{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T17:48:04Z","timestamp":1774633684068,"version":"3.50.1"},"reference-count":32,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2023,12,12]],"date-time":"2023-12-12T00:00:00Z","timestamp":1702339200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004761","name":"Natural Science Foundation of Hainan Province","doi-asserted-by":"publisher","award":["822MS077"],"award-info":[{"award-number":["822MS077"]}],"id":[{"id":"10.13039\/501100004761","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004761","name":"Natural Science Foundation of Hainan Province","doi-asserted-by":"publisher","award":["2019RC216"],"award-info":[{"award-number":["2019RC216"]}],"id":[{"id":"10.13039\/501100004761","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004761","name":"Natural Science Foundation of Hainan Province","doi-asserted-by":"publisher","award":["Hnky2023-22"],"award-info":[{"award-number":["Hnky2023-22"]}],"id":[{"id":"10.13039\/501100004761","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004761","name":"Natural Science Foundation of Hainan Province","doi-asserted-by":"publisher","award":["XRC202029"],"award-info":[{"award-number":["XRC202029"]}],"id":[{"id":"10.13039\/501100004761","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Higher Education Institutions of Hainan Province","award":["822MS077"],"award-info":[{"award-number":["822MS077"]}]},{"name":"Higher Education Institutions of Hainan Province","award":["2019RC216"],"award-info":[{"award-number":["2019RC216"]}]},{"name":"Higher Education Institutions of Hainan Province","award":["Hnky2023-22"],"award-info":[{"award-number":["Hnky2023-22"]}]},{"name":"Higher Education Institutions of Hainan Province","award":["XRC202029"],"award-info":[{"award-number":["XRC202029"]}]},{"name":"Hainan Medical University","award":["822MS077"],"award-info":[{"award-number":["822MS077"]}]},{"name":"Hainan Medical University","award":["2019RC216"],"award-info":[{"award-number":["2019RC216"]}]},{"name":"Hainan Medical University","award":["Hnky2023-22"],"award-info":[{"award-number":["Hnky2023-22"]}]},{"name":"Hainan Medical University","award":["XRC202029"],"award-info":[{"award-number":["XRC202029"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Infections caused by Candida albicans (C. albicans) and increasing resistance to commonly used drugs lead to a variety of mucosal diseases and systemic infectious diseases. We previously confirmed that the essential oil of Clausena lansium (Lour.) Skeels seeds (CSEO) had antifungal activity against C. albicans, but the detailed mechanism between the chemical components and antifungal activity is unclear. In this study, a quantitative analysis of five volatile components of CSEO, including sabinene, \u03b1-phellandrene, \u03b2-phellandrene, 4-terpineol, and \u03b2-caryophyllene, was carried out using the gas chromatography\u2013mass spectrometry (GC\u2013MS) method. Both the broth dilution and kinetic growth methods proved that the antifungal activity of CSEO against fluconazole-resistant C. albicans was better than that of its main components (sabinene and 4-terpineol). To further investigate the inhibitory mechanism, the transcriptional responses of C. albicans to CSEO, sabinene, and 4-terpineol treatment were determined based on RNA-seq. The Venn diagram and clustering analysis pattern of differential expression genes showed the mechanism of CSEO and 4-terpineol\u2019s anti-C. albicans activity might be similar from the perspective of the genes. Functional enrichment analysis suggested that CSEO regulated adherence-, hyphae-, and biofilm-formation-related genes, which may be CSEO\u2019s active mechanism of inhibiting the growth of fluconazole-resistant C. albicans. Overall, we preliminarily revealed the molecular mechanism between the chemical components and the antifungal activity of CSEO against C. albicans. This study provides new insights to overcome the azole resistance of C. albicans and promote the development and application of C. lansium (Lour.) Skeels seeds.<\/jats:p>","DOI":"10.3390\/molecules28248052","type":"journal-article","created":{"date-parts":[[2023,12,12]],"date-time":"2023-12-12T05:23:22Z","timestamp":1702358602000},"page":"8052","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["RNA-Seq-Based Transcriptomics and GC\u2013MS Quantitative Analysis Reveal Antifungal Mechanisms of Essential Oil of Clausena lansium (Lour.) Skeels Seeds against Candida albicans"],"prefix":"10.3390","volume":"28","author":[{"given":"Yinzheng","family":"Ma","sequence":"first","affiliation":[{"name":"Public Research Center, Hainan Medical University, Haikou 571199, China"},{"name":"School of Pharmacy, Hainan Medical University, Haikou 571199, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinlei","family":"Sui","sequence":"additional","affiliation":[{"name":"Public Research Center, Hainan Medical University, Haikou 571199, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Wang","sequence":"additional","affiliation":[{"name":"Public Research Center, Hainan Medical University, Haikou 571199, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wanying","family":"Sun","sequence":"additional","affiliation":[{"name":"Public Research Center, Hainan Medical University, Haikou 571199, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guohui","family":"Yi","sequence":"additional","affiliation":[{"name":"Public Research