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Guiyang","award":["MYRG2022-00189-ICMS"],"award-info":[{"award-number":["MYRG2022-00189-ICMS"]}]},{"name":"Scientific and Technologic Project of Guiyang","award":["82260801"],"award-info":[{"award-number":["82260801"]}]},{"name":"Scientific and Technologic Project of Guiyang","award":["82100623"],"award-info":[{"award-number":["82100623"]}]},{"name":"Scientific and Technologic Project of Guiyang","award":["U1812403-4-4"],"award-info":[{"award-number":["U1812403-4-4"]}]},{"name":"Scientific and Technologic Project of Guiyang","award":["gzwkj2023-136"],"award-info":[{"award-number":["gzwkj2023-136"]}]},{"name":"Scientific and Technologic Project of Guiyang","award":["2020-16-9"],"award-info":[{"award-number":["2020-16-9"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Rheumatoid arthritis (RA) is a chronic autoimmune disease triggered by a cascading inflammatory response. Sigesbeckia Herba (SH) has long been utilized as a traditional remedy to alleviate symptoms associated with rheumatism. Our previous study found that leocarpinolide B (LB), a sesquiterpene lactone isolated from the whole plant of SH, possesses potent a anti-inflammatory effect on macrophages. This study was designed to evaluate the therapeutic effects of LB on RA, and further investigate the underlying mechanisms. In collagen type II-induced arthritic mice, LB was demonstrated to decrease the production of autoimmune antibodies in serum and inflammatory cytokines in the joint muscles and recover the decreased regulatory T lymphocytes in spleen. Moreover, LB significantly suppressed the inflammatory infiltration, formation of pannus and bone erosion in the paw joints. In vitro testing showed that LB inhibited the proliferation, migration, invasion, and secretion of inflammatory cytokines in IL-1\u03b2-induced human synovial SW982 cells. Network pharmacology and molecular docking suggested NF-\u03baB p65 could be the potential target of LB on RA treatment, subsequent experimental investigation confirmed that LB directly interacted with NF-\u03baB p65 and reduced the DNA binding activity of NF-\u03baB in synovial cells. In conclusion, LB significantly attenuated the collagen type II-induced arthritis, which was at least involved in the inhibition of DNA binding activity of NF-\u03baB through a direct binding to NF-\u03baB p65. These findings suggest that LB could be a valuable lead compound for developing anti-RA drugs.<\/jats:p>","DOI":"10.3390\/molecules28104241","type":"journal-article","created":{"date-parts":[[2023,5,23]],"date-time":"2023-05-23T02:02:27Z","timestamp":1684807347000},"page":"4241","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Leocarpinolide B Attenuates Collagen Type II-Induced Arthritis by Inhibiting DNA Binding Activity of NF-\u03baB"],"prefix":"10.3390","volume":"28","author":[{"given":"Ke-Gang","family":"Linghu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao SAR, China"},{"name":"The Key Laboratory of Optimal Utilization of Natural Medicine Resources, School of Pharmaceutical Sciences, Guizhou Medical University, Guiyang 550025, 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