{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,28]],"date-time":"2026-06-28T05:42:26Z","timestamp":1782625346107,"version":"3.54.5"},"reference-count":53,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2023,7,30]],"date-time":"2023-07-30T00:00:00Z","timestamp":1690675200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Instituto Polit\u00e9cnico Nacional","award":["SIP 20230132"],"award-info":[{"award-number":["SIP 20230132"]}]},{"name":"CONACYT","award":["SIP 20230132"],"award-info":[{"award-number":["SIP 20230132"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Among antihyperglycemic drugs used for treating diabetes, \u03b1-glucosidase inhibitors generate the least adverse effects. This contribution aimed to evaluate the potential antidiabetic activity of Rumex crispus L. by testing its in vitro \u03b1-glucosidase inhibition and in vivo antihyperglycemic effects on rats with streptozotocin (STZ)-induced diabetes. Better inhibition of \u03b1-glucosidase was found with the methanol extract versus the n-hexane and dichloromethane extracts. The methanol extract of the flowers (RCFM) was more effective than that of the leaves (RCHM), with an IC50 of 7.3 \u00b1 0.17 \u03bcg\/mL for RCFM and 112.0 \u00b1 1.23 \u03bcg\/mL for RCHM. A bioactive fraction (F89s) also showed good \u03b1-glucosidase inhibition (IC50 = 3.8 \u00b1 0.11 \u03bcg\/mL). In a preliminary study, RCHM and RCFM at 150 mg\/kg and F89s at 75 mg\/kg after 30 days showed a significant effect on hyperglycemia, reducing glucose levels (82.2, 80.1, and 84.1%, respectively), and improved the lipid, renal, and hepatic profiles of the rats, comparable with the effects of metformin and acarbose. According to the results, the activity of R. crispus L. may be mediated by a diminished rate of disaccharide hydrolysis, associated with the inhibition of \u03b1-glucosidase. Thus, R. crispus L. holds promise for the development of auxiliary drugs to treat diabetes mellitus.<\/jats:p>","DOI":"10.3390\/molecules28155760","type":"journal-article","created":{"date-parts":[[2023,7,31]],"date-time":"2023-07-31T02:13:32Z","timestamp":1690769612000},"page":"5760","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Evaluation of \u03b1-Glucosidase Inhibition and Antihyperglycemic Activity of Extracts Obtained from Leaves and Flowers of Rumex crispus L."],"prefix":"10.3390","volume":"28","author":[{"given":"Dolores G.","family":"Aguila-Mu\u00f1oz","sequence":"first","affiliation":[{"name":"Centro de Investigaci\u00f3n en Biotecnolog\u00eda Aplicada, Instituto Polit\u00e9cnico Nacional, Tlaxcala 90700, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0776-1705","authenticated-orcid":false,"given":"Fabiola E.","family":"Jim\u00e9nez-Montejo","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Biotecnolog\u00eda Aplicada, Instituto Polit\u00e9cnico Nacional, Tlaxcala 90700, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1885-2901","authenticated-orcid":false,"given":"V\u00edctor E.","family":"L\u00f3pez-L\u00f3pez","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Biotecnolog\u00eda Aplicada, Instituto Polit\u00e9cnico Nacional, Tlaxcala 90700, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aar\u00f3n","family":"Mendieta-Moctezuma","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Biotecnolog\u00eda Aplicada, Instituto Polit\u00e9cnico Nacional, Tlaxcala 90700, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge","family":"Rodr\u00edguez-Antol\u00edn","sequence":"additional","affiliation":[{"name":"Centro Tlaxcala de Biolog\u00eda de la Conducta, Universidad Aut\u00f3noma de Tlaxcala, Tlaxcala 90070, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge","family":"Cornejo-Garrido","sequence":"additional","affiliation":[{"name":"Laboratorio de Biolog\u00eda Celular y Productos Naturales, Escuela Nacional de Medicina y Homeopat\u00eda, Instituto Polit\u00e9cnico Nacional, Ciudad de M\u00e9xico 07320, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5815-0587","authenticated-orcid":false,"given":"Mar\u00eda C.","family":"Cruz-L\u00f3pez","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Biotecnolog\u00eda Aplicada, Instituto Polit\u00e9cnico Nacional, Tlaxcala 90700, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,7,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"110383","DOI":"10.1016\/j.foodres.2021.110383","article-title":"Antidiabetic Potential of Dietary Polyphenols: A Mechanistic Review","volume":"145","author":"Pastore","year":"2021","journal-title":"Food Res. Int."