Center, Hainan Medical University, Haikou 571199, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3045-1323","authenticated-orcid":false,"given":"Jinyan","family":"Wu","sequence":"additional","affiliation":[{"name":"Public Research Center, Hainan Medical University, Haikou 571199, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shi","family":"Qiu","sequence":"additional","affiliation":[{"name":"Public Research Center, Hainan Medical University, Haikou 571199, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lili","family":"Wang","sequence":"additional","affiliation":[{"name":"Public Research Center, Hainan Medical University, Haikou 571199, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aihua","family":"Zhang","sequence":"additional","affiliation":[{"name":"Public Research Center, Hainan Medical University, Haikou 571199, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaowen","family":"He","sequence":"additional","affiliation":[{"name":"Public Research Center, Hainan Medical University, Haikou 571199, China"},{"name":"School of Pharmacy, Hainan Medical University, Haikou 571199, China"},{"name":"Key Laboratory of Emergency and Trauma of Ministry of Education, Research Unit of Island, Emergency Medicine of Chinese Academy of Medical Sciences, Hainan Medical University, Haikou 571199, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,12,12]]},"reference":[{"key":"ref_1","first-page":"189","article-title":"Candida albicans the main opportunistic pathogenic fungus in humans","volume":"55","year":"2022","journal-title":"Rev. Argent. Microbiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"e036452","DOI":"10.1136\/bmjopen-2019-036452","article-title":"Risk of invasive candidiasis with prolonged duration of ICU stay: A systematic review and meta-analysis","volume":"10","author":"Zhang","year":"2020","journal-title":"BMJ Open"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"740","DOI":"10.1016\/j.jmii.2022.03.001","article-title":"Risk factors and prognoses of invasive Candida infection in surgical critical ill patients with perforated peptic ulcer","volume":"55","author":"Li","year":"2022","journal-title":"J. Microbiol. Immun. Infect."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Talapko, J., Juzba\u0161i\u0107, M., Matijevi\u0107, T., Pustijanac, E., Beki\u0107, S., Kotris, I., and \u0160krlec, I. (2021). Candida albicans- the virulence factors and clinical manifestations of infection. J. Fungi, 7.","DOI":"10.3390\/jof7020079"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"792","DOI":"10.1016\/j.cmi.2019.03.028","article-title":"Resistance of Candida to azoles and echinocandins worldwide","volume":"25","author":"Pristov","year":"2019","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"116","DOI":"10.2174\/1389450122666210719124143","article-title":"Antimycotic drugs and their mechanisms of resistance to Candida species","volume":"23","author":"Dahiya","year":"2022","journal-title":"Curr. Drug Targets"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"103953","DOI":"10.1016\/j.isci.2022.103953","article-title":"The role of Candida albicans stress response pathways in antifungal tolerance and resistance","volume":"25","author":"Iyer","year":"2022","journal-title":"iScience"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Wang, Y., Lu, C., Zhao, X., Wang, D., Liu, Y., and Sun, S. (2021). Antifungal activity and potential mechanism of Asiatic acid alone and in combination with fluconazole against Candida albicans. Biomed. Pharmacother., 139.","DOI":"10.1016\/j.biopha.2021.111568"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1016\/j.foodchem.2011.09.012","article-title":"Antifungal activity of the honey flavonoid extract against Candida albicans","volume":"131","author":"Candiracci","year":"2012","journal-title":"Food Chem."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"113005","DOI":"10.1016\/j.indcrop.2020.113005","article-title":"Chemotypes of Juniperus oxycedrus in Bulgaria and the antimicrobial activity of galbuli essential oils","volume":"158","author":"Semerdjieva","year":"2020","journal-title":"Ind. Crop. Prod."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"111352","DOI":"10.1016\/j.lwt.2021.111352","article-title":"Antimicrobial combined action of Mentha pulegium, Ormenis mixta and Mentha piperita essential oils against S. aureus, E. coli and C. tropicalis: Application of mixture design methodology","volume":"145","author":"Chraibi","year":"2021","journal-title":"LWT-Food Sci. Technol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1080\/13102818.2015.1020341","article-title":"Chemical composition and antifungal activity of essential oil of Hyssopus officinalis L. from Bulgaria against clinical isolates of Candida species","volume":"29","author":"Hristova","year":"2015","journal-title":"Biotechnol. Biotechnol. Equip."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"100911","DOI":"10.1016\/j.mycmed.2019.100911","article-title":"Thymus vulgaris essential oil and thymol inhibit biofilms and interact synergistically with antifungal drugs against drug resistant strains of Candida albicans and Candida tropicalis","volume":"30","author":"Jafri","year":"2020","journal-title":"J. Mycol. Med."