{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T17:00:30Z","timestamp":1792170030782,"version":"4.4.0"},"reference-count":50,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2024,1,5]],"date-time":"2024-01-05T00:00:00Z","timestamp":1704412800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Polish Ministry of Science and Higher Education"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Presently, there is an increasing shift towards the utilization of natural antioxidants and compounds with protective attributes for fatty acids in order to replace synthetic counterparts that may pose health risks. This transition aligns with the growing emphasis on promoting healthy and organic food choices. Essential oils stand out in this context due to scientific validations of their antioxidant properties. There are few published research results concerning changes in the fatty acid composition in model systems with the addition of essential oils. This study aims to investigate the impact of incorporating peppermint and thyme oils on inhibiting changes in the fatty acid profile of sunflower oil stored at both room temperature with exposure to daylight and in a thermostat set at 40 \u00b0C. The experimental procedure involved the addition of peppermint and thyme oils, along with butylated hydroxyanisole (BHA), to batches of sunflower oil. The samples were then stored for 11 months. The study observed a detrimental influence of storage conditions on the quantitative changes in the fatty acid profile of the sunflower oil. The addition of BHA stabilized the content of linoleic acid in the sunflower oil (approximately 53 g\/100 g of linoleic acid compared to approximately 58 g\/100 g in the control sample). Meanwhile, the model system of sunflower oil with the addition of peppermint and thyme oils (40 \u00b0C) exhibited a statistically significant decrease in the concentration of linoleic acid to approximately 8 g\/100 g after eleven months of thermostating. Similar trends to those observed for linoleic acid were noted for the total fatty acid content in the sunflower oil. Notably, the efficacy of the selected substances in inhibiting adverse transformations in fats was contingent upon their concentration and the storage temperature.<\/jats:p>","DOI":"10.3390\/molecules29020292","type":"journal-article","created":{"date-parts":[[2024,1,5]],"date-time":"2024-01-05T07:38:31Z","timestamp":1704440311000},"page":"292","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["The Effect of Peppermint and Thyme Oils on Stabilizing the Fatty Acid Profile of Sunflower Oil"],"prefix":"10.3390","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3063-8710","authenticated-orcid":false,"given":"Rados\u0142aw","family":"Kowalski","sequence":"first","affiliation":[{"name":"Department of Analysis and Food Quality Assessment, University of Life Sciences in Lublin, 8 Skromna Str., 20-704 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1872-2381","authenticated-orcid":false,"given":"Gra\u017cyna","family":"Kowalska","sequence":"additional","affiliation":[{"name":"Department of Tourism and Recreation, University of Life Sciences in Lublin, 15 Akademicka Str., 20-950 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2021-4490","authenticated-orcid":false,"given":"Przemys\u0142aw","family":"Mitura","sequence":"additional","affiliation":[{"name":"Department of Urology and Oncological Urology, Medical University of Lublin, 8 Jaczewskiego Str., 20-954 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafa\u0142","family":"Rowi\u0144ski","sequence":"additional","affiliation":[{"name":"Department of Tourism and Recreation, University of Life Sciences in Lublin, 15 Akademicka Str., 20-950 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Urszula","family":"Pankiewicz","sequence":"additional","affiliation":[{"name":"Department of Analysis and Food Quality Assessment, University of Life Sciences in Lublin, 8 Skromna Str., 20-704 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna","family":"Hawlena","sequence":"additional","affiliation":[{"name":"Department of Tourism and Recreation, University of Life Sciences in Lublin, 15 Akademicka Str., 20-950 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,1,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Billingsley, H., Carbone, S., and Lavie, C. (2018). Dietary Fats and Chronic Noncommunicable Diseases. Nutrients, 10.","DOI":"10.3390\/nu10101385"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"113416","DOI":"10.1016\/j.lwt.2022.113416","article-title":"Improving