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1216","DOI":"10.1080\/14756366.2017.1368503","article-title":"\u03b1-Glucosidase Inhibition by Flavonoids: An in Vitro and in Silico Structure\u2013Activity Relationship Study","volume":"32","author":"Freitas","year":"2017","journal-title":"J. Enzyme Inhib. Med. Chem."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"111738","DOI":"10.1016\/j.fct.2020.111738","article-title":"An Overview on the Role of Bioactive \u03b1-Glucosidase Inhibitors in Ameliorating Diabetic Complications","volume":"145","author":"Hossain","year":"2020","journal-title":"Food Chem. Toxicol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"107843","DOI":"10.1016\/j.diabres.2019.107843","article-title":"Global and Regional Diabetes Prevalence Estimates for 2019 and Projections for 2030 and 2045: Results from the International Diabetes Federation Diabetes Atlas, 9th Edition","volume":"157","author":"Saeedi","year":"2019","journal-title":"Diabetes Res. Clin. Pract."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"100230","DOI":"10.1016\/j.hermed.2018.09.001","article-title":"Diabetes Mellitus and Nature\u2019s Pharmacy of Putative Antidiabetic Plants","volume":"15","author":"Chinsembu","year":"2019","journal-title":"J. Herb. Med."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1016\/j.jtcme.2017.08.012","article-title":"An Update on Natural Compounds in the Remedy of Diabetes Mellitus: A Systematic Review","volume":"8","author":"Choudhury","year":"2018","journal-title":"J. Tradit. Complement. Med."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1016\/j.jaim.2016.11.002","article-title":"Antidiabetic, Antihyperlipidemic Activities and Herb\u2013Drug Interaction of a Polyherbal Formulation in Streptozotocin Induced Diabetic Rats","volume":"8","author":"Choudhari","year":"2017","journal-title":"J. Ayurveda Integr. Med."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Salehi, B., Ata, A., Anil Kumar, N.V., Sharopov, F., Ram\u00edrez-Alarc\u00f3n, K., Ruiz-Ortega, A., Ayatollahi, S.A., Fokou, P.V.T., Kobarfard, F., and Zakaria, Z.A. (2019). Antidiabetic Potential of Medicinal Plants and Their Active Components. Biomolecules, 9.","DOI":"10.3390\/biom9100551"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1016\/j.bioorg.2019.02.009","article-title":"The Management of Diabetes Mellitus-Imperative Role of Natural Products against Dipeptidyl Peptidase-4, \u03b1-Glucosidase and Sodium-Dependent Glucose Co-Transporter 2 (SGLT2)","volume":"86","author":"Abbas","year":"2019","journal-title":"Bioorg. Chem."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"128119","DOI":"10.1016\/j.foodchem.2020.128119","article-title":"Fruit, Vegetables, and Mushrooms for the Preparation of Extracts with \u03b1-Amylase and \u03b1-Glucosidase Inhibition Properties: A Review","volume":"338","author":"Papoutsis","year":"2021","journal-title":"Food Chem."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"112336","DOI":"10.1016\/j.biopha.2021.112336","article-title":"Dietary Natural Products as a Potential Inhibitor towards Advanced Glycation End Products and Hyperglycemic Complications: A Phytotherapy Approaches","volume":"144","author":"Vijaykrishnaraj","year":"2021","journal-title":"Biomed. Pharmacother."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.ajps.2017.08.004","article-title":"Bioactive Flavonoids in Medicinal Plants: Structure, Activity and Biological Fate","volume":"13","author":"Wang","year":"2018","journal-title":"Asian J. Pharm. