{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T09:08:59Z","timestamp":1785920939450,"version":"3.56.0"},"reference-count":21,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2023,6,2]],"date-time":"2023-06-02T00:00:00Z","timestamp":1685664000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Centre de Recherche du CHUM (internal funding)","award":["N\/A"],"award-info":[{"award-number":["N\/A"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>[13N]Ammonia is one of the most commonly used Positron Emission Tomography (PET) radiotracers in humans to assess myocardial perfusion and measure myocardial blood flow. Here, we report a reliable semi-automated process to manufacture large quantities of [13N]ammonia in high purity by proton-irradiation of a 10 mM aqueous ethanol solution using an in-target process under aseptic conditions. Our simplified production system is based on two syringe driver units and an in-line anion-exchange purification for up to three consecutive productions of ~30 GBq (~800 mCi) (radiochemical yield = 69 \u00b1 3% n.d.c) per day. The total manufacturing time, including purification, sterile filtration, reformulation, and quality control (QC) analyses performed before batch release, is approximately 11 min from the End of Bombardment (EOB). The drug product complies with FDA\/USP specifications and is supplied in a multidose vial allowing for two doses per patient, two patients per batch (4 doses\/batch) on two separate PET scanners simultaneously. After four years of use, this production system has proved to be easy to operate and maintain at low costs. Over the last four years, more than 1000 patients have been imaged using this simplified procedure, demonstrating its reliability for the routine production of large quantities of current Good Manufacturing Practices (cGMP)-compliant [13N]ammonia for human use.<\/jats:p>","DOI":"10.3390\/molecules28114517","type":"journal-article","created":{"date-parts":[[2023,6,2]],"date-time":"2023-06-02T08:50:31Z","timestamp":1685695831000},"page":"4517","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["A Reliable Production System of Large Quantities of [13N]Ammonia for Multiple Human Injections"],"prefix":"10.3390","volume":"28","author":[{"given":"Luis Michel","family":"Alonso Martinez","sequence":"first","affiliation":[{"name":"Radiochemistry and Cyclotron Platform, Centre de Recherche du Centre Hospitalier de l\u2019Universit\u00e9 de Montr\u00e9al (CRCHUM), 900 Rue Saint Denis, Montr\u00e9al, QC H2X 0A9, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nabil","family":"Naim","sequence":"additional","affiliation":[{"name":"Radiochemistry and Cyclotron Platform, Centre de Recherche du Centre Hospitalier de l\u2019Universit\u00e9 de Montr\u00e9al (CRCHUM), 900 Rue Saint Denis, Montr\u00e9al, QC H2X 0A9, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandro Hernandez","family":"Saiz","sequence":"additional","affiliation":[{"name":"Radiochemistry and Cyclotron Platform, Centre de Recherche du Centre Hospitalier de l\u2019Universit\u00e9 de Montr\u00e9al (CRCHUM), 900 Rue Saint Denis, Montr\u00e9al, QC H2X 0A9, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5414-8858","authenticated-orcid":false,"given":"Jos\u00e9-Mathieu","family":"Simard","sequence":"additional","affiliation":[{"name":"Radiochemistry and Cyclotron Platform, Centre de Recherche du Centre Hospitalier de l\u2019Universit\u00e9 de Montr\u00e9al (CRCHUM), 900 Rue Saint Denis, Montr\u00e9al, QC H2X 0A9, Canada"},{"name":"Radiopharmaceutical Science Laboratory, CHU de Qu\u00e9bec, 2250 Boul. Henri-Bourassa, Qu\u00e9bec, QC G1J 5B3, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehdi","family":"Boudjemeline","sequence":"additional","affiliation":[{"name":"Radiochemistry and Cyclotron Platform, Centre de Recherche du Centre Hospitalier de l\u2019Universit\u00e9 de Montr\u00e9al (CRCHUM), 900 Rue Saint Denis, Montr\u00e9al, QC H2X 0A9, Canada"},{"name":"Radiopharmaceutical Science Laboratory, CHU de Qu\u00e9bec, 2250 Boul. Henri-Bourassa, Qu\u00e9bec, QC G1J 5B3, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Juneau","sequence":"additional","affiliation":[{"name":"Radiochemistry and Cyclotron Platform, Centre de Recherche du Centre Hospitalier de l\u2019Universit\u00e9 de Montr\u00e9al (CRCHUM), 900 Rue Saint Denis, Montr\u00e9al, QC H2X 0A9, Canada"},{"name":"Department of Radiology, Radio-Oncology and Nuclear Medicine, UdeM, Pavillon Roger-Gaudry S-716, 2900 Boul. \u00c9douard Montpetit, Montr\u00e9al, QC H3C 3J7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean N.","family":"DaSilva","sequence":"additional","affiliation":[{"name":"Radiochemistry and Cyclotron Platform, Centre de Recherche du Centre Hospitalier de l\u2019Universit\u00e9 de Montr\u00e9al (CRCHUM), 900 Rue Saint Denis, Montr\u00e9al, QC H2X 0A9, Canada"},{"name":"Department of Radiology, Radio-Oncology and Nuclear Medicine, UdeM, Pavillon Roger-Gaudry S-716, 2900 Boul. \u00c9douard Montpetit, Montr\u00e9al, QC H3C 3J7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,6,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1599","DOI":"10.2967\/jnumed.121.261989","article-title":"Practical guide for interpreting and reporting cardiac PET measurements of myocardial blood flow: An information statement from the american society of nuclear cardiology, and the society of nuclear medicine and molecular imaging","volume":"62","author":"Bateman","year":"2021","journal-title":"J. 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