{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T14:28:21Z","timestamp":1787063301868,"version":"build-2736575974"},"reference-count":62,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2023,9,8]],"date-time":"2023-09-08T00:00:00Z","timestamp":1694131200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Secreted phospholipases A2 are snake-venom proteins with many biological activities, notably anti-tumor activity. Phospholipases from the same snake type but different geographical locations have shown similar biochemical and biological activities with minor differences in protein sequences. Thus, the discovery of a new phospholipase A2 with unique characteristics identified in a previously studied venom could suggest the origins of these differences. Here, a new Group II secreted phospholipase A2 (Cc-PLA2-II) from the snake venom of Saudi Cerastes cerastes gasperetti was isolated and characterized. The purified enzyme had a molecular weight of 13.945 kDa and showed high specific activity on emulsified phosphatidylcholine of 1560 U\/mg at pH 9.5 and 50 \u00b0C with strict calcium dependence. Interestingly, stability in extreme pH and high temperatures was observed after enzyme incubation at several pH levels and temperatures. Moreover, a significant dose-dependent cytotoxic anti-tumor effect against six human cancer cell lines was observed with concentrations of Cc-PLA2 ranging from 2.5 to 8 \u00b5M. No cytotoxic effect on normal human umbilical-vein endothelial cells was noted. These results suggest that Cc-PLA2-II potentially has angiogenic activity of besides cytotoxicity as part of its anti-tumor mechanism. This study justifies the inclusion of this enzyme in many applications for anticancer drug development.<\/jats:p>","DOI":"10.3390\/molecules28186517","type":"journal-article","created":{"date-parts":[[2023,9,8]],"date-time":"2023-09-08T08:01:30Z","timestamp":1694160090000},"page":"6517","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Functional Characterization and Anti-Tumor Effect of a Novel Group II Secreted Phospholipase A2 from Snake Venom of Saudi Cerastes cerates gasperetti"],"prefix":"10.3390","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9430-7980","authenticated-orcid":false,"given":"Mona","family":"Alonazi","sequence":"first","affiliation":[{"name":"Biochemistry Department, Science College, King Saud University, P.O. Box 22452, Riyadh 11495, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Najeh","family":"Krayem","sequence":"additional","affiliation":[{"name":"Laboratoire de Biochimie et de G\u00e9nie Enzymatique des Lipases, ENIS, Universit\u00e9 de Sfax, Route de Soukra 3038, Sfax BP 1173, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mona G.","family":"Alharbi","sequence":"additional","affiliation":[{"name":"Biochemistry Department, Science College, King Saud University, P.O. Box 22452, Riyadh 11495, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3376-7008","authenticated-orcid":false,"given":"Arwa Ishaq A.","family":"Khayyat","sequence":"additional","affiliation":[{"name":"Biochemistry Department, Science College, King Saud University, P.O. Box 22452, Riyadh 11495, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2079-7577","authenticated-orcid":false,"given":"Humidah","family":"Alanazi","sequence":"additional","affiliation":[{"name":"Biochemistry Department, Science College, King Saud University, P.O. Box 22452, Riyadh 11495, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Habib","family":"Horchani","sequence":"additional","affiliation":[{"name":"Science Department, College of Rivi\u00e8re-Du-Loup, Rivi\u00e8re-Du-Loup, QC G5R 1R1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6372-7483","authenticated-orcid":false,"given":"Abir","family":"Ben Bacha","sequence":"additional","affiliation":[{"name":"Biochemistry Department, Science College, King Saud University, P.O. Box 22452, Riyadh 11495, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,9,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.sjbs.2015.10.010","article-title":"Ecological aspects of the horned viper, Cerastes cerastes gasperettii in the central region of Saudi Arabia","volume":"23","author":"Paray","year":"2016","journal-title":"Saudi J. Biol. Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"925","DOI":"10.1016\/j.sjbs.2017.02.007","article-title":"Survey of the reptilian fauna of the Kingdom of Saudi Arabia. VI. The snake fauna of Turaif region","volume":"24","author":"Paray","year":"2017","journal-title":"Saudi J. Biol. 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