the Lipid Profile of Beef Burgers Added with Chia Oil (Salvia hispanica L.) or Hemp Oil (Cannabis sativa L.) Gelled Emulsions as Partial Animal Fat Replacers","volume":"161","year":"2022","journal-title":"LWT"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Shahidi, F., and Hossain, A. (2022). Role of Lipids in Food Flavor Generation. Molecules, 27.","DOI":"10.3390\/molecules27155014"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Temkov, M., and Mure\u0219an, V. (2021). Tailoring the Structure of Lipids, Oleogels and Fat Replacers by Different Approaches for Solving the Trans-Fat Issue\u2014A Review. Foods, 10.","DOI":"10.3390\/foods10061376"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"L\u00f6we, J., and Gr\u00f6ger, H. (2020). Fatty Acid Hydratases: Versatile Catalysts to Access Hydroxy Fatty Acids in Efficient Syntheses of Industrial Interest. Catalysts, 10.","DOI":"10.3390\/catal10030287"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/j.lwt.2019.04.016","article-title":"The Effect of an Addition of Marjoram Oil on Stabilization Fatty Acids Profile of Rapeseed Oil","volume":"109","author":"Kowalski","year":"2019","journal-title":"LWT"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1016\/j.foodchem.2004.03.011","article-title":"Influence of Heating on Antioxidant Activity and the Chemical Composition of Some Spice Essential Oils","volume":"89","author":"Tomaino","year":"2005","journal-title":"Food Chem."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"792","DOI":"10.1007\/BF02653670","article-title":"Antioxidant Activity of Some Spice Essential Oils on Linoleic Acid Oxidation in Aqueous Media","volume":"66","author":"Farag","year":"1989","journal-title":"J. Am. Oil Chem. Soc."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1016\/j.foodqual.2003.08.001","article-title":"Olive Oil Flavoured by the Essential Oils of Mentha \u00d7 Piperita and Thymus mastichina L.","volume":"15","author":"Neves","year":"2004","journal-title":"Food Qual. Prefer."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.lwt.2018.04.045","article-title":"Effect of the Method of Rapeseed Oil Aromatisation with Rosemary Rosmarinus officinalis L. on the Content of Volatile Fraction","volume":"95","author":"Kowalski","year":"2018","journal-title":"LWT"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1138","DOI":"10.1007\/s13197-019-04149-y","article-title":"Effect of Rapeseed Oil Aromatisation with Marjoram on the Content of Volatile Fraction and Antioxidant Properties","volume":"57","author":"Kowalski","year":"2020","journal-title":"J. Food Sci. Technol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"877","DOI":"10.1007\/s11694-017-9702-3","article-title":"Effect of Storage on Oxidation Stability of Essential Oils Derived from Culinary Herbs and Spices","volume":"12","author":"Naeem","year":"2018","journal-title":"J. Food Meas. Charact."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Popa, C.L., Lupitu, A., Mot, M.D., Copolovici, L., Moisa, C., and Copolovici, D.M. (2021). Chemical and Biochemical Characterization of Essential Oils and Their Corresponding Hydrolats from Six Species of the Lamiaceae Family. Plants, 10.","DOI":"10.3390\/plants10112489"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Morkeli\u016bn\u0117, A., Rasiukevi\u010di\u016bt\u0117, N., \u0160ernait\u0117, L., and Valiu\u0161kait\u0117, A. (2021). The Use of Essential Oils from Thyme, Sage and Peppermint against Colletotrichum acutatum. Plants, 10.","DOI":"10.3390\/plants10010114"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1707","DOI":"10.1016\/j.foodcont.2011.04.002","article-title":"Antimicrobial Potential and Chemical Composition of Mentha piperita Oil in Liquid and Vapour Phase against Food Spoiling Microorganisms","volume":"22","author":"Tyagi","year":"2011","journal-title":"Food Control"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1021\/ed085p133","article-title":"Analysis of Peppermint Leaf and Spearmint Leaf Extracts by Thin-Layer Chromatography","volume":"85","author":"Pelter","year":"2008","journal-title":"J. Chem. Educ."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1002\/ptr.1936","article-title":"A Review of the Bioactivity and Potential Health Benefits of Peppermint Tea (Mentha piperita L.)","volume":"20","author":"McKay","year":"2006","journal-title":"Phytother. Res."