{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T02:13:08Z","timestamp":1760148788489,"version":"build-2065373602"},"reference-count":54,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2023,6,2]],"date-time":"2023-06-02T00:00:00Z","timestamp":1685664000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"22da Convocatoria Interna de Proyectos de Investigaci\u00f3n","award":["106\/2\/C\/33\/22","106\/2\/C\/157\/7"],"award-info":[{"award-number":["106\/2\/C\/33\/22","106\/2\/C\/157\/7"]}]},{"name":"Convocatoria Especial de Apoyo a Necesidades Regionales 2022","award":["106\/2\/C\/33\/22","106\/2\/C\/157\/7"],"award-info":[{"award-number":["106\/2\/C\/33\/22","106\/2\/C\/157\/7"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Cancer is a serious health problem due to the complexity of establishing an effective treatment. The purpose of this work was to evaluate the activity of a triazaspirane as a migration and invasion inhibitor in PC3 prostatic tumor cells through a possible negative regulation of the FAK\/Src signal transduction pathway and decreased secretion of metalloproteinases 2 and 9. Molecular docking analysis was performed using Moe 2008.10 software. Migration (wound-healing assay) and invasion (Boyden chamber assay) assays were performed. In addition, the Western blot technique was used to quantify protein expression, and the zymography technique was used to observe the secretion of metalloproteinases. Molecular docking showed interactions in regions of interest of the FAK and Src proteins. Moreover, the biological activity assays demonstrated an inhibitory effect on cell migration and invasion, an important suppression of metalloproteinase secretion, and a decrease in the expression of p-FAK and p-Src proteins in treated PC3 cells. Triazaspirane-type molecules have important inhibitory effects on the mechanisms associated with metastasis in PC3 tumor cells.<\/jats:p>","DOI":"10.3390\/molecules28114524","type":"journal-article","created":{"date-parts":[[2023,6,2]],"date-time":"2023-06-02T08:50:31Z","timestamp":1685695831000},"page":"4524","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["A Triazaspirane Derivative Inhibits Migration and Invasion in PC3 Prostate Cancer Cells"],"prefix":"10.3390","volume":"28","author":[{"given":"Javier de Jes\u00fas","family":"Vasconcelos-Ulloa","sequence":"first","affiliation":[{"name":"Instituto de Ingenier\u00eda, Universidad Aut\u00f3noma de Baja California, Mexicali 21100, Baja California, Mexico"},{"name":"Facultad de Medicina Mexicali, Universidad Aut\u00f3noma de Baja California, Mexicali 21000, Baja California, Mexico"},{"name":"Laboratorio Multidisciplinario de Estudios Metab\u00f3licos y C\u00e1ncer, Universidad Aut\u00f3noma de Baja California, Mexicali 21000, Baja California, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9421-3730","authenticated-orcid":false,"given":"Victor","family":"Garc\u00eda-Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Facultad de Medicina Mexicali, Universidad Aut\u00f3noma de Baja California, Mexicali 21000, Baja California, Mexico"},{"name":"Laboratorio Multidisciplinario de Estudios Metab\u00f3licos y C\u00e1ncer, Universidad Aut\u00f3noma de Baja California, Mexicali 21000, Baja California, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6788-7545","authenticated-orcid":false,"given":"Benjam\u00edn","family":"Valdez-Salas","sequence":"additional","affiliation":[{"name":"Instituto de Ingenier\u00eda, Universidad Aut\u00f3noma de Baja California, Mexicali 21100, Baja California, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Gustavo","family":"V\u00e1zquez-Jim\u00e9nez","sequence":"additional","affiliation":[{"name":"Facultad de Medicina Mexicali, Universidad Aut\u00f3noma de Baja California, Mexicali 21000, Baja California, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ignacio","family":"Rivero-Espejel","sequence":"additional","affiliation":[{"name":"Centro de Graduados e Investigaci\u00f3n en Qu\u00edmica, Instituto Tecnol\u00f3gico de Tijuana, Tijuana 22000, Baja California, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9400-5683","authenticated-orcid":false,"given":"Ra\u00fal","family":"D\u00edaz-Molina","sequence":"additional","affiliation":[{"name":"Facultad de Medicina Mexicali, Universidad Aut\u00f3noma de Baja California, Mexicali 21000, Baja California, Mexico"},{"name":"Laboratorio Multidisciplinario de Estudios Metab\u00f3licos y C\u00e1ncer, Universidad Aut\u00f3noma de Baja California, Mexicali 21000, Baja California, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4960-7551","authenticated-orcid":false,"given":"Octavio","family":"Galindo-Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Facultad de Medicina Mexicali, Universidad Aut\u00f3noma de Baja California, Mexicali 21000, Baja California, Mexico"},{"name":"Laboratorio Multidisciplinario de Estudios Metab\u00f3licos y C\u00e1ncer, Universidad Aut\u00f3noma de Baja California, Mexicali 21000, Baja California, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,6,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"209","DOI":"10.3322\/caac.21660","article-title":"Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries. CA","volume":"71","author":"Sung","year":"2021","journal-title":"Cancer J. Clin."