{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T07:58:38Z","timestamp":1790409518789,"version":"4.1.0"},"reference-count":44,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2023,12,20]],"date-time":"2023-12-20T00:00:00Z","timestamp":1703030400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China","award":["81960836"],"award-info":[{"award-number":["81960836"]}]},{"name":"National Natural Science Foundation of China","award":["NGY2020045"],"award-info":[{"award-number":["NGY2020045"]}]},{"name":"National Natural Science Foundation of China","award":["2020AAC03126"],"award-info":[{"award-number":["2020AAC03126"]}]},{"name":"Ningxia Higher Education Scientific Research Project","award":["81960836"],"award-info":[{"award-number":["81960836"]}]},{"name":"Ningxia Higher Education Scientific Research Project","award":["NGY2020045"],"award-info":[{"award-number":["NGY2020045"]}]},{"name":"Ningxia Higher Education Scientific Research Project","award":["2020AAC03126"],"award-info":[{"award-number":["2020AAC03126"]}]},{"name":"Ningxia Natural Science Foundation","award":["81960836"],"award-info":[{"award-number":["81960836"]}]},{"name":"Ningxia Natural Science Foundation","award":["NGY2020045"],"award-info":[{"award-number":["NGY2020045"]}]},{"name":"Ningxia Natural Science Foundation","award":["2020AAC03126"],"award-info":[{"award-number":["2020AAC03126"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Lycium barbarum polysaccharide (LBP) is the main active component of Fructus Lycii, exhibiting various biological activities. This study aims to explore the protective effects of LBP on human corneal epithelial cells (HCEC) and a rat corneal injury model. Potential target points for LBP improving corneal injury repair were screened from public databases, and functional and pathway enrichment analyses of core targets were conducted using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). Rat corneal alkali burns and HCEC oxidative stress injury models were established, and the results were validated through slit lamp examination, HE staining, TUNEL assay, immunofluorescence, CCK-8 assay, flow cytometry, scratch assay, and qRT-PCR methods. In the context of database retrieval, identification of 10 LBP monosaccharide components and 50 corneal injury repair-related targets was achieved. KEGG pathway analysis suggested that LBP might regulate the IL-17 and TNF signaling pathways through targets such as JUN, CASP3, and MMP9, thereby improving corneal damage. In vivo and in vitro experimental results indicated that LBP could reduce the increase of inflammation index scores (p &lt; 0.05), inflammatory cell density (p &lt; 0.01), TUNEL-positive cells (p &lt; 0.01), corneal opacity scores (p &lt; 0.01), and expression of corneal stromal fibrosis-related proteins \u03b1-SMA, FN, and COL (p &lt; 0.01) caused by chemical damage to rat corneas. LBP inhibited oxidative stress-induced decreases in cell viability (p &lt; 0.001) and migration healing ability (p &lt; 0.01) in HCECs, reducing apoptosis rates (p &lt; 0.001), ROS levels (p &lt; 0.001), and the expression of inflammatory factors TNF-\u03b1 and IL-6 (p &lt; 0.01). qRT-PCR results demonstrated that LBP intervention decreased the mRNA levels of JUN, CASP3, and MMP9 in H2O2-induced alkaline-burned corneas and HCECs (p &lt; 0.01).The integrated results from network pharmacology and validation experiments suggest that the inhibitory effects of LBP on apoptosis, inflammation, and fibrosis after corneal injury may be achieved through the suppression of the TNF and IL-17 signaling pathways mediated by JUN, CASP3, and MMP9.<\/jats:p>","DOI":"10.3390\/molecules29010049","type":"journal-article","created":{"date-parts":[[2023,12,20]],"date-time":"2023-12-20T11:24:33Z","timestamp":1703071473000},"page":"49","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Exploring the Role of Lycium barbarum Polysaccharide in Corneal Injury Repair and Investigating the Relevant Mechanisms through In Vivo and In Vitro Experiments"],"prefix":"10.3390","volume":"29","author":[{"given":"Qian","family":"Liu","sequence":"first","affiliation":[{"name":"Key Laboratory of Ningxia Ethnomedicine Modernization, Ministry of Education, Ningxia Medical University, Yinchuan 750004, China"},{"name":"College of Clinical Medicine, Ningxia Medical University, Yinchuan 750004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Nan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Ningxia Ethnomedicine Modernization, Ministry of Education, Ningxia Medical University, Yinchuan 750004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yifan","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Traditional Chinese Medicine, Ningxia Medical University, Yinchuan 750004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangyang","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Ningxia Ethnomedicine Modernization, Ministry of Education, Ningxia Medical University, Yinchuan 750004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjie","family":"Jiang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Ningxia Ethnomedicine Modernization, Ministry of Education, Ningxia Medical University, Yinchuan 750004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taiqiang","family":"Jiao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Ningxia Ethnomedicine Modernization, Ministry of Education, Ningxia Medical University, Yinchuan 750004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaqing","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Ningxia Ethnomedicine Modernization, Ministry of Education, Ningxia Medical University, Yinchuan 750004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-8116-1357","authenticated-orcid":false,"given":"Xusheng","family":"Jia","sequence":"additional","affiliation":[{"name":"Key Laboratory of Ningxia Ethnomedicine Modernization, Ministry of Education, Ningxia Medical University, Yinchuan 750004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengyi","family":"Ye","sequence":"additional","affiliation":[{"name":"School of Traditional Chinese Medicine, Ningxia Medical University, Yinchuan 750004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Niu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Ningxia Ethnomedicine Modernization, Ministry of Education, Ningxia Medical University, Yinchuan 750004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2838-0976","authenticated-orcid":false,"given":"Ling","family":"Yuan","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Ningxia Medical University, Yinchuan 750004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,12,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Du, S., Han, B., Li, K., Zhang, X., Sha, X., and Gao, L. 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