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"129056","DOI":"10.1016\/j.foodchem.2021.129056","article-title":"Comparative Study of Inhibition Mechanisms of Structurally Different Flavonoid Compounds on \u03b1-Glucosidase and Synergistic Effect with Acarbose","volume":"347","author":"Yang","year":"2021","journal-title":"Food Chem."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"27","DOI":"10.14715\/cmb\/2018.64.8.5","article-title":"Bioactive Compounds and Health Benefits of Edible Rumex Species\u2013A Review","volume":"64","author":"Mishra","year":"2018","journal-title":"Cell. Mol. Biol."},{"key":"ref_15","first-page":"148","article-title":"Investigation of genus Rumex for their biologically active constituents","volume":"2","author":"Shafiq","year":"2017","journal-title":"Res. J. Life Sci. Bioinforatics Pharm. Chem. Sci."},{"key":"ref_16","unstructured":"Mart\u00ednez, R.M.M. (2005). Flora Fanerog\u00e1mica Del Valle de M\u00e9xico, Comisi\u00f3n Nacional para el Conocimiento y Uso de la Biodiversidad. [1st ed.]."},{"key":"ref_17","first-page":"20","article-title":"Rumex crispus L.\u2014A Plant of Traditional Value","volume":"9","author":"Pareek","year":"2014","journal-title":"Drug Discov."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1007\/s13659-022-00346-z","article-title":"The Genus Rumex (Polygonaceae): An Ethnobotanical, Phytochemical and Pharmacological Review","volume":"12","author":"Li","year":"2022","journal-title":"Nat. Products Bioprospect."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1016\/j.jep.2015.09.001","article-title":"The Genus Rumex: Review of Traditional Uses, Phytochemistry and Pharmacology","volume":"175","author":"Vasas","year":"2015","journal-title":"J. Ethnopharmacol."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Buzgaia, N., Awin, T., Elabbar, F., Abdusalam, K., Lee, S.Y., Rukayadi, Y., Abas, F., and Shaari, K. (2020). Antibacterial Activity of Arbutus Pavarii Pamp against Methicillin-Resistant Staphylococcus Aureus (MRSA) and UHPLC-MS\/MS Profile of the Bioactive Fraction. Plants, 9.","DOI":"10.3390\/plants9111539"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"534","DOI":"10.1016\/j.foodchem.2018.06.056","article-title":"UPLC-Orbitrap-MS\/MS Combined with Chemometrics Establishes Variations in Chemical Components in Green Tea from Yunnan and Hunan Origins","volume":"266","author":"Xin","year":"2018","journal-title":"Food Chem."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"679","DOI":"10.3390\/12030679","article-title":"Screening Non-Colored Phenolics in Red Wines Using Liquid Chromatography\/Ultraviolet and Mass Spectrometry\/Mass Spectrometry Libraries","volume":"12","author":"Sun","year":"2007","journal-title":"Molecules"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"3353","DOI":"10.1039\/C4AY00064A","article-title":"The Screening of Potential \u03b1-Glucosidase Inhibitors from the Polygonum Multiflorum Extract Using Ultrafiltration Combined with Liquid Chromatography-Tandem Mass Spectrometry","volume":"6","author":"Yang","year":"2014","journal-title":"Anal. Methods"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1016\/j.jpba.2007.11.014","article-title":"Analysis of the Constituents in the Rat Plasma after Oral Administration of Yin Chen Hao Tang by UPLC\/Q-TOF-MS\/MS","volume":"46","author":"Wang","year":"2008","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/j.jpba.2014.01.046","article-title":"HPLC-DAD-ESI\/MS n Analysis of Phenolic Compounds for Quality Control of Grindelia Robusta Nutt. and Bioactivities","volume":"94","author":"Ferreres","year":"2014","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.jpba.2018.09.001","article-title":"Use of Ultra-High-Performance Liquid Chromatography Coupled with Quadrupole-Time-of-Flight Mass Spectrometry System as Valuable Tool for an Untargeted Metabolomic Profiling of Rumex tunetanus Flowers and Stems and Contribution to the Antioxidant Activity","volume":"162","author":"Abidi","year":"2019","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.jpba.2015.08.034","article-title":"Rapid Identification and Quantitative Analysis of the Chemical Constituents in Scutellaria indica L. by UHPLC-QTOF-MS and