{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T17:10:15Z","timestamp":1785345015266,"version":"3.55.0"},"reference-count":38,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2023,11,28]],"date-time":"2023-11-28T00:00:00Z","timestamp":1701129600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004329","name":"Slovenian Research Agency","doi-asserted-by":"publisher","award":["P1-0189"],"award-info":[{"award-number":["P1-0189"]}],"id":[{"id":"10.13039\/501100004329","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Ad Futura Scientific and Educational Foundation of the Republic of Slovenia","award":["P1-0189"],"award-info":[{"award-number":["P1-0189"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>The objective of the present report was to develop and validate a simple, selective, and reproducible high-performance liquid chromatography method with UV detection suitable for routine therapeutic drug monitoring of the most commonly used antiepileptic drugs and some of their metabolites. Simple precipitation of plasma proteins with acetonitrile was used for sample preparation. 10,11-dihydrocarbamazepine was used as an internal standard. Chromatographic separation of the analytes was achieved by gradient elution on a Phenyl\u2013Hexyl column at 40 \u00b0C, using methanol and potassium phosphate buffer (25 mM; pH 5.1) as a mobile phase. The method was validated according to the FDA guidelines for bioanalytical method validation. It showed to be selective, accurate, precise, and linear over the concentration ranges of 1\u201350 mg\/L for phenobarbital, phenytoin, levetiracetam, rufinamide, zonisamide, and lacosamide; 0.5\u201350 mg\/L for lamotrigine, primidone, carbamazepine and 10-monohydroxycarbazepine; 0.2\u201310 mg\/L for carbamazepine metabolites: 10,11-trans-dihydroxy-10,11-dihydrocarbamazepine and carbamazepine-10,11-epoxide; 0.1\u201310 mg\/L for oxcarbazepine; 2\u2013100 mg\/L for felbamate and 3\u2013150 mg\/L for ethosuximide. The suitability of the validated method for routine therapeutic drug monitoring was confirmed by quantification of the analytes in plasma samples from patients with epilepsy on combination antiepileptic therapy.<\/jats:p>","DOI":"10.3390\/molecules28237830","type":"journal-article","created":{"date-parts":[[2023,11,28]],"date-time":"2023-11-28T11:43:16Z","timestamp":1701171796000},"page":"7830","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Simple HPLC-UV Method for Therapeutic Drug Monitoring of 12 Antiepileptic Drugs and Their Main Metabolites in Human Plasma"],"prefix":"10.3390","volume":"28","author":[{"given":"Daniela","family":"Milosheska","sequence":"first","affiliation":[{"name":"University of Ljubljana, Faculty of Pharmacy, A\u0161ker\u010deva Cesta 7, 1000 Ljubljana, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9863-9117","authenticated-orcid":false,"given":"Robert","family":"Ro\u0161kar","sequence":"additional","affiliation":[{"name":"University of Ljubljana, Faculty of Pharmacy, A\u0161ker\u010deva Cesta 7, 1000 Ljubljana, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,11,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1080\/17425255.2020.1724956","article-title":"Therapeutic Drug Monitoring of Antiepileptic Drugs: Current Status and Future Prospects","volume":"16","author":"Johannessen","year":"2020","journal-title":"Expert Opin. 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