{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:58:33Z","timestamp":1787029113968,"version":"build-2736575974"},"reference-count":30,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2023,4,20]],"date-time":"2023-04-20T00:00:00Z","timestamp":1681948800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"NSFC grants","award":["82173688"],"award-info":[{"award-number":["82173688"]}]},{"name":"NSFC grants","award":["CX20210342"],"award-info":[{"award-number":["CX20210342"]}]},{"name":"NSFC grants","award":["2021zzts0331"],"award-info":[{"award-number":["2021zzts0331"]}]},{"DOI":"10.13039\/501100010083","name":"Hunan Provincial Innovation Foundation for Postgraduate","doi-asserted-by":"publisher","award":["82173688"],"award-info":[{"award-number":["82173688"]}],"id":[{"id":"10.13039\/501100010083","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010083","name":"Hunan Provincial Innovation Foundation for Postgraduate","doi-asserted-by":"publisher","award":["CX20210342"],"award-info":[{"award-number":["CX20210342"]}],"id":[{"id":"10.13039\/501100010083","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010083","name":"Hunan Provincial Innovation Foundation for Postgraduate","doi-asserted-by":"publisher","award":["2021zzts0331"],"award-info":[{"award-number":["2021zzts0331"]}],"id":[{"id":"10.13039\/501100010083","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Fundamental Research Funds for the Central Universities of Central South University (CSU)","award":["82173688"],"award-info":[{"award-number":["82173688"]}]},{"name":"Fundamental Research Funds for the Central Universities of Central South University (CSU)","award":["CX20210342"],"award-info":[{"award-number":["CX20210342"]}]},{"name":"Fundamental Research Funds for the Central Universities of Central South University (CSU)","award":["2021zzts0331"],"award-info":[{"award-number":["2021zzts0331"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Lobophorins (LOBs) are a growing family of spirotetronate natural products with significant cytotoxicity, anti-inflammatory, and antibacterial activities. Herein, we report the transwell-based discovery of Streptomyces sp. CB09030 from a panel of 16 in-house Streptomyces strains, which has significant anti-mycobacterial activity and produces LOB A (1), LOB B (2), and LOB H8 (3). Genome sequencing and bioinformatic analyses revealed the potential biosynthetic gene cluster (BGC) for 1\u20133, which is highly homologous with the reported BGCs for LOBs. However, the glycosyltransferase LobG1 in S. sp. CB09030 has certain point mutations compared to the reported LobG1. Finally, LOB analogue 4 (O-\u03b2-D-kijanosyl-(1\u219217)-kijanolide) was obtained through an acid-catalyzed hydrolysis of 2. Compounds 1\u20134 showed different antibacterial activities against Mycobacterium smegmatis and Bacillus subtilis, which revealed the varying roles of different sugars in their antibacterial activities.<\/jats:p>","DOI":"10.3390\/molecules28083597","type":"journal-article","created":{"date-parts":[[2023,4,20]],"date-time":"2023-04-20T05:35:46Z","timestamp":1681968946000},"page":"3597","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["The Isolation and Structure Elucidation of Spirotetronate Lobophorins A, B, and H8 from Streptomyces sp. CB09030 and Their Biosynthetic Gene Cluster"],"prefix":"10.3390","volume":"28","author":[{"given":"Jie","family":"Shi","sequence":"first","affiliation":[{"name":"Xiangya International Academy of Translational Medicine, Central South University, Changsha 410013, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dian","family":"Peng","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences, Changsha Health Vocational College, Changsha 410100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei-Fei","family":"Peng","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences, Changsha Health Vocational College, Changsha 410100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing-Bo","family":"Zhang","sequence":"additional","affiliation":[{"name":"South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan-Wen","family":"Duan","sequence":"additional","affiliation":[{"name":"Xiangya International Academy of Translational Medicine, Central South University, Changsha 410013, China"},{"name":"Hunan Engineering Research Center of Combinatorial Biosynthesis and Natural Product Drug Discovery, Changsha 410011, China"},{"name":"National Engineering Research Center of Combinatorial Biosynthesis for Drug Discovery, Changsha 410011, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3163-1716","authenticated-orcid":false,"given":"Yong","family":"Huang","sequence":"additional","affiliation":[{"name":"Xiangya International Academy of Translational Medicine, Central South University, Changsha 410013, China"},{"name":"National Engineering Research Center of Combinatorial Biosynthesis for Drug Discovery, Changsha 410011, China"},{"name":"Institute of Health and Medicine, Hefei Comprehensive National Science Center, Hefei 230093, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"9054","DOI":"10.1021\/jo901826d","article-title":"Isolation, structure elucidation, and antitumor activity of spirohexenolides A and B","volume":"74","author":"Kang","year":"2009","journal-title":"J. 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