{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T21:00:23Z","timestamp":1793566823742,"version":"4.5.0"},"reference-count":45,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2023,9,4]],"date-time":"2023-09-04T00:00:00Z","timestamp":1693785600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100017575","name":"Hue University","doi-asserted-by":"publisher","award":["DHH2021-04-153"],"award-info":[{"award-number":["DHH2021-04-153"]}],"id":[{"id":"10.13039\/501100017575","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Curcumin (CUR) is a natural compound extracted from turmeric (Curcuma longa L.) used to cure acne, wound healing, etc. Its disadvantages, such as poor solubility and permeability, limit its efficacy. Nanoemulsion (NE)-based drug delivery systems have gained popularity due to their advantages. This study aimed to optimize a CUR-NE-based gel and evaluate its physicochemical and biological properties. A NE was prepared using the catastrophic phase inversion method and optimized using the Design Expert 12.0 software. The CUR-NE gel was characterized in terms of visual appearance, pH, drug release, antibacterial and wound healing effects. The optimal formulation contained CUR, Capryol 90 (oil), Labrasol:Cremophor RH40 (1:1) (surfactants), propylene glycol (co-surfactant), and water. The NE had a droplet size of 22.87 nm and a polydispersity index of 0.348. The obtained CUR-NE gel had a soft, smooth texture and a pH of 5.34 \u00b1 0.05. The in vitro release of CUR from the NE-based gel was higher than that from a commercial gel with nanosized CUR (21.68 \u00b1 1.25 \u00b5g\/cm2, 13.62 \u00b1 1.63 \u00b5g\/cm2 after 10 h, respectively). The CUR-NE gel accelerated in vitro antibacterial and in vivo wound healing activities as compared to other CUR-loaded gels. The CUR-NE gel has potential for transdermal applications.<\/jats:p>","DOI":"10.3390\/molecules28176433","type":"journal-article","created":{"date-parts":[[2023,9,5]],"date-time":"2023-09-05T10:26:43Z","timestamp":1693909603000},"page":"6433","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Development and Characterization of a Hydrogel Containing Curcumin-Loaded Nanoemulsion for Enhanced In Vitro Antibacteria and In Vivo Wound Healing"],"prefix":"10.3390","volume":"28","author":[{"given":"Thi Thanh Ngoc","family":"Le","sequence":"first","affiliation":[{"name":"Faculty of Pharmacy, University of Medicine and Pharmacy, Hue University, 6 Ngo Quyen, Hue 530000, Thua Thien Hue, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thi Kieu Nhi","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, University of Medicine and Pharmacy, Hue University, 6 Ngo Quyen, Hue 530000, Thua Thien Hue, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Van Minh","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Faculty of Odonto-Stomatology, University of Medicine and Pharmacy, Hue University, 6 Ngo Quyen, Hue 530000, Thua Thien Hue, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0786-3178","authenticated-orcid":false,"given":"Thi Cam Minh","family":"Dao","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, University of Medicine and Pharmacy, Hue University, 6 Ngo Quyen, Hue 530000, Thua Thien Hue, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hoai Bao Chau","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, University of Medicine and Pharmacy, Hue University, 6 Ngo Quyen, Hue 530000, Thua Thien Hue, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cong Thuan","family":"Dang","sequence":"additional","affiliation":[{"name":"Department of Histology, Embryology, Pathology, and Forensic, University of Medicine and Pharmacy, Hue University, 6 Ngo Quyen, Hue 530000, Thua Thien Hue, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5093-8094","authenticated-orcid":false,"given":"Thi Bao Chi","family":"Le","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Medicine and Pharmacy, Hue University, 6 Ngo Quyen, Hue 530000, Thua Thien Hue, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thi Khanh Linh","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Medicine and Pharmacy, Hue University, 6 Ngo Quyen, Hue 530000, Thua Thien Hue, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phuong Thao Tien","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Department of Histology, Embryology, Pathology, and Forensic, University of Medicine and Pharmacy, Hue University, 6 Ngo Quyen, Hue 530000, Thua Thien Hue, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Le Hoang Nam","family":"Dang","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Surgical Training, University of Medicine and Pharmacy, Hue University, 