{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T01:19:42Z","timestamp":1775179182602,"version":"3.50.1"},"reference-count":51,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2023,8,2]],"date-time":"2023-08-02T00:00:00Z","timestamp":1690934400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFD1600401"],"award-info":[{"award-number":["2021YFD1600401"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["CARS-20"],"award-info":[{"award-number":["CARS-20"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["JJKH20210328KJ"],"award-info":[{"award-number":["JJKH20210328KJ"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"name":"China Agriculture Research System","award":["2021YFD1600401"],"award-info":[{"award-number":["2021YFD1600401"]}]},{"name":"China Agriculture Research System","award":["CARS-20"],"award-info":[{"award-number":["CARS-20"]}]},{"name":"China Agriculture Research System","award":["JJKH20210328KJ"],"award-info":[{"award-number":["JJKH20210328KJ"]}]},{"name":"Science and Technology Program of Jilin Educational Committee","award":["2021YFD1600401"],"award-info":[{"award-number":["2021YFD1600401"]}]},{"name":"Science and Technology Program of Jilin Educational Committee","award":["CARS-20"],"award-info":[{"award-number":["CARS-20"]}]},{"name":"Science and Technology Program of Jilin Educational Committee","award":["JJKH20210328KJ"],"award-info":[{"award-number":["JJKH20210328KJ"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Flammulina rossica fermentation extract (FREP) was obtained by ethanol precipitation of the fermentation broth. The molecular weight of FREP is 28.52 kDa, and it mainly contains active ingredients such as polysaccharides, proteins, reducing sugars, and 16 amino acids. Among them, the polysaccharides were mannose, glucose, galactose, arabinose, and fucose and possessed \u03b2-glycosidic bonds. Furthermore, the immunoregulatory activities of FREP were investigated in vivo. The results demonstrated that FREP could increase the counts of CD4+ T lymphocytes and the ratio of CD4+\/CD8+ in a dose-dependent manner in healthy mice. In addition, FREP significantly increased serum cytokines, including IL-2, IL-8, IL-10, IL-12, IL-6, IL-1\u03b2, INF-\u03b3, C-rection protein, and TNF-\u03b1, and promoted splenocyte proliferation in healthy mice. Finally, FREP could restore the counts of white blood cells, red blood cells, secretory immunoglobulin A, and antibody-forming cells and significantly promote the serum haemolysin level in mice treated with cyclophosphamide. The findings indicated that FREP possessed immunoregulatory activity in healthy mice and could improve the immune functions in immunosuppressive mice. Therefore, FREP could be exploited as an immunomodulatory agent and potential immunotherapeutic medicine for patients with inadequate immune function.<\/jats:p>","DOI":"10.3390\/molecules28155825","type":"journal-article","created":{"date-parts":[[2023,8,2]],"date-time":"2023-08-02T11:17:17Z","timestamp":1690975037000},"page":"5825","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Immunomodulatory Effect of Flammulina rossica Fermentation Extract on Healthy and Immunosuppressed Mice"],"prefix":"10.3390","volume":"28","author":[{"given":"Yingdi","family":"Dai","sequence":"first","affiliation":[{"name":"Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130118, China"},{"name":"College of Plant Protection, Jilin Agricultural University, Changchun 130012, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sijia","family":"Ma","sequence":"additional","affiliation":[{"name":"Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130118, China"},{"name":"College of Plant Protection, Jilin Agricultural University, Changchun 130012, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanyan","family":"Zhu","sequence":"additional","affiliation":[{"name":"Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130118, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2918-730X","authenticated-orcid":false,"given":"Andrey A.","family":"Gontcharov","sequence":"additional","affiliation":[{"name":"Institute of Biology and Soil Science, FEB RAS, 100-Letia Vladivostoka Prospect, 159, Vladivostok 690022, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Liu","sequence":"additional","affiliation":[{"name":"Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130118, China"},{"name":"College of Plant Protection, Jilin Agricultural University, Changchun 130012, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qi","family":"Wang","sequence":"additional","affiliation":[{"name":"Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130118, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1581","DOI":"10.1021\/jf4053012","article-title":"A Novel Polysaccharide from Ganoderma atrum Exerts Antitumor Activity by Activating Mitochondria-Mediated Apoptotic Pathway and Boosting the Immune System","volume":"62","author":"Zhang","year":"2014","journal-title":"J. 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