{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T18:40:49Z","timestamp":1785523249088,"version":"3.56.0"},"reference-count":67,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2023,6,28]],"date-time":"2023-06-28T00:00:00Z","timestamp":1687910400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004488","name":"Croatian Science Foundation","doi-asserted-by":"publisher","award":["IPCH-2020-10-4965"],"award-info":[{"award-number":["IPCH-2020-10-4965"]}],"id":[{"id":"10.13039\/501100004488","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Considering the biological and ecological importance of Cu\u2013thiol interactions and the discrepancies in previous research, this study focuses on Cu interactions with biologically and ecologically relevant thiols: glutathione (GSH), L-cysteine (L-cys), 3-mercaptopropionic acid (MPA), and thioacetic acid (TAA) in aqueous solution. The addition of Cu(II) to a thiol-containing solution led to a rapid reduction of Cu(II) and the formation of a Cu(I)\u2013thiol complex. The mechanism of Cu(II) reduction and Cu(I) complex formation as well as the kinetics of Cu(I) oxidation strongly depend on the structural properties of the individual thiols investigated. The reducing power of the investigated thiols can be summarized as follows: L-cys \u2245 GSH &gt; MPA &gt; TAA. The reaction order, with respect to Cu(I) oxidation, also changes over the time of the reaction course. The deviation of the reaction kinetics from the first order with respect to Cu(I) in the later stages of the reaction course can be attributed to a Fenton-like reaction occurring under low thiol concentration conditions. At high Cu:thiol ratios, in the case of GSH, L-cys, and MPA, the early stage of the reaction course is characterized by high Cu(I) stability, most likely as a result of Cu(I) complexation by the thiols present in excess in the reaction mixture.<\/jats:p>","DOI":"10.3390\/molecules28135065","type":"journal-article","created":{"date-parts":[[2023,6,29]],"date-time":"2023-06-29T01:43:13Z","timestamp":1688002993000},"page":"5065","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Interaction between Cu and Thiols of Biological and Environmental Importance: Case Study Using Combined Spectrophotometric\/Bathocuproine Sulfonate Disodium Salt Hydrate (BCS) Assay"],"prefix":"10.3390","volume":"28","author":[{"given":"Dora","family":"Crmari\u0107","sequence":"first","affiliation":[{"name":"Division for Marine and Environmental Research, Ru\u0111er Bo\u0161kovi\u0107 Institute, Bijeni\u010dka Cesta 54, 10 000 Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elvira","family":"Bura-Naki\u0107","sequence":"additional","affiliation":[{"name":"Division for Marine and Environmental Research, Ru\u0111er Bo\u0161kovi\u0107 Institute, Bijeni\u010dka Cesta 54, 10 000 Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,6,28]]},"reference":[{"key":"ref_1","first-page":"1","article-title":"Introduction and overview of copper as an element essential for life","volume":"Volume 10","author":"Frieden","year":"1991","journal-title":"Biochemistry of Copper"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1007\/978-94-007-5561-1_12","article-title":"The copper metallome in prokaryotic cells","volume":"12","author":"Rensing","year":"2013","journal-title":"Met. 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