{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T10:44:05Z","timestamp":1790765045238,"version":"4.1.0"},"reference-count":67,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2023,5,23]],"date-time":"2023-05-23T00:00:00Z","timestamp":1684800000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"PHC Utique 2020\u20132022 Program (Campus France code: 44197ZG &amp; Comit\u00e9 Mixte franco-tunisien pour la coop\u00e9ration universitaire","award":["20G0822"],"award-info":[{"award-number":["20G0822"]}]},{"name":"PHC Utique 2020\u20132022 Program (Campus France code: 44197ZG &amp; Comit\u00e9 Mixte franco-tunisien pour la coop\u00e9ration universitaire","award":["2020-BALT-806"],"award-info":[{"award-number":["2020-BALT-806"]}]},{"name":"PHC Utique 2020\u20132022 Program (Campus France code: 44197ZG &amp; Comit\u00e9 Mixte franco-tunisien pour la coop\u00e9ration universitaire","award":["2021-BALT-114"],"award-info":[{"award-number":["2021-BALT-114"]}]},{"name":"University of Lille","award":["20G0822"],"award-info":[{"award-number":["20G0822"]}]},{"name":"University of Lille","award":["2020-BALT-806"],"award-info":[{"award-number":["2020-BALT-806"]}]},{"name":"University of Lille","award":["2021-BALT-114"],"award-info":[{"award-number":["2021-BALT-114"]}]},{"name":"Tunisian Ministry of Higher Education and Scientific Research","award":["20G0822"],"award-info":[{"award-number":["20G0822"]}]},{"name":"Tunisian Ministry of Higher Education and Scientific Research","award":["2020-BALT-806"],"award-info":[{"award-number":["2020-BALT-806"]}]},{"name":"Tunisian Ministry of Higher Education and Scientific Research","award":["2021-BALT-114"],"award-info":[{"award-number":["2021-BALT-114"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>The COVID-19 pandemic, caused by SARS-CoV-2, addressed the lack of specific antiviral drugs against coronaviruses. In this study, bioguided fractionation performed on both ethyl acetate and aqueous sub-extracts of Juncus acutus stems led to identifying luteolin as a highly active antiviral molecule against human coronavirus HCoV-229E. The apolar sub-extract (CH2Cl2) containing phenanthrene derivatives did not show antiviral activity against this coronavirus. Infection tests on Huh-7 cells, expressing or not the cellular protease TMPRSS2, using luciferase reporter virus HCoV-229E-Luc showed that luteolin exhibited a dose-dependent inhibition of infection. Respective IC50 values of 1.77 \u00b5M and 1.95 \u00b5M were determined. Under its glycosylated form (luteolin-7-O-glucoside), luteolin was inactive against HCoV-229E. Time of addition assay showed that utmost anti-HCoV-229E activity of luteolin was achieved when added at the post-inoculation step, indicating that luteolin acts as an inhibitor of the replication step of HCoV-229E. Unfortunately, no obvious antiviral activity for luteolin was found against SARS-CoV-2 and MERS-CoV in this study. In conclusion, luteolin isolated from Juncus acutus is a new inhibitor of alphacoronavirus HCoV-229E.<\/jats:p>","DOI":"10.3390\/molecules28114263","type":"journal-article","created":{"date-parts":[[2023,5,23]],"date-time":"2023-05-23T03:20:06Z","timestamp":1684812006000},"page":"4263","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Luteolin Isolated from Juncus acutus L., a Potential Remedy for Human Coronavirus 229E"],"prefix":"10.3390","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3284-6804","authenticated-orcid":false,"given":"Asma","family":"Hakem","sequence":"first","affiliation":[{"name":"Joint Research Unit 1158, BioEcoAgro, Univ. Lille, INRAE, Univ. Li\u00e8ge, UPJV, JUNIA, Univ. Artois, Univ. 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Li\u00e8ge, UPJV, JUNIA, Univ. Artois, Univ. Littoral C\u00f4te d\u2019Opale, ICV\u2014Institut Charles Viollette, 59650 Villeneuve\u2013d\u2019Ascq, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0929-6453","authenticated-orcid":false,"given":"Sophie","family":"Moureu","sequence":"additional","affiliation":[{"name":"Joint Research Unit 1158, BioEcoAgro, Univ. Lille, INRAE, Univ. Li\u00e8ge, UPJV, JUNIA, Univ. Artois, Univ. Littoral C\u00f4te d\u2019Opale, ICV\u2014Institut Charles Viollette, 59650 Villeneuve\u2013d\u2019Ascq, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sevser","family":"Sahpaz","sequence":"additional","affiliation":[{"name":"Joint Research Unit 1158, BioEcoAgro, Univ. Lille, INRAE, Univ. Li\u00e8ge, UPJV, JUNIA, Univ. Artois, Univ. 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