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"871","DOI":"10.1007\/978-94-007-4053-2_100","article-title":"Clausena lansium","volume":"Volume 4","author":"Lim","year":"2012","journal-title":"Edible Medicinal and Non-Medicinal Plants"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1355","DOI":"10.1002\/ps.6150","article-title":"Chemical components from the stems and leaves of Clausena lansium Lour. Skeels and their potential herbicidal effects","volume":"77","author":"Peng","year":"2021","journal-title":"Pest Manag. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"e07003","DOI":"10.1016\/j.heliyon.2021.e07003","article-title":"Cognition enhancing effects of Clausena lansium (Lour.) peel extract attenuate chronic restraint stress-induced memory deficit in rats","volume":"7","author":"Phachonpai","year":"2021","journal-title":"Heliyon"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1016\/j.indcrop.2018.05.037","article-title":"Bioassay-guided isolation of antifungal amides against Sclerotinia sclerotiorum from the seeds of Clausena lansium","volume":"121","author":"Yan","year":"2018","journal-title":"Ind. Crop. Prod."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"106673","DOI":"10.1016\/j.ijantimicag.2022.106673","article-title":"Candida albicans biofilms: Antifungal resistance, immune evasion, and emerging therapeutic strategies","volume":"60","author":"Fan","year":"2022","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1016\/j.jff.2018.05.057","article-title":"Water soluble fraction from ethanolic extract of Clausena lansium seeds alleviates obesity and insulin resistance and changes the composition of gut microbiota in high-fat diet-fed mice","volume":"47","author":"Shen","year":"2018","journal-title":"J. Funct. Foods"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1515\/znc-2004-3-401","article-title":"Chemical composition of the essential oils of Clausena lansium from Hainan island, China","volume":"59","author":"Zhao","year":"2004","journal-title":"Z. Naturforsch. C"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"19666","DOI":"10.1038\/s41598-021-99188-x","article-title":"The influence of the chemical composition of essential oils of Clausena lansium seeds on the growth of Candida strains","volume":"11","author":"Ma","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"He, X., Ma, Y., Yi, G., Wen, Y., Zhang, Y., Zhang, H., and Zhang, L. (2019). Rapid and sensitive analysis of volatile components of different parts of Clausena lansium by ionic liquid based headspace gas chromatography-mass spectrometry. Molecules, 24.","DOI":"10.3390\/molecules24010091"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/j.tim.2016.09.004","article-title":"Portrait of Candida species biofilm regulatory network genes","volume":"25","author":"Henriques","year":"2017","journal-title":"Trends Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.mycmed.2018.12.003","article-title":"Activity of coumarin against Candida albicans biofilms","volume":"29","author":"Xu","year":"2018","journal-title":"J. Mycol. Med."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.fitote.2018.04.023","article-title":"Nematicidal amide alkaloids from the seeds of Clausena lansium","volume":"128","author":"Fan","year":"2017","journal-title":"Fitoterapia"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/S0966-842X(01)01984-9","article-title":"The ALS gene family of Candida albicans","volume":"9","author":"Hoyer","year":"2001","journal-title":"Trends Microbiol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1038\/nrmicro2475","article-title":"Genetic control of Candida albicans biofilm development","volume":"9","author":"Finkel","year":"2011","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1447","DOI":"10.1007\/s10103-018-2487-8","article-title":"Photodynamic inactivation in the expression of the Candida albicans genes ALS3, HWP1, BCR1, TEC1, CPH1, and EFG1 in biofilms","volume":"33","author":"Fernanda","year":"2018","journal-title":"Laser Med. Sci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.micpath.2014.03.003","article-title":"Impact of Candida albicans hyphal wall protein 1 (HWP1) genotype on biofilm production and fungal susceptibility to microglial cells","volume":"69\u201370","author":"Orsi","year":"2014","journal-title":"Microb. Pathog."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"400","DOI":"10.1128\/MMBR.67.3.400-428.2003","article-title":"Candida albicans secreted aspartyl proteinases in virulence and pathogenesis","volume":"67","author":"Naglik","year":"2003","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1309","DOI":"10.1016\/j.bbadis.2016.04.004","article-title":"The adaptive metabolic response involves specific protein glutathionylation during the filamentation process in the pathogen Candida albicans","volume":"1862","author":"Gergondey","year":"2016","journal-title":"BBA\u2014Mol. Basis Dis."},{"key":"ref_32","unstructured":"(2008). Reference Method for Broth Dilution Antifungal Susceptibility Testing of Filamentous Fungi; Approved Standard. Standard No. CLSI Document M38-A2."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/28\/24\/8052\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T21:37:17Z","timestamp":1760132237000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/28\/24\/8052"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,12,12]]},"references-count":32,"journal-issue":{"issue":"24","published-online":{"date-parts":[[2023,12]]}},"alternative-id":["molecules28248052"],"URL":"https:\/\/doi.org\/10.3390\/molecules28248052","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,12,12]]}}}