{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T20:26:40Z","timestamp":1774470400258,"version":"3.50.1"},"reference-count":34,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2023,6,15]],"date-time":"2023-06-15T00:00:00Z","timestamp":1686787200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Jiangxi University of Chinese Medicine Science and Technology Innovation Team","award":["CXTD22007"],"award-info":[{"award-number":["CXTD22007"]}]},{"name":"Jiangxi University of Chinese Medicine Science and Technology Innovation Team","award":["2019YFC1604905"],"award-info":[{"award-number":["2019YFC1604905"]}]},{"name":"Jiangxi University of Chinese Medicine Science and Technology Innovation Team","award":["JXSYLXK-ZHYAO137"],"award-info":[{"award-number":["JXSYLXK-ZHYAO137"]}]},{"name":"Jiangxi University of Chinese Medicine Science and Technology Innovation Team","award":["YC2022 \u2013 S839"],"award-info":[{"award-number":["YC2022 \u2013 S839"]}]},{"name":"National Key Research and Development Program Project","award":["CXTD22007"],"award-info":[{"award-number":["CXTD22007"]}]},{"name":"National Key Research and Development Program Project","award":["2019YFC1604905"],"award-info":[{"award-number":["2019YFC1604905"]}]},{"name":"National Key Research and Development Program Project","award":["JXSYLXK-ZHYAO137"],"award-info":[{"award-number":["JXSYLXK-ZHYAO137"]}]},{"name":"National Key Research and Development Program Project","award":["YC2022 \u2013 S839"],"award-info":[{"award-number":["YC2022 \u2013 S839"]}]},{"name":"Special Scientific Research Fund Project for First-class Discipline of Traditional Chinese Medicine in Jiangxi Province","award":["CXTD22007"],"award-info":[{"award-number":["CXTD22007"]}]},{"name":"Special Scientific Research Fund Project for First-class Discipline of Traditional Chinese Medicine in Jiangxi Province","award":["2019YFC1604905"],"award-info":[{"award-number":["2019YFC1604905"]}]},{"name":"Special Scientific Research Fund Project for First-class Discipline of Traditional Chinese Medicine in Jiangxi Province","award":["JXSYLXK-ZHYAO137"],"award-info":[{"award-number":["JXSYLXK-ZHYAO137"]}]},{"name":"Special Scientific Research Fund Project for First-class Discipline of Traditional Chinese Medicine in Jiangxi Province","award":["YC2022 \u2013 S839"],"award-info":[{"award-number":["YC2022 \u2013 S839"]}]},{"name":"Jiangxi Province Postgraduate Innovation Special Fund Project","award":["CXTD22007"],"award-info":[{"award-number":["CXTD22007"]}]},{"name":"Jiangxi Province Postgraduate Innovation Special Fund Project","award":["2019YFC1604905"],"award-info":[{"award-number":["2019YFC1604905"]}]},{"name":"Jiangxi Province Postgraduate Innovation Special Fund Project","award":["JXSYLXK-ZHYAO137"],"award-info":[{"award-number":["JXSYLXK-ZHYAO137"]}]},{"name":"Jiangxi Province Postgraduate Innovation Special Fund Project","award":["YC2022 \u2013 S839"],"award-info":[{"award-number":["YC2022 \u2013 S839"]}]},{"name":"State Administration of Traditional Chinese Medicine of Jiangxi University of Chinese Medicine Key Discipline Cultivation Discipline of Traditional Chinese Medicine Pharmacology of Traditional Chinese Medicine","award":["CXTD22007"],"award-info":[{"award-number":["CXTD22007"]}]},{"name":"State Administration of Traditional Chinese Medicine of Jiangxi University of Chinese Medicine Key Discipline Cultivation Discipline of Traditional Chinese Medicine Pharmacology of Traditional Chinese Medicine","award":["2019YFC1604905"],"award-info":[{"award-number":["2019YFC1604905"]}]},{"name":"State Administration of Traditional Chinese Medicine of Jiangxi University of Chinese Medicine Key Discipline Cultivation Discipline of Traditional Chinese Medicine Pharmacology of Traditional Chinese Medicine","award":["JXSYLXK-ZHYAO137"],"award-info":[{"award-number":["JXSYLXK-ZHYAO137"]}]},{"name":"State Administration of Traditional Chinese Medicine of Jiangxi University of Chinese Medicine Key Discipline Cultivation Discipline of Traditional Chinese Medicine Pharmacology of Traditional Chinese Medicine","award":["YC2022 \u2013 S839"],"award-info":[{"award-number":["YC2022 \u2013 S839"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Genipin has been the focus of research as a multifunctional compound for the treatment of pathogenic