{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T15:54:10Z","timestamp":1790610850835,"version":"4.1.0"},"reference-count":43,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2023,8,30]],"date-time":"2023-08-30T00:00:00Z","timestamp":1693353600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the National Natural Science Foundation of China","award":["No. 21375209"],"award-info":[{"award-number":["No. 21375209"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Coptisine (Cop) exerts a neuroprotective effect on central nervous system disease, particularly ischemic stroke. However, its protective mechanism is still unclear. This study aimed to investigate the protective effect of Cop on cerebral ischemia\u2013reperfusion (IR) rats with a middle cerebral artery occlusion model by integrating a gas chromatography\u2013mass spectrometry (GC\u2013MS)-based metabolomics approach with biochemical assessment. Our results showed that Cop could improve neurobehavioral function and decrease the ischemia size in IR rats. In addition, Cop was found to decrease inflammatory mediators (e.g., prostaglandin D2 (PGD2) and tumor necrosis factor-\u03b1 (TNF-\u03b1) and attenuate oxidative stress response (e.g., increase the superoxide dismutase (SOD) expression and decrease 8-iso-PGF2\u03b1 level). Furthermore, the GC-MS-based cerebrospinal fluid (CSF) metabolomics analysis indicated that Cop influenced the level of glycine, 2,3,4-trihydroxybutyric acid, oleic acid, glycerol, and ribose during IR injury. Cop exhibited a good neuroprotective effect against cerebral IR injury and metabolic alterations, which might be mediated through its antioxidant and anti-inflammatory properties.<\/jats:p>","DOI":"10.3390\/molecules28176350","type":"journal-article","created":{"date-parts":[[2023,8,30]],"date-time":"2023-08-30T10:30:52Z","timestamp":1693391452000},"page":"6350","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Gas Chromatography\u2013Mass Spectrometry-Based Cerebrospinal Fluid Metabolomics to Reveal the Protection of Coptisine against Transient Focal Cerebral Ischemia\u2013Reperfusion Injury via Anti-Inflammation and Antioxidant"],"prefix":"10.3390","volume":"28","author":[{"given":"Junjie","family":"Zhang","sequence":"first","affiliation":[{"name":"Key Laboratory of Chinese Medicinal Resource from Lingnan, Research Center of Chinese Herbal Resource Science and Engineering, School of Traditional Chinese Meteria Medica, Guangzhou University of Chinese Medicine, Ministry of Education, Guangzhou 510006, China"},{"name":"Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang 524023, China"},{"name":"School of Pharmacy, Guangdong Medical University, Zhanjiang 524023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ao","family":"Qi","sequence":"additional","affiliation":[{"name":"Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang 524023, China"},{"name":"School of Pharmacy, Guangdong Medical University, Zhanjiang 524023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lulu","family":"Liu","sequence":"additional","affiliation":[{"name":"Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang 524023, China"},{"name":"School of Pharmacy, Guangdong Medical University, Zhanjiang 524023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun","family":"Cai","sequence":"additional","affiliation":[{"name":"Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang 524023, China"},{"name":"School of Pharmacy, Guangdong Medical University, Zhanjiang 524023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Xu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Chinese Medicinal Resource from Lingnan, Research Center of Chinese Herbal Resource Science and Engineering, School of Traditional Chinese Meteria Medica, Guangzhou University of Chinese Medicine, Ministry of Education, Guangzhou 510006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e93","DOI":"10.1161\/CIR.0000000000001123","article-title":"Heart Disease and Stroke Statistics-2023 Update: A Report From the American Heart Association","volume":"147","author":"Tsao","year":"2023","journal-title":"Circulation"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1240","DOI":"10.1161\/STROKEAHA.119.027921","article-title":"Trends of r-tPA (Recombinant Tissue-Type Plasminogen Activator) Treatment and Treatment-Influencing Factors in Acute Ischemic Stroke","volume":"51","author":"Marko","year":"2020","journal-title":"Stroke"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1005301","DOI":"10.3389\/fphar.2022.1005301","article-title":"Serum and brain metabolomic study reveals the protective effects of Bai-Mi-Decoction on rats with ischemic stroke","volume":"13","author":"Yang","year":"2022","journal-title":"Front. 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