{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T13:10:20Z","timestamp":1792069820590,"version":"4.4.0"},"reference-count":56,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T00:00:00Z","timestamp":1693526400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Gen Foundation","award":["FOE-2021 DBA.AD005.225"],"award-info":[{"award-number":["FOE-2021 DBA.AD005.225"]}]},{"name":"CNR project","award":["FOE-2021 DBA.AD005.225"],"award-info":[{"award-number":["FOE-2021 DBA.AD005.225"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>The inhibition of carbohydrate digestion by plant bioactive compounds is a potential dietary strategy to counteract type 2 diabetes. Indeed, inhibition of \u03b1-amylase, a key enzyme that carries out the bulk of starch digestion, has been demonstrated for a range of bioactive compounds including anthocyanins; however, sample pigmentation often interferes with measurements, affecting colorimetric assay outcomes. Therefore, the present study compared the performance of a direct chromogenic assay, using 2-chloro-4 nitrophenyl \u03b1-D-maltotrioside (CNPG3) as a substrate, with the commonly used 3,5-dinitrosalicylic acid (DNS) assay. The direct chromogenic assay demonstrated a 5\u201310-fold higher sensitivity to determine \u03b1-amylase inhibition in various samples, including acarbose as a reference, pure anthocyanins, and anthocyanin-rich samples. The IC50 values of acarbose presented as 37.6 \u03bcg\/mL and 3.72 \u03bcg\/mL for the DNS assay and the direct chromogenic assay, respectively, whereas purified anthocyanins from blackcurrant showed IC50 values of 227.4 \u00b5g\/mL and 35.0 \u00b5g\/mL. The direct chromogenic assay is easy to perform, fast, reproducible, and suitable for high-throughput screening of pigmented \u03b1-amylase inhibitors.<\/jats:p>","DOI":"10.3390\/molecules28176399","type":"journal-article","created":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T09:12:22Z","timestamp":1693559542000},"page":"6399","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Chromogenic Assay Is More Efficient in Identifying \u03b1-Amylase Inhibitory Properties of Anthocyanin-Rich Samples When Compared to the 3,5-Dinitrosalicylic Acid (DNS) Assay"],"prefix":"10.3390","volume":"28","author":[{"given":"Sadia","family":"Zulfiqar","sequence":"first","affiliation":[{"name":"School of Food Science and Nutrition, Faculty of Environment, University of Leeds, Leeds LS2 9JT, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9417-2492","authenticated-orcid":false,"given":"Federica","family":"Blando","sequence":"additional","affiliation":[{"name":"Institute of Sciences of Food Production (ISPA), National Research Council (CNR), Via Prov.le Lecce-Monteroni, 73100 Lecce, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2564-8068","authenticated-orcid":false,"given":"Caroline","family":"Orfila","sequence":"additional","affiliation":[{"name":"School of Food Science and Nutrition, Faculty of Environment, University of Leeds, Leeds LS2 9JT, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8162-703X","authenticated-orcid":false,"given":"Lisa J.","family":"Marshall","sequence":"additional","affiliation":[{"name":"School of Food Science and Nutrition, Faculty of Environment, University of Leeds, Leeds LS2 9JT, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6705-5709","authenticated-orcid":false,"given":"Christine","family":"Boesch","sequence":"additional","affiliation":[{"name":"School of Food Science and Nutrition, Faculty of Environment, University of Leeds, Leeds LS2 9JT, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,9,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1155\/S1110724304407013","article-title":"Anthocyanins\u2014More than nature\u2019s colours","volume":"2004","author":"Konczak","year":"2004","journal-title":"J. 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