{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T13:48:40Z","timestamp":1791035320112,"version":"4.1.0"},"reference-count":45,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2023,4,14]],"date-time":"2023-04-14T00:00:00Z","timestamp":1681430400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Neophytadiene (NPT) is a diterpene found in the methanolic extracts of Crataeva nurvala and Blumea lacera, plants reported with anxiolytic-like activity, sedative properties, and antidepressant-like actions; however, the contribution of neophytadiene to these effects is unknown. This study determined the neuropharmacological (anxiolytic-like, antidepressant-like, anticonvulsant, and sedative) effects of neophytadiene (0.1\u201310 mg\/kg p.o.) and determined the mechanisms of action involved in the neuropharmacological actions using inhibitors such as flumazenil and analyzing the possible interaction of neophytadiene with GABA receptors using a molecular docking study. The behavioral tests were evaluated using the light\u2013dark box, elevated plus-maze, open field, hole-board, convulsion, tail suspension, pentobarbital-induced sleeping, and rotarod. The results showed that neophytadiene exhibited anxiolytic-like activity only to the high dose (10 mg\/kg) in the elevated plus-maze and hole-board tests, and anticonvulsant actions in the 4-aminopyridine and pentylenetetrazole-induced seizures test. The anxiolytic-like and anticonvulsant effects of neophytadiene were abolished with the pre-treatment with 2 mg\/kg flumazenil. In addition, neophytadiene showed low antidepressant effects (about 3-fold lower) compared to fluoxetine. On other hand, neophytadiene had no sedative or locomotor effects. In conclusion, neophytadiene exerts anxiolytic-like and anticonvulsant activities with the probable participation of the GABAergic system.<\/jats:p>","DOI":"10.3390\/molecules28083457","type":"journal-article","created":{"date-parts":[[2023,4,14]],"date-time":"2023-04-14T02:03:38Z","timestamp":1681437818000},"page":"3457","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":104,"title":["In Vivo Neuropharmacological Effects of Neophytadiene"],"prefix":"10.3390","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7573-9294","authenticated-orcid":false,"given":"Maria L.","family":"Gonzalez-Rivera","sequence":"first","affiliation":[{"name":"Departamento de Farmacia, Divisi\u00f3n de Ciencias Naturales y Exactas, Universidad de Guanajuato, Guanajuato 36200, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8860-118X","authenticated-orcid":false,"given":"Juan Carlos","family":"Barragan-Galvez","sequence":"additional","affiliation":[{"name":"Departamento de Farmacia, Divisi\u00f3n de Ciencias Naturales y Exactas, Universidad de Guanajuato, Guanajuato 36200, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5794-7383","authenticated-orcid":false,"given":"Deisy","family":"Gasca-Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Unidad de An\u00e1lisis Conductual, Instituto de Neurobiolog\u00eda, Campus UNAM-Juriquilla, Juriquilla 76230, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4344-642X","authenticated-orcid":false,"given":"Sergio","family":"Hidalgo-Figueroa","sequence":"additional","affiliation":[{"name":"CONACyT-Divisi\u00f3n de Biolog\u00eda Molecular, Instituto Potosino de Investigaci\u00f3n Cient\u00edfica y Tecnol\u00f3gica A.C., San Luis Potos\u00ed 78216, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8389-6866","authenticated-orcid":false,"given":"Mario","family":"Isiordia-Espinoza","sequence":"additional","affiliation":[{"name":"Instituto de Investigaci\u00f3n en Ciencias M\u00e9dicas, Departamento de Cl\u00ednicas, Divisi\u00f3n de Ciencias Biom\u00e9dicas, Centro Universitario de los Altos, Universidad de Guadalajara, Tepatitl\u00e1n de Morelos 47620, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2660-8156","authenticated-orcid":false,"given":"Angel Josabad","family":"Alonso-Castro","sequence":"additional","affiliation":[{"name":"Departamento de Farmacia, Divisi\u00f3n de Ciencias Naturales y Exactas, Universidad de Guanajuato, Guanajuato 36200, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1159\/000521586","article-title":"The Evolution of Neuroepidemiology: Marking the 40-Year Anniversary of Publishing Studies on Epidemiology of Neurological Disorders","volume":"56","author":"Feigin","year":"2022","journal-title":"Neuroepidemiology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"3178","DOI":"10.3389\/fphar.2022.950457","article-title":"Potential therapeutics against neurological disorders: Natural products-based drugs","volume":"13","author":"Rauf","year":"2022","journal-title":"Front. 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