diseases. However, hepatotoxicity caused by oral genipin raises concerns about its safety. To obtain novel derivatives with low toxicity and efficacy, we synthesized methylgenipin (MG), a new compound, using structural modification, and investigated the safety of MG administration. The results showed that the LD50 of oral MG was higher than 1000 mg\/kg, no mice died or were poisoned during the experiment in the treatment group, and there was no significant difference in biochemical parameters and liver pathological sections compared with the control. Importantly, MG (100 mg\/kg\/d) treatment for 7 days reduced alpha-naphthylisothiocyanate (ANIT)-induced increases in liver index, alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (AKP), and total bilirubin (TBIL) levels. Histopathology demonstrated that MG could treat ANIT-induced cholestasis. In addition, using proteomics to investigate the molecular mechanism of MG in the treatment of a liver injury may be related to enhancing antioxidant function. Kit validation showed that ANIT induced an increase in malondialdehyde (MDA) and a decrease in superoxide dismutase (SOD) and glutathione (GSH) levels, while the MG pretreatments, both of which were significantly reversed to some extent, suggested that MG may alleviate ANIT-induced hepatotoxicity by enhancing endogenous antioxidant enzymes and inhibiting oxidative stress injury. In this study, we demonstrate that the treatment of mice with MG does not cause impaired liver function and provide an investigation of the efficacy of MG against ANIT-induced hepatotoxicity, laying the foundation for the safety evaluation and clinical application of MG.<\/jats:p>","DOI":"10.3390\/molecules28124793","type":"journal-article","created":{"date-parts":[[2023,6,16]],"date-time":"2023-06-16T02:02:20Z","timestamp":1686880940000},"page":"4793","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Synthesis of Methylgenipin and Evaluation of Its Anti-Hepatic Injury Activity"],"prefix":"10.3390","volume":"28","author":[{"given":"Jingjing","family":"Wang","sequence":"first","affiliation":[{"name":"School of Pharmacy, Jiangxi University of Chinese Medicine, Nanchang 330004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongwei","family":"Qiu","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Jiangxi University of Chinese Medicine, Nanchang 330004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yaohui","family":"Chen","sequence":"additional","affiliation":[{"name":"Jiangxi Provincial People\u2019s Hospital, Nanchang 330012, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feng","family":"Zhou","sequence":"additional","affiliation":[{"name":"Jiangxi Provincial People\u2019s Hospital, Nanchang 330012, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuaikang","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Jiangxi University of Chinese Medicine, Nanchang 330004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liping","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Jiangxi University of Chinese Medicine, Nanchang 330004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yinfang","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Jiangxi University of Chinese Medicine, Nanchang 330004, China"},{"name":"Jiangxi Provincial Key Laboratory of Pharmacology of Traditional Chinese Medicine, Nanchang 330004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Riyue","family":"Yu","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Jiangxi University of Chinese Medicine, Nanchang 330004, China"},{"name":"Jiangxi Provincial Key Laboratory of Pharmacology of Traditional Chinese Medicine, Nanchang 330004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8120-8342","authenticated-orcid":false,"given":"Liping","family":"Huang","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Jiangxi University of Chinese Medicine, Nanchang 330004, China"},{"name":"Jiangxi Provincial Key Laboratory of Pharmacology of Traditional Chinese Medicine, Nanchang 330004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,6,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/j.jhep.2018.09.014","article-title":"Burden of liver diseases in the world","volume":"70","author":"Asrani","year":"2019","journal-title":"J. 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