{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T04:35:27Z","timestamp":1789792527693,"version":"4.0.1"},"reference-count":55,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2023,7,20]],"date-time":"2023-07-20T00:00:00Z","timestamp":1689811200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Universal CNPQ project","doi-asserted-by":"publisher","award":["432458\/2018.2"],"award-info":[{"award-number":["432458\/2018.2"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"name":"PROPESP\/UFPA","award":["432458\/2018.2"],"award-info":[{"award-number":["432458\/2018.2"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>From Eleutherine plicata, naphthoquinones, isoeleutherine, and eleutherol were isolated, and previous studies have reported the antioxidant activity of these metabolites. The present work evaluated the role of oxidative changes in mice infected with Plasmodium berghei and treated with E. plicata extract, fraction, and isolated compounds, as well as to verify possible oxidative changes induced by these treatments. E. plicata extracts were prepared from powder from the bulbs, which were submitted to maceration with ethanol, yielding the extract (EEEp), which was fractionated under reflux, and the dichloromethane fraction (FDMEp) was submitted for further fractionation, leading to the isolation of isoeleutherine, eleutherine, and eleutherol. The antimalarial activity was examined using the suppressive test, evaluating the following parameters of oxidative stress: trolox equivalent antioxidant capacity (TEAC), thiobarbituric acid reactive substances (TBARS), and reduced glutathione (GSH). Furthermore, the molecular docking of naphthoquinones, eleutherol, eleutherine, and isoeleutherine interactions with antioxidant defense enzymes was investigated, which was favorable for the formation of the receptor\u2013ligand complex, according to the re-rank score values. Eleutherine and isoeleutherine are the ones with the lowest binding energy for catalase (CAT), glutathione reductase (GR), and glutathione peroxidase (GPx1), showing themselves as possible targets of these molecules in the involvement of redox balance. Data from the present study showed that treatments with E. plicata stimulated an increase in antioxidant capacity and a reduction in oxidative stress in mice infected with P. berghei, with naphthoquinones being responsible for reducing oxidative changes and disease severity.<\/jats:p>","DOI":"10.3390\/molecules28145557","type":"journal-article","created":{"date-parts":[[2023,7,21]],"date-time":"2023-07-21T01:58:38Z","timestamp":1689904718000},"page":"5557","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Participation of Oxidative Stress in the Activity of Compounds Isolated from Eleutherine plicata Herb"],"prefix":"10.3390","volume":"28","author":[{"given":"Ant\u00f4nio Rafael Quadros","family":"Gomes","sequence":"first","affiliation":[{"name":"Postgraduate Program in Pharmaceutical Innovation, Institute of Health Sciences, Federal University of Para, Belem 66075-110, PA, Brazil"},{"name":"Oxidative Stress Research Lab, Institute of Biological Sciences, Federal University of Para, Belem 66075-110, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0529-3714","authenticated-orcid":false,"given":"Jorddy Neves","family":"Cruz","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Pharmaceutical Sciences, Institute of Health Sciences, Federal University of Para, Belem 66075-110, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Laura Gadelha","family":"Castro","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Pharmaceutical Innovation, Institute of Health Sciences, Federal University of Para, Belem 66075-110, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8472-2179","authenticated-orcid":false,"given":"Heliton Patrick","family":"Cordovil Brigido","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Pharmaceutical Innovation, Institute of Health Sciences, Federal University of Para, Belem 66075-110, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9710-3791","authenticated-orcid":false,"given":"Everton Luiz Pompeu","family":"Varela","sequence":"additional","affiliation":[{"name":"Oxidative Stress Research Lab, Institute of Biological Sciences, Federal University of Para, Belem 66075-110, PA, Brazil"},{"name":"Postgraduate Program in Biodiversity and Biotechnology of the BIONORTE Network, Federal University of Para, Belem 66075-110, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valdicley Vieira","family":"Vale","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Pharmaceutical Innovation, Institute of Health Sciences, Federal University of Para, Belem 66075-110, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9097-2385","authenticated-orcid":false,"given":"Liliane Almeida","family":"Carneiro","sequence":"additional","affiliation":[{"name":"Evandro Chagas Institute, Belem 66093-020, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3682-7945","authenticated-orcid":false,"given":"Gleison Gon\u00e7alves","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Pharmaceutical Sciences, Institute of Health Sciences, Federal University of Para, Belem 66075-110, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9528-0361","authenticated-orcid":false,"given":"Sandro","family":"Percario","sequence":"additional","affiliation":[{"name":"Oxidative Stress Research Lab, Institute of Biological Sciences, Federal University of Para, Belem 66075-110, PA, Brazil"},{"name":"Postgraduate Program in Biodiversity and Biotechnology of the BIONORTE Network, Federal University of Para, Belem 66075-110, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0804-5804","authenticated-orcid":false,"given":"Maria F\u00e2ni","family":"Dolabela","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Pharmaceutical Innovation, Institute of Health Sciences, Federal University of Para, Belem 66075-110, PA, Brazil"},{"name":"Postgraduate Program in Pharmaceutical Sciences, Institute of Health Sciences, Federal University of Para, Belem 66075-110, PA, Brazil"},{"name":"Postgraduate Program in Biodiversity and Biotechnology of the BIONORTE Network, Federal University of Para, Belem 66075-110, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,7,20]]},"reference":[{"key":"ref_1","unstructured":"WHO (2021). 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