6 Ngo Quyen, Hue 530000, Thua Thien Hue, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Van Minh","family":"Doan","sequence":"additional","affiliation":[{"name":"Faculty of Traditional Medicine, University of Medicine and Pharmacy, Hue University, 6 Ngo Quyen, Hue 530000, Thua Thien Hue, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9522-6288","authenticated-orcid":false,"given":"Hoang Nhan","family":"Ho","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, University of Medicine and Pharmacy, Hue University, 6 Ngo Quyen, Hue 530000, Thua Thien Hue, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,9,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1926","DOI":"10.1002\/ptr.6658","article-title":"Anti-microbial activity of curcumin nanoformulations: New trends and future perspectives","volume":"34","author":"Sharifi","year":"2020","journal-title":"Phytother. Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2","DOI":"10.4143\/crt.2014.46.1.2","article-title":"Recent developments in delivery, bioavailability, absorption and metabolism of curcumin: The golden pigment from golden spice","volume":"46","author":"Prasad","year":"2014","journal-title":"Cancer Res. Treat."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"46494","DOI":"10.1021\/acsomega.2c05293","article-title":"Comparison of Antibacterial Activity of Nanocurcumin with Bulk Curcumin","volume":"7","author":"Hettiarachchi","year":"2022","journal-title":"ACS Omega"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"98","DOI":"10.3109\/21691401.2015.1129628","article-title":"Enhanced anti-cancer and antimicrobial activities of curcumin nanoparticles","volume":"45","author":"Adahoun","year":"2017","journal-title":"Artif. Cells Nanomed. Biotechnol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2487","DOI":"10.1002\/ptr.6976","article-title":"Curcumin nanoformulations for antimicrobial and wound healing purposes","volume":"35","author":"Salehi","year":"2021","journal-title":"Phytother. Res."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1039\/C9FO00901A","article-title":"Enhanced curcumin solubility and antibacterial activity by encapsulation in PLGA oily core nanocapsules","volume":"11","author":"Gao","year":"2020","journal-title":"Food Funct."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1676","DOI":"10.1080\/10717544.2020.1853280","article-title":"Curcumin nanoparticles incorporated in PVA\/collagen composite films promote wound healing","volume":"27","author":"Leng","year":"2020","journal-title":"Drug Deliv."},{"key":"ref_8","first-page":"13","article-title":"Improving Antibacterial Efficiency of Curcumin in Magnetic Polymeric Nanocomposites","volume":"18","author":"Behjou","year":"2022","journal-title":"J. Pharm. Innov."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1007\/s13346-017-0405-4","article-title":"Evaluation of propylene glycol nanoliposomes containing curcumin on burn wound model in rat: Biocompatibility, wound healing, and anti-bacterial effects","volume":"7","author":"Kianvash","year":"2017","journal-title":"Drug Deliv. Transl. Res."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"17","DOI":"10.15171\/apb.2016.04","article-title":"Sustained release Curcumin loaded Solid Lipid Nanoparticles","volume":"6","author":"Jourghanian","year":"2016","journal-title":"Adv. Pharm. Bull."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1186\/s12951-021-00851-2","article-title":"Synthesis and self-assembly of curcumin-modified amphiphilic polymeric micelles with antibacterial activity","volume":"19","author":"Barros","year":"2021","journal-title":"J. Nanobiotechnol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1080\/17425247.2022.2045944","article-title":"Nanoemulsion-based dosage forms for the transdermal drug delivery applications: A review of recent advances","volume":"19","author":"Roy","year":"2022","journal-title":"Expert Opin. Drug Deliv."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2826","DOI":"10.1039\/C5SM02958A","article-title":"Nanoemulsions: Formation, properties and applications","volume":"12","author":"Gupta","year":"2016","journal-title":"Soft Matter"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"65","DOI":"10.4103\/2230-973X.133053","article-title":"Enhanced transdermal permeability of piroxicam through novel nanoemulgel formulation","volume":"4","author":"Dhawan","year":"2014","journal-title":"Int. J. Pharm. Investig."