{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T17:04:30Z","timestamp":1782234270859,"version":"3.54.5"},"reference-count":54,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2023,7,15]],"date-time":"2023-07-15T00:00:00Z","timestamp":1689379200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Science and Technology Development Fund","award":["SKL-QRCM(UM)-2020-2022"],"award-info":[{"award-number":["SKL-QRCM(UM)-2020-2022"]}]},{"name":"Science and Technology Development Fund","award":["SKL-QRCM-OP21005"],"award-info":[{"award-number":["SKL-QRCM-OP21005"]}]},{"name":"State Key Laboratory of Quality Research in Chinese Medicine, University of Macau","award":["SKL-QRCM(UM)-2020-2022"],"award-info":[{"award-number":["SKL-QRCM(UM)-2020-2022"]}]},{"name":"State Key Laboratory of Quality Research in Chinese Medicine, University of Macau","award":["SKL-QRCM-OP21005"],"award-info":[{"award-number":["SKL-QRCM-OP21005"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>A biomimetic mineralization method was used in the facile and rapid preparation of nanoflowers for immobilizing alcohol dehydrogenase (ADH). The method mainly uses ADH as an organic component and zinc phosphate as an inorganic component to prepare flower-like ADH\/Zn3(PO4)2 organic-inorganic hybrid nanoflowers (HNFs) with the high specific surface area through a self-assembly process. The synthesis conditions of the ADH HNFs were optimized and its morphology was characterized. Under the optimum enzymatic reaction conditions, the Michaelis-Menten constant (Km) of ADH HNFs (\u03b2-NAD+ as substrate) was measured to be 3.54 mM, and the half-maximal inhibitory concentration (IC50) of the positive control ranitidine (0.2\u20130.8 mM) was determined to be 0.49 mM. Subsequently, the inhibitory activity of natural medicine Penthorum chinense Pursh and nine small-molecule compounds on ADH was evaluated using ADH HNFs. The inhibition percentage of the aqueous extract of P.\u00a0chinense is 57.9%. The vanillic acid, protocatechuic acid, gallic acid, and naringenin have obvious inhibitory effects on ADH, and their percentages of inhibition are 55.1%, 68.3%, 61.9%, and 75.5%, respectively. Moreover, molecular docking analysis was applied to explore the binding modes and sites of the four most active small-molecule compounds to ADH. The results of this study can broaden the application of immobilized enzymes through biomimetic mineralization, and provide a reference for the discovery of ADH inhibitors from natural products.<\/jats:p>","DOI":"10.3390\/molecules28145429","type":"journal-article","created":{"date-parts":[[2023,7,17]],"date-time":"2023-07-17T00:56:47Z","timestamp":1689555407000},"page":"5429","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Preparation of Alcohol Dehydrogenase\u2013Zinc Phosphate Hybrid Nanoflowers through Biomimetic Mineralization and Its Application in the Inhibitor Screening"],"prefix":"10.3390","volume":"28","author":[{"given":"Mao-Ling","family":"Luo","sequence":"first","affiliation":[{"name":"School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guo-Ying","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengpeng","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4355-6490","authenticated-orcid":false,"given":"Yitao","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9300-5710","authenticated-orcid":false,"given":"Feng-Qing","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,7,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Wolszczak-Biedrzycka, B., Zasimowicz-Majewska, E., Bie\u0144kowska, A., Biedrzycki, G., Dorf, J., and Jelski, W. 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