{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,18]],"date-time":"2026-04-18T04:34:39Z","timestamp":1776486879194,"version":"3.51.2"},"reference-count":47,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2023,12,4]],"date-time":"2023-12-04T00:00:00Z","timestamp":1701648000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Agricultural Research Service (ARS) Research Participation Program administered by the Oak Ridge Institute for Science and Education (ORISE) through an interagency agreement between the U.S. Department of Energy (DOE) and the U.S. Department of Agriculture (USDA)","award":["DE-SC0014664"],"award-info":[{"award-number":["DE-SC0014664"]}]},{"name":"Agricultural Research Service (ARS) Research Participation Program administered by the Oak Ridge Institute for Science and Education (ORISE) through an interagency agreement between the U.S. Department of Energy (DOE) and the U.S. Department of Agriculture (USDA)","award":["58-6060-6-015"],"award-info":[{"award-number":["58-6060-6-015"]}]},{"DOI":"10.13039\/100006229","name":"ORAU","doi-asserted-by":"publisher","award":["DE-SC0014664"],"award-info":[{"award-number":["DE-SC0014664"]}],"id":[{"id":"10.13039\/100006229","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006229","name":"ORAU","doi-asserted-by":"publisher","award":["58-6060-6-015"],"award-info":[{"award-number":["58-6060-6-015"]}],"id":[{"id":"10.13039\/100006229","id-type":"DOI","asserted-by":"publisher"}]},{"name":"USDA Cooperative Agreement","award":["DE-SC0014664"],"award-info":[{"award-number":["DE-SC0014664"]}]},{"name":"USDA Cooperative Agreement","award":["58-6060-6-015"],"award-info":[{"award-number":["58-6060-6-015"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>The identification of natural and environmentally friendly pesticides is a key area of interest for the agrochemical industry, with many potentially active compounds being sourced from numerous plant species. In this study, we report the bioassay-guided isolation and identification of phytotoxic and antifungal compounds from the ethyl acetate extract of Helietta parvifolia stems. We identified eight compounds, consisting of two coumarins and six alkaloids. Among these, a new alkaloid, 2-hydroxy-3,6,7-trimethoxyquinoline-4-carbaldehyde (6), was elucidated, along with seven known compounds. The phytotoxicity of purified compounds was evaluated, and chalepin (4) was active against Agrostis stolonifera at 1 mM with 50% inhibition of seed germination and it reduced Lemna pausicotata (duckweed) growth by 50% (IC50) at 168 \u03bcM. Additionally, we evaluated the antifungal activity against the fungal plant pathogen Colletotrichum fragariae using a thin-layer chromatography bioautography assay, which revealed that three isolated furoquinoline alkaloids (flindersiamine (3), kokusagenine (7), and maculine (8)) among the isolated compounds had the strongest inhibitory effects on the growth of C. fragariae at all tested concentrations. Our results indicate that these active natural compounds, i.e., (3), (4), (7), and (8), could be scaffolds for the production of more active pesticides with better physicochemical properties.<\/jats:p>","DOI":"10.3390\/molecules28237930","type":"journal-article","created":{"date-parts":[[2023,12,4]],"date-time":"2023-12-04T07:59:31Z","timestamp":1701676771000},"page":"7930","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Antifungal and Phytotoxic Activities of Isolated Compounds from Helietta parvifolia Stems"],"prefix":"10.3390","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5015-2446","authenticated-orcid":false,"given":"Victor Pena","family":"Ribeiro","sequence":"first","affiliation":[{"name":"Agricultural Research Service, Natural Products Utilization Research Unit, U.S. Department of Agriculture, University, MS 38677, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joanna","family":"Bajsa-Hirschel","sequence":"additional","affiliation":[{"name":"Agricultural Research Service, Natural Products Utilization Research Unit, U.S. Department of Agriculture, University, MS 38677, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Prabin","family":"Tamang","sequence":"additional","affiliation":[{"name":"Agricultural Research Service, Natural Products Utilization Research Unit, U.S. Department of Agriculture, University, MS 38677, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kumudini","family":"Meepagala","sequence":"additional","affiliation":[{"name":"Agricultural Research Service, Natural Products Utilization Research Unit, U.S. Department of Agriculture, University, MS 38677, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen O.","family":"Duke","sequence":"additional","affiliation":[{"name":"National Center for Natural Products Research, University, MS 38677, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,12,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Baky, N.A., and Amara, A.A.A.F. 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