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1186\/s12951-017-0323-0","article-title":"Development of triptolide-nanoemulsion gels for percutaneous administration: Physicochemical, transport, pharmacokinetic and pharmacodynamic characteristics","volume":"15","author":"Yang","year":"2017","journal-title":"J. Nanobiotechnol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"20192","DOI":"10.1039\/C9RA03102B","article-title":"Preparation of a novel curcumin nanoemulsion by ultrasonication and its comparative effects in wound healing and the treatment of inflammation","volume":"9","author":"Ahmad","year":"2019","journal-title":"RSC Adv."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1080\/00405000.2018.1477319","article-title":"Novel approach for development of eco-friendly antimicrobial textile material for health care application","volume":"110","author":"Kole","year":"2018","journal-title":"J. Text. Inst."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"7669255","DOI":"10.1155\/2022\/7669255","article-title":"Novel Curcumin-Encapsulated alpha-Tocopherol Nanoemulsion System and Its Potential Application for Wound Healing in Diabetic Animals","volume":"2022","author":"Ali","year":"2022","journal-title":"BioMed Res. Int."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"101847","DOI":"10.1016\/j.jddst.2020.101847","article-title":"Nanoemulsion loaded polymeric hydrogel for topical delivery of curcumin in psoriasis","volume":"59","author":"Algahtani","year":"2020","journal-title":"J. Drug Deliv. Sci. Technol."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Algahtani, M.S., Ahmad, M.Z., Nourein, I.H., Albarqi, H.A., Alyami, H.S., Alyami, M.H., Alqahtani, A.A., Alasiri, A., Algahtani, T.S., and Mohammed, A.A. (2021). Preparation and Characterization of Curcumin Nanoemulgel Utilizing Ultrasonication Technique for Wound Healing: In Vitro, Ex Vivo, and In Vivo Evaluation. Gels, 7.","DOI":"10.3390\/gels7040213"},{"key":"ref_21","unstructured":"Nguyen, T.K.N. (2022). Optimization of Nanoemulsion Containing Curcumin (in Vietnamese). [Bachelor\u2019s Thesis, University of Medicine and Pharmacy, Hue University]."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"560","DOI":"10.3109\/03639045.2014.884127","article-title":"Curcumin nanoemulsion for transdermal application: Formulation and evaluation","volume":"41","author":"Rachmawati","year":"2015","journal-title":"Drug Dev. Ind. Pharm."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/j.jconrel.2017.11.049","article-title":"Nanoemulsion as pharmaceutical carrier for dermal and transdermal drug delivery: Formulation development, stability issues, basic considerations and applications","volume":"270","author":"Rai","year":"2018","journal-title":"J. Control. Release"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/j.ejps.2014.10.014","article-title":"D-optimal experimental approach for designing topical microemulsion of itraconazole: Characterization and evaluation of antifungal efficacy against a standardized Tinea pedis infection model in Wistar rats","volume":"67","author":"Kumar","year":"2015","journal-title":"Eur. J. Pharm. Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1016\/j.ijpharm.2013.06.009","article-title":"Microemulsion and poloxamer microemulsion-based gel for sustained transdermal delivery of diclofenac epolamine using in-skin drug depot: In vitro\/in vivo evaluation","volume":"453","author":"Fouad","year":"2013","journal-title":"Int. J. Pharm."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Sarheed, O., Dibi, M., and Ramesh, K. (2020). Studies on the Effect of Oil and Surfactant on the Formation of Alginate-Based O\/W Lidocaine Nanocarriers Using Nanoemulsion Template. Pharmaceutics, 12.","DOI":"10.3390\/pharmaceutics12121223"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/j.carbpol.2018.08.079","article-title":"Pickering emulsions stabilized nanocellulosic-based nanoparticles for coumarin and curcumin nanoencapsulations: In vitro release, anticancer and antimicrobial activities","volume":"201","author":"Yildiz","year":"2018","journal-title":"Carbohydr. Polym."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.tifs.2017.12.004","article-title":"Impact of curcumin nanoformulation on its antimicrobial activity","volume":"72","author":"Silva","year":"2018","journal-title":"Trends Food Sci. Technol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/j.nano.2014.09.004","article-title":"Curcumin-encapsulated nanoparticles as innovative antimicrobial and wound healing agent","volume":"11","author":"Krausz","year":"2015","journal-title":"Nanomedicine"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1111\/j.1742-481X.2012.01114.x","article-title":"Effects of potassium apigenin and verbena extract on the wound healing process of SKH-1 mouse skin","volume":"11","author":"Canas","year":"2014","journal-title":"Int. Wound J."