UHPLC-MS\/MS","volume":"117","author":"He","year":"2016","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Zolkeflee, N.K.Z., Ramli, N.S., Azlan, A., and Abas, F. (2022). In Vitro Anti-Diabetic Activities and UHPLC-ESI-MS\/MS Profile of Muntingia Calabura Leaves Extract. Molecules, 27.","DOI":"10.3390\/molecules27010287"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1016\/j.talanta.2012.11.072","article-title":"Identification of Phenolic Compounds in Equisetum Giganteum by LC\u2013ESI-MS\/MS and a New Approach to Total Flavonoid Quantification","volume":"105","author":"Francescato","year":"2013","journal-title":"Talanta"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Farag, M.A., Kabbash, E.M., Mediani, A., D\u00f6ll, S., Esatbeyoglu, T., and Afifi, S.M. (2022). Comparative Metabolite Fingerprinting of Four Different Cinnamon Species Analyzed via UPLC\u2013MS and GC\u2013MS and Chemometric Tools. Molecules, 27.","DOI":"10.3390\/molecules27092935"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Feduraev, P.V., Chupakhina, G., Maslennikov, P., Tacenko, N., and Skrypnik, L. (2019). Variation in Phenolic Compounds Content and Antioxidant Activity of Different Plant Organs from Rumex crispus L. and Rumex obtusifolius L. at Different Growth Stages. Antioxidants, 8.","DOI":"10.3390\/antiox8070237"},{"key":"ref_32","first-page":"123","article-title":"The Accumulation Features of Phenolic Compounds in Some Species of Genus Rumex L.","volume":"3","author":"Feduraev","year":"2017","journal-title":"Khimiya Rastit. Syr\u2019ya"},{"key":"ref_33","first-page":"962","article-title":"Phytochemical and Antioxidant Activities of Rumex crispus L. in Treatment of Gastrointestinal Helminths in Eastern Cape Province, South Africa","volume":"6","author":"Idris","year":"2016","journal-title":"Asian Pac. J. Trop. Biomed."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1186\/1749-8546-8-19","article-title":"Flavonoids with \u03b1-Glucosidase Inhibitory Activities and Their Contents in the Leaves of Morus Atropurpurea","volume":"8","author":"Hong","year":"2013","journal-title":"Chin. Med."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1186\/s13065-018-0445-y","article-title":"Inhibitory Effects against \u03b1-Glucosidase and \u03b1-Amylase of the Flavonoids-Rich Extract from Scutellaria Baicalensis Shoots and Interpretation of Structure\u2013Activity Relationship of Its Eight Flavonoids by a Refined Assign-Score Method","volume":"12","author":"Li","year":"2018","journal-title":"Chem. Cent. J."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"111081","DOI":"10.1016\/j.lwt.2021.111081","article-title":"Synergistic Inhibition of Isolated Flavonoids from Moringa Oleifera Leaf on \u03b1-Glucosidase Activity","volume":"141","author":"Hamed","year":"2021","journal-title":"LWT"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Chokki, M., Cudalbeanu, M., Zongo, C., Dah-Nouvlessounon, D., Ghinea, I.O., Furdui, B., Raclea, R., Savadogo, A., Baba-Moussa, L., and Avamescu, S.M. (2020). Exploring Antioxidant and Enzymes (A-Amylase and B-Glucosidase) Inhibitory Activity of Morinda Lucida and Momordica Charantia Leaves from Benin. Foods, 9.","DOI":"10.3390\/foods9040434"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"108727","DOI":"10.1016\/j.lwt.2019.108727","article-title":"Inhibition of \u03b1-Amylase, \u03b1-Glucosidase and Pancreatic Lipase by Phenolic Compounds of Rumex maderensis (Madeira sorrel). Influence of Simulated Gastrointestinal Digestion on Hyperglycaemia-Related Damage Linked with Aldose Reductase Activity and Protein G","volume":"118","author":"Castilho","year":"2020","journal-title":"LWT Food Sci. Technol."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Minh, T.N., Van, T.M., Andriana, Y., Vinh, L.T., Hau, D.V., Duyen, D.H., and de Guzman-Gelani, C. (2019). Antioxidant, Xanthine Oxidase, \u03b1-Amylase and \u03b1-Glucosidase Inhibitory Activities of Bioactive Compounds from Rumex crispus L. Root. Molecules, 24.","DOI":"10.3390\/molecules24213899"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"3252","DOI":"10.1039\/C6FO00695G","article-title":"Phenolic Compounds and Biological Effects of Edible: Rumex scutatus and Pseudosempervivum sempervivum: Potential Sources of Natural Agents with Health Benefits","volume":"7","author":"Savran","year":"2016","journal-title":"Food Funct."