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1615\/CritRevOncog.v18.i1-2.40","article-title":"On the Origin of Cancer Metastasis","volume":"18","author":"Seyfried","year":"2013","journal-title":"Crit. Rev. Oncog."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Nevedomskaya, E., Baumgart, S.J., and Haendler, B. (2018). Recent Advances in Prostate Cancer Treatment and Drug Discovery. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19051359"},{"key":"ref_4","first-page":"798","article-title":"Localized Prostate Cancer: Treatment Options","volume":"97","author":"Brawley","year":"2018","journal-title":"Am. Fam. Physician"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1007\/s000110050270","article-title":"Evaluation of the cutaneous anti-inflammatory activity of azaspiranes","volume":"47","author":"Griswold","year":"1998","journal-title":"Inflamm. Res."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/S0162-3109(97)00003-9","article-title":"Selective immunomodulatory activity of SK&F 106615, a macrophage-targeting antiarthritic compound, on antibody and cellular responses in rats and mice","volume":"37","author":"Badger","year":"1997","journal-title":"Immunopharmacology"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"4470","DOI":"10.1182\/blood-2004-09-3794","article-title":"Azaspirane (N-N-diethyl-8,8-dipropyl-2-azaspiro [4.5] decane-2-propanamine) inhibits human multiple myeloma cell growth in the bone marrow milieu in vitro and in vivo","volume":"105","author":"Hamasaki","year":"2005","journal-title":"Blood"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1038\/sj.bjc.6602637","article-title":"Atiprimod blocks STAT3 phosphorylation and induces apoptosis in multiple myeloma cells","volume":"93","author":"Shishodia","year":"2005","journal-title":"Br. J. 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Oncol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"34296","DOI":"10.1074\/jbc.M114.601104","article-title":"Development of a novel azaspirane that targets the Janus kinase-signal transducer and activator of transcription (STAT) pathway in hepatocellular carcinoma in vitro and in vivo","volume":"289","author":"Mohan","year":"2014","journal-title":"J. Biol. Chem."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"146","DOI":"10.2174\/157340911795677602","article-title":"Molecular Docking: A powerful approach for structure-based drug discovery","volume":"7","author":"Meng","year":"2011","journal-title":"Curr. Comput. Aided Drug Des."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1038\/nrm1549","article-title":"Focal adhesion kinase: In command and control of cell motility","volume":"6","author":"Mitra","year":"2005","journal-title":"Nat. Rev. Mol. Cell. Biol."},{"key":"ref_15","first-page":"83","article-title":"The dual kinase complex FAK-Src as a promising therapeutic target in cancer","volume":"3","author":"Gasent","year":"2010","journal-title":"OncoTargets Ther."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"948","DOI":"10.1200\/JCO.1999.17.3.948","article-title":"Prognostic Significance of Extent of Disease in Bone in Patients with Androgen-Independent Prostate Cancer","volume":"17","author":"Sabbatini","year":"1999","journal-title":"J. Clin. Oncol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1093\/annonc\/mdi122","article-title":"The Significance of Skeletal-Related Events for the Health-Related Quality of Life of Patients with Metastatic Prostate Cancer","volume":"16","author":"Weinfurt","year":"2005","journal-title":"Ann. Oncol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"6399","DOI":"10.21873\/anticanres.11237","article-title":"Effects of Spiro-bisheterocycles on Proliferation and Apoptosis in Human Breast Cancer Cell Lines","volume":"36","author":"Ramdani","year":"2016","journal-title":"Anticancer Res."