{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T19:33:26Z","timestamp":1791401606270,"version":"4.3.3"},"reference-count":37,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2023,8,4]],"date-time":"2023-08-04T00:00:00Z","timestamp":1691107200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["U19A2034"],"award-info":[{"award-number":["U19A2034"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["GXXT-2019-49"],"award-info":[{"award-number":["GXXT-2019-49"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["CARS-19"],"award-info":[{"award-number":["CARS-19"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"key grant for the University Synergy Innovation Program of Anhui Province","award":["U19A2034"],"award-info":[{"award-number":["U19A2034"]}]},{"name":"key grant for the University Synergy Innovation Program of Anhui Province","award":["GXXT-2019-49"],"award-info":[{"award-number":["GXXT-2019-49"]}]},{"name":"key grant for the University Synergy Innovation Program of Anhui Province","award":["CARS-19"],"award-info":[{"award-number":["CARS-19"]}]},{"name":"China Agriculture Research System of MOF and MARA","award":["U19A2034"],"award-info":[{"award-number":["U19A2034"]}]},{"name":"China Agriculture Research System of MOF and MARA","award":["GXXT-2019-49"],"award-info":[{"award-number":["GXXT-2019-49"]}]},{"name":"China Agriculture Research System of MOF and MARA","award":["CARS-19"],"award-info":[{"award-number":["CARS-19"]}]},{"name":"Department of Sciences and Technology of Anhui Province","award":["U19A2034"],"award-info":[{"award-number":["U19A2034"]}]},{"name":"Department of Sciences and Technology of Anhui Province","award":["GXXT-2019-49"],"award-info":[{"award-number":["GXXT-2019-49"]}]},{"name":"Department of Sciences and Technology of Anhui Province","award":["CARS-19"],"award-info":[{"award-number":["CARS-19"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Theaflavins (TFs), the primary bioactive components in black tea, are poorly absorbed in the small intestine. However, the biological activity of TFs does not match their low bioavailability, which suggests that the gut microbiota plays a crucial role in their biotransformation and activities. In this study, we aimed to investigate the biotransferred metabolites of TFs produced by the human gut microbiota and these metabolites\u2019 function. We profiled the microbial metabolites of TFs by in vitro anaerobic human gut microbiota fermentation using liquid chromatography tandem mass spectrometry (LC-MS\/MS) methods. A total of 17 microbial metabolites were identified, and their corresponding metabolic pathways were proposed. Moreover, full-length 16S rRNA gene sequence analysis revealed that the TFs altered the gut microbiota diversity and increased the relative abundance of specific members of the microbiota involved in the catabolism of the TFs, including Flavonifractor_plautii, Bacteroides_uniformis, Eubacterium_ramulus, etc. Notably, the antioxidant capacity of the TF sample increased after fermentation compared to the initial sample. In conclusion, the results contribute to a more comprehensive understanding of the microbial metabolites and antioxidant capacity of TFs.<\/jats:p>","DOI":"10.3390\/molecules28155871","type":"journal-article","created":{"date-parts":[[2023,8,4]],"date-time":"2023-08-04T09:28:29Z","timestamp":1691141309000},"page":"5871","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Microbial-Transferred Metabolites of Black Tea Theaflavins by Human Gut Microbiota and Their Impact on Antioxidant Capacity"],"prefix":"10.3390","volume":"28","author":[{"given":"Li","family":"Sun","sequence":"first","affiliation":[{"name":"State Key Laboratory of Tea Plant Biology and Utilization, School of Tea and Food Sciences and Technology, Anhui Agricultural University, Hefei 230036, China"},{"name":"The College of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"You","family":"Su","sequence":"additional","affiliation":[{"name":"The College of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaiyin","family":"Hu","sequence":"additional","affiliation":[{"name":"The College of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daxiang","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tea Plant Biology and Utilization, School of Tea and Food Sciences and Technology, Anhui Agricultural University, Hefei 230036, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huimin","family":"Guo","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tea Plant Biology and Utilization, School of Tea and Food Sciences and Technology, Anhui Agricultural University, Hefei 230036, China"},{"name":"Center for Biotechnology, Anhui Agricultural University, Hefei 230036, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3080-4881","authenticated-orcid":false,"given":"Zhongwen","family":"Xie","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tea Plant Biology and Utilization, School of Tea and Food Sciences and Technology, Anhui Agricultural University, Hefei 230036, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e2000887","DOI":"10.1002\/mnfr.202000887","article-title":"Black Tea Theaflavin Detoxifies Metabolic Toxins in the Intestinal Tract of Mice","volume":"65","author":"Zhang","year":"2021","journal-title":"Mol. 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