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"111202","DOI":"10.1016\/j.fct.2020.111202","article-title":"Rumex dentatus L. Phenolics Ameliorate Hyperglycemia by Modulating Hepatic Key Enzymes of Carbohydrate Metabolism, Oxidative Stress and PPAR\u03b3 in Diabetic Rats","volume":"138","author":"Elsayed","year":"2020","journal-title":"Food Chem. Toxicol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/j.pathophys.2010.03.001","article-title":"Antihyperglycemic and Antihyperlipidemic Effect of Rumex patientia Seed Preparation in Streptozotocin-Diabetic Rats","volume":"18","author":"Sedaghat","year":"2011","journal-title":"Pathophysiology"},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Sampietro, D.A., Catalan, C.A.N., Vattuone, M.A., and Narwal, S.S. (2009). Isolation, Identification and Characterization of Allelochemicals\/Natural Products, Taylor & Francis Ltd.,.","DOI":"10.1201\/b10195"},{"key":"ref_44","first-page":"375","article-title":"Antioxidant Activity of Four Color Fractions of Bee Pollen from M\u00e9rida, Venezuela","volume":"62","author":"Vit","year":"2012","journal-title":"Arch. Latinoam. Nutr."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"15","DOI":"10.17795\/jjnpp-7621","article-title":"Total Phenolic, Total Flavonoids, Antioxidant and Antimicrobial Activities of Scrophularia Striata Boiss Extracts","volume":"8","author":"Mahboubi","year":"2013","journal-title":"Jundishapur J. Nat. Pharm. Prod."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"2133","DOI":"10.1002\/jsfa.6538","article-title":"High-Throughput Micro-Plate HCl-Vanillin Assay for Screening Tannin Content in Sorghum Grain","volume":"94","author":"Herald","year":"2014","journal-title":"J. Sci. Food Agric."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Makkar, H.P.S., Siddhuraju, P., and Becker, K. (2007). Plant Secondary Metabolites, Humana Press. Methods in Molecular BiologyTM.","DOI":"10.1007\/978-1-59745-425-4"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1007\/s11745-006-3013-5","article-title":"The Liebermann\u2013Burchard Reaction: Sulfonation, Desaturation, and Rearrangment of Cholesterol in Acid","volume":"42","author":"Xiong","year":"2007","journal-title":"Lipids"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"198","DOI":"10.4103\/0257-7941.107361","article-title":"Estimation of Total Alkaloid in Chitrakadivati by UV-Spectrophotometer","volume":"31","author":"Ajanal","year":"2012","journal-title":"Anc. Sci. Life"},{"key":"ref_50","first-page":"257","article-title":"\u03b1-Glucosidase and \u03b1-Amylase Inhibitory Effect and Antioxidant Activity of Ten Plant Extracts Traditionally Used in Iran for Diabetes","volume":"7","author":"Salehi","year":"2013","journal-title":"J. Med. Plants Res."},{"key":"ref_51","unstructured":"(1999). Especificaciones T\u00e9cnicas Para La Producci\u00f3n, Cuidado y Uso de Los Animales de Laboratorio (Standard No. Norma Oficial Mexicana NOM-062-ZOO-1999)."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"e78","DOI":"10.1002\/cpz1.78","article-title":"Streptozotocin-Induced Diabetic Models in Mice and Rats","volume":"1","author":"Furman","year":"2021","journal-title":"Curr. Protoc."},{"key":"ref_53","unstructured":"(1995). Sacrificio Humanitario de Los Animales Dom\u00e9sticos y Silvestres (Standard No. Norma Oficial Mexicana NOM-033-ZOO-1995)."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/28\/15\/5760\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:22:37Z","timestamp":1760127757000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/28\/15\/5760"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,7,30]]},"references-count":53,"journal-issue":{"issue":"15","published-online":{"date-parts":[[2023,8]]}},"alternative-id":["molecules28155760"],"URL":"https:\/\/doi.org\/10.3390\/molecules28155760","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,7,30]]}}}