{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T01:57:19Z","timestamp":1788573439218,"version":"build-2803163510"},"reference-count":104,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2023,12,13]],"date-time":"2023-12-13T00:00:00Z","timestamp":1702425600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"project Wallonie-Bruxelles\/China (MOST)","award":["N\u00b0 CDR J.0058.21"],"award-info":[{"award-number":["N\u00b0 CDR J.0058.21"]}]},{"name":"project Wallonie-Bruxelles\/China (MOST)","award":["2021YFE0194000"],"award-info":[{"award-number":["2021YFE0194000"]}]},{"name":"Fonds pour la Recherche Scientifique FNRS","award":["N\u00b0 CDR J.0058.21"],"award-info":[{"award-number":["N\u00b0 CDR J.0058.21"]}]},{"name":"Fonds pour la Recherche Scientifique FNRS","award":["2021YFE0194000"],"award-info":[{"award-number":["2021YFE0194000"]}]},{"name":"National Key R&amp;D Program of China","award":["N\u00b0 CDR J.0058.21"],"award-info":[{"award-number":["N\u00b0 CDR J.0058.21"]}]},{"name":"National Key R&amp;D Program of China","award":["2021YFE0194000"],"award-info":[{"award-number":["2021YFE0194000"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>The interaction between SARS-CoV-2 spike RBD and ACE2 proteins is a crucial step for host cell infection by the virus. Without it, the entire virion entrance mechanism is compromised. The aim of this study was to evaluate the capacity of various natural product classes, including flavonoids, anthraquinones, saponins, ivermectin, chloroquine, and erythromycin, to modulate this interaction. To accomplish this, we applied a recently developed a microfluidic diffusional sizing (MDS) technique that allows us to probe protein-protein interactions via measurements of the hydrodynamic radius (Rh) and dissociation constant (KD); the evolution of Rh is monitored in the presence of increasing concentrations of the partner protein (ACE2); and the KD is determined through a binding curve experimental design. In a second time, with the protein partners present in equimolar amounts, the Rh of the protein complex was measured in the presence of different natural products. Five of the nine natural products\/extracts tested were found to modulate the formation of the protein complex. A methanol extract of Chenopodium quinoa Willd bitter seed husks (50 \u00b5g\/mL; bisdesmoside saponins) and the flavonoid naringenin (1 \u00b5M) were particularly effective. This rapid selection of effective modulators will allow us to better understand agents that may prevent SARS-CoV-2 infection.<\/jats:p>","DOI":"10.3390\/molecules28248072","type":"journal-article","created":{"date-parts":[[2023,12,13]],"date-time":"2023-12-13T12:00:37Z","timestamp":1702468837000},"page":"8072","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Microfluidic Diffusion Sizing Applied to the Study of Natural Products and Extracts That Modulate the SARS-CoV-2 Spike RBD\/ACE2 Interaction"],"prefix":"10.3390","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7997-7054","authenticated-orcid":false,"given":"Jason","family":"Fauquet","sequence":"first","affiliation":[{"name":"Unit of Therapeutic Chemistry and Pharmacognosy, University of Mons (UMONS), 7000 Mons, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-2264-4193","authenticated-orcid":false,"given":"Julie","family":"Carette","sequence":"additional","affiliation":[{"name":"Unit of Therapeutic Chemistry and Pharmacognosy, University of Mons (UMONS), 7000 Mons, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0484-1478","authenticated-orcid":false,"given":"Pierre","family":"Duez","sequence":"additional","affiliation":[{"name":"Unit of Therapeutic Chemistry and Pharmacognosy, University of Mons (UMONS), 7000 Mons, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1745-846X","authenticated-orcid":false,"given":"Jiuliang","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8697-2809","authenticated-orcid":false,"given":"Amandine","family":"Nachtergael","sequence":"additional","affiliation":[{"name":"Unit of Therapeutic Chemistry and Pharmacognosy, University of Mons (UMONS), 7000 Mons, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,12,13]]},"reference":[{"key":"ref_1","unstructured":"WHO (2023, October 21). 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