{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T10:58:13Z","timestamp":1790420293735,"version":"4.1.0"},"reference-count":56,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2023,7,17]],"date-time":"2023-07-17T00:00:00Z","timestamp":1689552000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"University Grants Commission, SAP Scheme","award":["F.3-23\/2007-SAP II"],"award-info":[{"award-number":["F.3-23\/2007-SAP II"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Malaria remains a life-threatening health problem and is responsible for the high rates of mortality and morbidity in the tropical and subtropical regions of the world. The increasing threat of drug resistance to available artemisinin-based therapy warrants an urgent need to develop new antimalarial drugs that are safer, more effective, and have a novel mode of action. Natural plants are an excellent source of inspiration in searching for a new antimalarial agent. This research reports a systematic investigation for determining the antimalarial potential of the seeds of A. squamosa. The study shows that the crude seed extract (CSE), protein, saponin, and the oily fractions of the seeds were nontoxic at a 2000 mg\/kg body weight dose when tested in Wistar rats, thus revealing high safety is classified as class 5. The oily fraction, Annomaal, demonstrated pronounced antimalarial activity with low IC50 (1.25 \u00b1 0.183 \u03bcg\/mL) against P. falciparum in vitro. The CSE and Annomaal significantly inhibited the growth of P. berghei parasites in vivo with 58.47% and 61.11% chemo suppression, respectively, while the standard drug artemether showed chemo suppression of 66.75%. Furthermore, the study demonstrated that oral administration of Annomaal at a daily dose of 250 mg\/kg\/day for 3 days was adequate to provide a complete cure to the P. berghei-infected mice. Annomaal thus holds promise as being patient-compliant due to the shorter treatment schedule, eliminating the need for frequent dosing for extended time periods as required by several synthetic antimalarial drugs. Further studies are needed to determine the active compounds in the oily fraction responsible for antimalarial activity.<\/jats:p>","DOI":"10.3390\/molecules28145472","type":"journal-article","created":{"date-parts":[[2023,7,18]],"date-time":"2023-07-18T01:46:02Z","timestamp":1689644762000},"page":"5472","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["In Vitro Effect on Plasmodium falciparum and In Vivo Effect on Plasmodium berghei of Annomaal, an Oily Fraction Obtained from the Seeds of Annona squamosa"],"prefix":"10.3390","volume":"28","author":[{"given":"Sampada S.","family":"Sawant","sequence":"first","affiliation":[{"name":"C.U. Shah College of Pharmacy, SNDT Women\u2019s University, Santacruz (West), Mumbai 400049, India"},{"name":"Cipla Limited, Mumbai 400013, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satish Y.","family":"Gabhe","sequence":"additional","affiliation":[{"name":"C.U. Shah College of Pharmacy, SNDT Women\u2019s University, Santacruz (West), Mumbai 400049, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7325-0711","authenticated-orcid":false,"given":"Kamalinder K.","family":"Singh","sequence":"additional","affiliation":[{"name":"C.U. Shah College of Pharmacy, SNDT Women\u2019s University, Santacruz (West), Mumbai 400049, India"},{"name":"School of Pharmacy and Biomedical Sciences, University of Central Lancashire, Preston PR1 2HE, UK"},{"name":"Biomedical Evidence Based Transdisciplinary Health Research Institute, University of Central Lancashire, Preston PR1 2HE, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,7,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Cox, F.E.G. (2010). History of the discovery of the malaria parasites and their vectors. Parasites Vectors, 3.","DOI":"10.1186\/1756-3305-3-5"},{"key":"ref_2","unstructured":"WHO (2022). Guidelines for Malaria, World Health Organization. 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