},{"key":"ref_19","first-page":"4336","article-title":"Response of 9L Tumor Cells In Vitro to Spirohydantoin Mustard","volume":"39","author":"Deen","year":"1979","journal-title":"Cancer Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"91","DOI":"10.2174\/156800907780006922","article-title":"Small Molecule Inhibitors of Stat3 Signaling Pathway","volume":"7","author":"Deng","year":"2007","journal-title":"Curr. Cancer Drug Targets"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1016\/j.bcp.2008.10.018","article-title":"Novel Derivatives of Spirohydantoin Induce Growth Inhibition Followed by Apoptosis in Leukemia Cells","volume":"77","author":"Kavitha","year":"2009","journal-title":"Biochem. Pharmacol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1016\/j.ceb.2006.08.011","article-title":"Integrin-Regulated FAK-Src Signaling in Normal and Cancer Cells","volume":"18","author":"Mitra","year":"2006","journal-title":"Curr. Opin. Cell Biol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"104735","DOI":"10.1016\/j.bioorg.2021.104735","article-title":"An insight into the structure of 5-spiro aromatic derivatives of imidazolidine-2,4-dione, a new group of very potent inhibitors of tumor multidrug resistance in T-lymphoma cells","volume":"109","author":"Kincses","year":"2021","journal-title":"Bioorg. Chem."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/S0169-409X(00)00129-0","article-title":"Experimental and Computational Approaches to Estimate Solubility and Permeability in Drug Discovery and Development Settings","volume":"46","author":"Lipinski","year":"2001","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1158\/2159-8290.CD-21-1059","article-title":"Hallmarks of Cancer: New Dimensions","volume":"12","author":"Hanahan","year":"2022","journal-title":"Cancer Discov."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"100899","DOI":"10.1016\/j.tranon.2020.100899","article-title":"Plasticity of Cancer Cell Invasion: Patterns and Mechanisms","volume":"14","author":"Wu","year":"2021","journal-title":"Transl. Oncol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/S0065-230X(08)00403-X","article-title":"FAK Expression Regulation and Therapeutic Potential","volume":"101","author":"Li","year":"2008","journal-title":"Adv. Cancer Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1186\/s13046-019-1265-1","article-title":"The Roles of Nuclear Focal Adhesion Kinase (FAK) on Cancer: A Focused Review","volume":"38","author":"Zhou","year":"2019","journal-title":"J. Exp. Clin. Cancer Res. CR"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Tapial Mart\u00ednez, P., L\u00f3pez Navajas, P., and Lietha, D. (2020). FAK Structure and Regulation by Membrane Interactions and Force in Focal Adhesions. Biomolecules, 10.","DOI":"10.3390\/biom10020179"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1007\/978-1-4939-7845-8_4","article-title":"Protocols for Migration and Invasion Studies in Prostate Cancer","volume":"1786","author":"Buijs","year":"2018","journal-title":"Methods Mol. Biol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"17","DOI":"10.32607\/20758251-2015-7-2-17-28","article-title":"Cancer Invasion: Patterns and Mechanisms","volume":"7","author":"Krakhmal","year":"2015","journal-title":"Acta Nat."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1539","DOI":"10.1530\/EC-19-0442","article-title":"Leptin Induces Cell Migration and Invasion in a FAK-Src-Dependent Manner in Breast Cancer Cells","volume":"8","year":"2019","journal-title":"Endocr. Connect."},{"key":"ref_33","first-page":"7265","article-title":"Genistein Inhibits Invasion and Migration of Colon Cancer Cells by Recovering WIF1 Expression","volume":"17","author":"Zhu","year":"2018","journal-title":"Mol. Med. Rep."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.gendis.2014.12.002","article-title":"Targeting Matrix Metalloproteinases in Cancer: Bringing New Life to Old Ideas","volume":"2","author":"Cathcart","year":"2015","journal-title":"Genes Dis."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1016\/j.matbio.2015.01.022","article-title":"Matrix Metalloproteinases in Stem Cell Regulation and Cancer","volume":"44\u201346","author":"Kessenbrock","year":"2015","journal-title":"Matrix Biol. J. Int. Soc. Matrix Biol."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Niland, S., Riscanevo, A.X., and Eble, J.A. (2022). Matrix Metalloproteinases Shape the Tumor Microenvironment in Cancer Progression. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23010146"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1158\/2326-6066.CIR-14-0112","article-title":"NF-\u039aB, an Active Player in Human Cancers","volume":"2","author":"Xia","year":"2014","journal-title":"Cancer Immunol. Res."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Pang, X.-J., Liu, X.-J., Liu, Y., Liu, W.-B., Li, Y.-R., Yu, G.-X., Tian, X.-Y., Zhang, Y.-B., Song, J., and Jin, C.-Y. (2021). Drug Discovery Targeting Focal Adhesion Kinase (FAK) as a Promising Cancer Therapy. Molecules, 26.","DOI":"10.3390\/molecules26144250"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"735","DOI":"10.2174\/187152010794728648","article-title":"Focal Adhesion Kinase as a Cancer Therapy Target","volume":"10","author":"Golubovskaya","year":"2010","journal-title":"Anticancer Agents Med. Chem."