j.biortech.2020.124624","article-title":"Synthetic Biology-Driven Microbial Production of Folates: Advances and Perspectives","volume":"324","author":"Yang","year":"2021","journal-title":"Bioresour. Technol."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Menezo, Y., Elder, K., Clement, A., and Clement, P. (2022). Folic Acid, Folinic Acid, 5 Methyl TetraHydroFolate Supplementation for Mutations That Affect Epigenesis through the Folate and One-Carbon Cycles. 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