{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T11:27:30Z","timestamp":1791804450332,"version":"4.3.4"},"reference-count":41,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2023,11,24]],"date-time":"2023-11-24T00:00:00Z","timestamp":1700784000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Jilin Province Science and Technology Development Key Program","award":["20180201009NY"],"award-info":[{"award-number":["20180201009NY"]}]},{"name":"Jilin Province Science and Technology Development Key Program","award":["222604YY0102102808"],"award-info":[{"award-number":["222604YY0102102808"]}]},{"name":"Jilin Province Science and Technology Development Key Program","award":["2022220101000180"],"award-info":[{"award-number":["2022220101000180"]}]},{"name":"Jilin Province Science and Technology Development Key Program","award":["3G016W112418"],"award-info":[{"award-number":["3G016W112418"]}]},{"name":"the cross-regional cooperation program of research institutes","award":["20180201009NY"],"award-info":[{"award-number":["20180201009NY"]}]},{"name":"the cross-regional cooperation program of research institutes","award":["222604YY0102102808"],"award-info":[{"award-number":["222604YY0102102808"]}]},{"name":"the cross-regional cooperation program of research institutes","award":["2022220101000180"],"award-info":[{"award-number":["2022220101000180"]}]},{"name":"the cross-regional cooperation program of research institutes","award":["3G016W112418"],"award-info":[{"award-number":["3G016W112418"]}]},{"name":"the development plan project during \u201cThe 13th Five Year Plan\u201d for Nation Science and Technology in rural areas","award":["20180201009NY"],"award-info":[{"award-number":["20180201009NY"]}]},{"name":"the development plan project during \u201cThe 13th Five Year Plan\u201d for Nation Science and Technology in rural areas","award":["222604YY0102102808"],"award-info":[{"award-number":["222604YY0102102808"]}]},{"name":"the development plan project during \u201cThe 13th Five Year Plan\u201d for Nation Science and Technology in rural areas","award":["2022220101000180"],"award-info":[{"award-number":["2022220101000180"]}]},{"name":"the development plan project during \u201cThe 13th Five Year Plan\u201d for Nation Science and Technology in rural areas","award":["3G016W112418"],"award-info":[{"award-number":["3G016W112418"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Taxanes are the best-known compounds in Taxus cuspidata owing to their strong anticancer effects. However, the traditional taxanes extraction method is the solid\u2013liquid extraction method, which is limited by a large energy consumption and low yield. Therefore, it is urgent to find an efficient method for taxanes extraction. The ultrasonic microwave synergistic extraction (UME) method integrates the cavitation effect of ultrasound and the intensifying heat transfer (ionic conduction and dipole rotation of molecules) effect of microwave to accelerate the release of intracellular compounds and is used in active ingredient extractions. This study aimed to evaluate the performance of UME in extracting taxanes from T. cuspidata needles (dichloromethane-ethanol as extractant). A single-factor experiment, Plackett\u2013Burman design, and the response surface method showed that the optimal UME parameters for taxanes extraction were an ultrasonic power of 300 W, a microwave power of 215 W, and 130 sieve meshes. Under these conditions, the taxanes yield was 570.32 \u03bcg\/g, which increased by 13.41% and 41.63% compared with the ultrasound (US) and microwave (MW) treatments, respectively. The reasons for the differences in the taxanes yield were revealed by comparing the physicochemical properties of T. cuspidata residues after the UME, US, and MW treatments. The cell structures were significantly damaged after the UME treatment, and numerous tiny holes were observed on the surface. The absorption peaks of cellulose, hemicellulose, and lignin increased significantly in intensity, and the lowest peak temperature (307.40 \u00b0C), with a melting enthalpy of \u22125.19 J\/g, was found after the UME treatment compared with the US and MW treatments. These results demonstrate that UME is an effective method (570.32 \u03bcg\/g) to extract taxanes from T. cuspidata needles by destroying cellular structures.<\/jats:p>","DOI":"10.3390\/molecules28237746","type":"journal-article","created":{"date-parts":[[2023,11,24]],"date-time":"2023-11-24T06:51:28Z","timestamp":1700808688000},"page":"7746","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Parameter Optimization of Ultrasonic\u2013Microwave Synergistic Extraction of Taxanes from Taxus cuspidata Needles"],"prefix":"10.3390","volume":"28","author":[{"given":"Zirui","family":"Zhao","sequence":"first","affiliation":[{"name":"College of Biology and Agricultural Engineering, Jilin University, Changchun 130022, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yajing","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Biology and Agricultural Engineering, Jilin University, Changchun 130022, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenlong","family":"Li","sequence":"additional","affiliation":[{"name":"College of Biology and Agricultural Engineering, Jilin University, Changchun 130022, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanhu","family":"Tang","sequence":"additional","affiliation":[{"name":"College of Biology and Agricultural Engineering, Jilin University, Changchun 130022, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6629-975X","authenticated-orcid":false,"given":"Shujie","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Biology and Agricultural Engineering, Jilin University, Changchun 130022, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,11,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1515\/biol-2021-0032","article-title":"Investigation of bioactivities of Taxus chinensis, Taxus cuspidata, and Taxus x media by gas chromatography-mass spectrometry","volume":"16","author":"Zhang","year":"2021","journal-title":"Open Life Sci."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Sabzehzari, M., Zeinali, M., and Naghavi, M.R. 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