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1002\/mc.20380","article-title":"TAE226-Induced Apoptosis in Breast Cancer Cells with Overexpressed Src or EGFR","volume":"47","author":"Golubovskaya","year":"2008","journal-title":"Mol. Carcinog."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1935","DOI":"10.1158\/0008-5472.CAN-07-5155","article-title":"Antitumor Activity and Pharmacology of a Selective Focal Adhesion Kinase Inhibitor, PF-562,271","volume":"68","author":"Roberts","year":"2008","journal-title":"Cancer Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1158\/1535-7163.MCT-06-0561","article-title":"Deactivation of Akt and STAT3 Signaling Promotes Apoptosis, Inhibits Proliferation, and Enhances the Sensitivity of Hepatocellular Carcinoma Cells to an Anticancer Agent, Atiprimod","volume":"6","author":"Choudhari","year":"2007","journal-title":"Mol. Cancer Ther."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1016\/j.ctrv.2010.08.003","article-title":"Src-Mediated Regulation of Homotypic Cell Adhesion: Implications for Cancer Progression and Opportunities for Therapeutic Intervention","volume":"37","author":"Wadhawan","year":"2011","journal-title":"Cancer Treat. Rev."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.phrs.2015.01.003","article-title":"Src Protein-Tyrosine Kinase Structure, Mechanism, and Small Molecule Inhibitors","volume":"94","author":"Roskoski","year":"2015","journal-title":"Pharmacol. Res."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"989","DOI":"10.3389\/fonc.2019.00989","article-title":"Cadherin Signaling in Cancer: Its Functions and Role as a Therapeutic Target","volume":"9","author":"Yu","year":"2019","journal-title":"Front. Oncol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"806","DOI":"10.1016\/0955-0674(93)90029-P","article-title":"Cadherins in Cancer: Implications for Invasion and Metastasis","volume":"5","author":"Takeichi","year":"1993","journal-title":"Curr. Opin. Cell Biol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"109320","DOI":"10.1016\/j.biopha.2019.109320","article-title":"Aberrant N-Cadherin Expression in Cancer","volume":"118","author":"Cao","year":"2019","journal-title":"Biomed. Pharmacother."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"835","DOI":"10.1039\/b719215k","article-title":"Cadherins in Development and Cancer","volume":"4","author":"Stemmler","year":"2008","journal-title":"Mol. Biosyst."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/S0304-3835(01)00759-5","article-title":"Cadherins and the Tumour Progression: Is It All in a Switch?","volume":"176","author":"Cavallaro","year":"2002","journal-title":"Cancer Lett."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Kaszak, I., Witkowska-Pi\u0142aszewicz, O., Niewiadomska, Z., Dworecka-Kaszak, B., Ngosa Toka, F., and Jurka, P. (2020). Role of Cadherins in Cancer\u2014A Review. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21207624"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"260","DOI":"10.3998\/ark.5550190.0012.221","article-title":"Microwave-assisted synthesis of cycloalkanespirohydantoins and piperidylspirohydantoins as precursors of restricted \u03b1-amino acids","volume":"2011","author":"Rivero","year":"2011","journal-title":"Arkivoc"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"2019","DOI":"10.3390\/ijms12032019","article-title":"Amyloidogenic Properties of a D\/N Mutated 12 Amino Acid Fragment of the C-Terminal Domain of the Cholesteryl-Ester Transfer Protein (CETP)","volume":"12","year":"2011","journal-title":"Int. J. Mol. Sci."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1016\/j.plefa.2014.09.002","article-title":"Extracellular Vesicles from MDA-MB-231 Breast Cancer Cells Stimulated with Linoleic Acid Promote an EMT-like Process in MCF10A Cells","volume":"91","author":"Salazar","year":"2014","journal-title":"Prostaglandins Leukot. Essent. Fatty Acids"},{"key":"ref_54","unstructured":"Rosner, B. (2006). Fundamentals of Biostatistics, Thomson-Brooks\/Cole. [6th ed.]."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/28\/11\/4524\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:48:06Z","timestamp":1760125686000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/28\/11\/4524"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6,2]]},"references-count":54,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2023,6]]}},"alternative-id":["molecules28114524"],"URL":"https:\/\/doi.org\/10.3390\/molecules28114524","relation":{},"ISSN":["1420-3049"],"issn-type":[{"type":"electronic","value":"1420-3049"}],"subject":[],"published":{"date-parts":[[2023,6,2]]}}}