{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T19:38:50Z","timestamp":1793216330930,"version":"4.5.0"},"reference-count":31,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2023,9,7]],"date-time":"2023-09-07T00:00:00Z","timestamp":1694044800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China","award":["31972750"],"award-info":[{"award-number":["31972750"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Endoplasmic reticulum (ER) stress is a crucial factor in the pathogenesis of intestinal diseases. Soybean antigenic proteins (\u03b2-conglycinin and soy glycinin) induce hypersensitivity reactions and intestinal barrier damage. However, whether this damage is associated with ER stress, autophagy, and the gut microbiome is largely unclear. Therefore, in this study, we aimed to investigate the effect of dietary supplementation with soy glycinin (11S glycinin) and \u03b2-conglycinin (7S glycinin) on intestinal ER stress, autophagy, and flora in weaned piglets. Thirty healthy 21-day-old weaned \u201cDuroc \u00d7 Long White \u00d7 Yorkshire\u201d piglets were randomly divided into three groups and fed a basic, 7S-supplemented, or 11S-supplemented diet for one week. The results indicated that 7S\/11S glycinin disrupted growth performance, damaged intestinal barrier integrity, and impaired goblet cell function in piglets (p &lt; 0.05). Moreover, 7S\/11S glycinin induced ER stress and blocked autophagic flux in the jejunum (p &lt; 0.05) and increased the relative abundance of pathogenic flora (p &lt; 0.01) and decreased that of beneficial flora (p &lt; 0.05). In conclusion, 7S\/11S glycinin induces intestinal ER stress, autophagic flux blockage, microbiota imbalance, and intestinal barrier damage in piglets.<\/jats:p>","DOI":"10.3390\/molecules28186500","type":"journal-article","created":{"date-parts":[[2023,9,7]],"date-time":"2023-09-07T10:09:50Z","timestamp":1694081390000},"page":"6500","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Soybean Antigen Protein-Induced Intestinal Barrier Damage by Trigging Endoplasmic Reticulum Stress and Disordering Gut Microbiota in Weaned Piglets"],"prefix":"10.3390","volume":"28","author":[{"given":"Lei","family":"Wang","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology, Anhui Agricultural University, Hefei 230061, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujia","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Anhui Agricultural University, Hefei 230061, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daoliang","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Anhui Agricultural University, Hefei 230061, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongyan","family":"Ding","sequence":"additional","affiliation":[{"name":"Anhui Provincial Key Laboratory of Livestock and Poultry Product Safety Engineering, Institute of Animal Husbandry and Veterinary Medicine, Medicine Academy of Agricultural Sciences, Hefei 230061, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shibin","family":"Feng","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Anhui Agricultural University, Hefei 230061, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8668-0171","authenticated-orcid":false,"given":"Chang","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Anhui Agricultural University, Hefei 230061, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinjie","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Anhui Agricultural University, Hefei 230061, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5096-3970","authenticated-orcid":false,"given":"Xichun","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Anhui Agricultural University, Hefei 230061, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,9,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2825","DOI":"10.3389\/fimmu.2019.02825","article-title":"ER Stress and the UPR in Shaping Intestinal Tissue Homeostasis and Immunity","volume":"10","author":"Coleman","year":"2019","journal-title":"Front. 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