{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T22:43:25Z","timestamp":1790808205598,"version":"4.1.0"},"reference-count":58,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2023,8,25]],"date-time":"2023-08-25T00:00:00Z","timestamp":1692921600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Rutabaga, also known as swede and scientifically classified as Brassica napus napobrassica, is a biennial edible root vegetable that belongs to the Brassica genus and is widely cultivated in North Europe and North America. The present study highlights both the phytochemical profile and the in vitro biological properties of rutabaga seed extracts obtained through maceration using solvents of increasing polarity, namely, cyclohexane (CYHA), dichloromethane (DCM), ethyl acetate (EtOAc), methanol (MeOH), and water (H2O). HPLC-DAD was used to identify and quantify phenolic compounds, while volatile compounds were detected using GC-MS. The in vitro antioxidant capacity of the rutabaga seed extracts was evaluated through DPPH free radical scavenging activity. The in vitro anti-inflammatory activity (15-lipoxygenase (15-LOX) enzyme) was determined spectrophotometrically at the same concentration. Additionally, the cytotoxicity of the seed extracts was evaluated against human colon adenocarcinoma cells (Caco-2) and human embryonic kidney cells (HEK-293) using the MTT assay. The rutabaga seed extracts obtained from EtOAc, MeOH, and H2O were particularly rich in reducing sugars, ranging from 189.87 to 473.75 mg\/g DW. The MeOH extract displayed the highest concentration of both sugars and polyphenols. Phytochemically, the HPLC-DAD analysis revealed the presence of four phenolic compounds in the tested extracts, including (\u00b1) synephrine, gallic acid, p-coumaric acid, and trans-ferulic acid, newly discovered in rutabaga organs. Moreover, a total of ten volatile compounds were identified through GC-MS analysis, both before and after derivatization. At a concentration of 50 \u00b5g\/mL, the methanol extract exhibited high antioxidant activity with 52.95% inhibition, while CYHA, DCM, and EtOAc exhibited moderate anti-15-LOX activity with less than 30% inhibition. Except for DCM and aqueous extracts, rutabaga seeds did not exhibit any anti-proliferative potential against Caco-2 cell lines. Interestingly, no cytotoxicity was registered for any of the seed extracts against the normal cell line HEK-293. Overall, the obtained data highlight the potential utilization of rutabaga seeds as a source of bioactive compounds in various fields, including pharmaceuticals, nutraceuticals, and functional foods.<\/jats:p>","DOI":"10.3390\/molecules28176250","type":"journal-article","created":{"date-parts":[[2023,8,25]],"date-time":"2023-08-25T09:09:00Z","timestamp":1692954540000},"page":"6250","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["The Phytochemical Screening and Biological Properties of Brassica napus L. var. napobrassica (Rutabaga) Seeds"],"prefix":"10.3390","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5949-7419","authenticated-orcid":false,"given":"Jawaher","family":"Ayadi","sequence":"first","affiliation":[{"name":"Laboratoire de Recherche, Biodiversit\u00e9, Mol\u00e9cule et Application, Institut Sup\u00e9rieur de Biologie Appliqu\u00e9e de M\u00e9denine, Universit\u00e9 de Gab\u00e8s, Zrig, Gab\u00e8s 6072, Tunisia"},{"name":"Laboratoire de G\u00e9nie Chimique, Universit\u00e9 de Toulouse, CNRS, INPT, UPS, F-31062 Toulouse, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6473-6143","authenticated-orcid":false,"given":"Mohamed","family":"Debouba","sequence":"additional","affiliation":[{"name":"Laboratoire de Recherche, Biodiversit\u00e9, Mol\u00e9cule et Application, Institut Sup\u00e9rieur de Biologie Appliqu\u00e9e de M\u00e9denine, Universit\u00e9 de Gab\u00e8s, Zrig, Gab\u00e8s 6072, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7686-7597","authenticated-orcid":false,"given":"Rami","family":"Rahmani","sequence":"additional","affiliation":[{"name":"Laboratoire de Recherche, Biodiversit\u00e9, Mol\u00e9cule et Application, Institut Sup\u00e9rieur de Biologie Appliqu\u00e9e de M\u00e9denine, Universit\u00e9 de Gab\u00e8s, Zrig, Gab\u00e8s 6072, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2439-5145","authenticated-orcid":false,"given":"Jalloul","family":"Bouajila","sequence":"additional","affiliation":[{"name":"Laboratoire de G\u00e9nie Chimique, Universit\u00e9 de Toulouse, CNRS, INPT, UPS, F-31062 Toulouse, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"4660","DOI":"10.1002\/ptr.7099","article-title":"Natural Products: A Lead for Drug Discovery and Development","volume":"35","author":"Chopra","year":"2021","journal-title":"Phytother. 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