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Nguyen, V.L., Truong, C.T., Nguyen, B.C.Q., Vo, T.V., Dao, T.T., Nguyen, V.D., Trinh, D.T., Huynh, H.K., and Bui, C.B. (2017). Anti-inflammatory and wound healing activities of calophyllolide isolated from Calophyllum inophyllum Linn. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0185674"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1003","DOI":"10.1007\/s00217-014-2297-6","article-title":"The anti-inflammatory and wound healing properties of honey","volume":"239","author":"Hadagali","year":"2014","journal-title":"Eur. Food Res. Technol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1016\/j.ijpharm.2016.04.052","article-title":"Emu oil based nano-emulgel for topical delivery of curcumin","volume":"506","author":"Jeengar","year":"2016","journal-title":"Int. J. Pharm."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Sideek, S.A., El-Nassan, H.B., Fares, A.R., ElMeshad, A.N., and Elkasabgy, N.A. (2022). Different Curcumin-Loaded Delivery Systems for Wound Healing Applications: A Comprehensive Review. Pharmaceutics, 15.","DOI":"10.3390\/pharmaceutics15010038"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Omidian, H., Wilson, R.L., and Chowdhury, S.D. (2023). Enhancing Therapeutic Efficacy of Curcumin: Advances in Delivery Systems and Clinical Applications. Gels, 9.","DOI":"10.3390\/gels9080596"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"124960","DOI":"10.1016\/j.ijbiomac.2023.124960","article-title":"Rapidly degrading and mussel-inspired multifunctional carboxymethyl chitosan\/montmorillonite hydrogel for wound hemostasis","volume":"242","author":"Ouyang","year":"2023","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Dokovic, J.B., Savic, S.M., Mitrovic, J.R., Nikolic, I., Markovic, B.D., Randjelovic, D.V., Antic-Stankovic, J., Bozic, D., Cekic, N.D., and Stevanovic, V. (2021). Curcumin Loaded PEGylated Nanoemulsions Designed for Maintained Antioxidant Effects and Improved Bioavailability: A Pilot Study on Rats. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22157991"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.jsps.2019.11.008","article-title":"Evaluation of wound healing effect of chitosan-based gel formulation containing vitexin","volume":"28","author":"Bektas","year":"2020","journal-title":"Saudi Pharm. J."},{"key":"ref_39","first-page":"112677","article-title":"Development of PVA-based microsphere as a potential embolization agent","volume":"135","author":"Yang","year":"2022","journal-title":"Mater. Sci. Eng. C Mater. Biol. Appl."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Scomoroscenco, C., Teodorescu, M., Raducan, A., Stan, M., Voicu, S.N., Trica, B., Ninciuleanu, C.M., Nistor, C.L., Mihaescu, C.I., and Petcu, C. (2021). Novel Gel Microemulsion as Topical Drug Delivery System for Curcumin in Dermatocosmetics. Pharmaceutics, 13.","DOI":"10.3390\/pharmaceutics13040505"},{"key":"ref_41","unstructured":"U.S. Food and Drug Administration (2023, August 27). Inactive Ingredient Search for Approved Drug Products, Available online: https:\/\/www.accessdata.fda.gov\/scripts\/cder\/iig\/index.cfm."},{"key":"ref_42","unstructured":"U.S. Food and Drug Administration (2023, August 27). SCOGS (Select Committee on GRAS Substances), Available online: https:\/\/www.cfsanappsexternal.fda.gov\/scripts\/fdcc\/?set=SCOGS."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1016\/j.ijbiomac.2017.03.066","article-title":"Development of Curcumin loaded chitosan polymer based nanoemulsion gel: In vitro, ex vivo evaluation and in vivo wound healing studies","volume":"101","author":"Thomas","year":"2017","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1010","DOI":"10.1016\/j.ijbiomac.2021.11.161","article-title":"Formulation and characterization of hydroxyethyl cellulose-based gel containing metronidazole-loaded solid lipid nanoparticles for buccal mucosal drug delivery","volume":"194","author":"Ho","year":"2022","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_45","unstructured":"Clinical and Laboratory Standards Institute (2020). CLSI Supplement M100, CLSI."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/28\/17\/6433\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:46:27Z","timestamp":1760129187000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/28\/17\/6433"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,9,4]]},"references-count":45,"journal-issue":{"issue":"17","published-online":{"date-parts":[[2023,9]]}},"alternative-id":["molecules28176433"],"URL":"https:\/\/doi.org\/10.3390\/molecules28176433","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,9,4]]}}}