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"251","DOI":"10.25121\/PF.2017.18.4.251","article-title":"Sk\u0142ad Chemiczny i Dzia\u0142anie Przeciwutleniaj\u0105ce Ekstrakt\u00f3w z Mentha \u00d7 Piperita L.","volume":"18","author":"Najda","year":"2017","journal-title":"Post\u0119py Fitoter."},{"key":"ref_19","first-page":"241","article-title":"Analysis of Chemical Composition of Essential Oil in the Herb of Thyme (Thymus vulgaris L.) Grown in South-Eastern Poland","volume":"53","year":"2007","journal-title":"Herba Pol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"832","DOI":"10.1080\/0972060X.2015.1025296","article-title":"Comparison of the Chemical Composition of Essential Oils Isolated by Water-Steam Distillation and Hydrodistillation from Garden Thyme (Thymus vulgaris L.)","volume":"19","author":"Wesolowska","year":"2016","journal-title":"J. Essent. Oil Bear. Plants"},{"key":"ref_21","first-page":"10","article-title":"Comparative Chemical Composition of the Essential Oil of Thymus vulgaris L. from Different Geographical Sources","volume":"51","author":"Raal","year":"2005","journal-title":"Herba Pol."},{"key":"ref_22","first-page":"93","article-title":"Effects of Natural Phenolics and Synthetic Antioxidants on the Oxidative Thermal Stability of Refined and Purified Sunflower Oils","volume":"98","author":"Yildiz","year":"2021","journal-title":"Riv. Ital. Delle Sostanze Grasse"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1007\/s11694-020-00665-0","article-title":"Antioxidant Properties of Thymol, Carvacrol, and Thymoquinone and Its Efficiencies on the Stabilization of Refined and Stripped Corn Oils","volume":"15","author":"Yildiz","year":"2021","journal-title":"J. Food Meas. Charact."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1111\/j.1745-4557.2010.00295.x","article-title":"Changes of Linoleic Acid Concentration during Heating of Some Plant-Origin Oils with Polyphenol Addition","volume":"33","author":"Kowalski","year":"2010","journal-title":"J. Food Qual."},{"key":"ref_25","first-page":"15","article-title":"GC Analysis of Changes in the Fatty Acid Composition of Sunflower and Olive Oils Heated with Quercetin, Caffeic Acid, Protocatechuic Acid, and Butylated Hydroxyanisole","volume":"18","author":"Kowalski","year":"2007","journal-title":"Acta Chromatogr."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"820","DOI":"10.1016\/j.foodchem.2008.06.038","article-title":"Silphium L. Extracts\u2014Composition and Protective Effect on Fatty Acids Content in Sunflower Oil Subjected to Heating and Storage","volume":"112","author":"Kowalski","year":"2009","journal-title":"Food Chem."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"295","DOI":"10.5604\/12338273.1058134","article-title":"Zmiany Liczby Kwasowej i Nadtlenkowej T\u0142uszczu Produkt\u00f3w Rzepakowych Przechowywanych w R\u00f3\u017cnych Warunkach Bez i z Dodatkiem Przeciwutleniacza","volume":"33","author":"Milczarek","year":"2013","journal-title":"Ro\u015bliny Oleiste\u2014Oilseed Crops"},{"key":"ref_28","unstructured":"(2011). Animal and Vegetable Fats and Oils\u2014Gas Chromatography of Fatty Acid Methyl Esters\u2014Part 2: Preparation of Methyl Esters of Fatty Acids (Standard No. PN-EN ISO 12966-2:2011)."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1016\/j.foodchem.2004.04.037","article-title":"Heating Effects on Some Quality Characteristics of Date Seed Oil","volume":"91","author":"Besbes","year":"2005","journal-title":"Food Chem."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1007\/BF02666044","article-title":"Frying Performance of Palm Oil Liquid Fractions","volume":"58","author":"Bracco","year":"1981","journal-title":"J. Am. Oil Chem. Soc."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1102","DOI":"10.1007\/BF02636232","article-title":"Quality Control in the Use of Deep Frying Oils","volume":"61","author":"Stevenson","year":"1984","journal-title":"J. Am. Oil Chem. Soc."},{"key":"ref_32","unstructured":"Halilovi\u0107, B., \u010corbo, S., Gavri\u0107, T., and Begi\u0107, M. (2023). Lecture Notes in Bioengineering: Scientific-Expert Conference of Agriculture and Food Industry, Proceedings of the 32nd Scientific-Expert Conference of Agricultureand Food Industry, Sarajevo, Bosnia and Herzegovina, 1\u20132 December 2022, Springer."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Mici\u0107, D., \u0110urovi\u0107, S., Riabov, P., Tomi\u0107, A., \u0160ovljanski, O., Filip, S., Tosti, T., Doj\u010dinovi\u0107, B., Bo\u017eovi\u0107, R., and Jovanovi\u0107, D. (2021). Rosemary Essential Oils as a Promising Source of Bioactive Compounds: Chemical Composition, Thermal Properties, Biological Activity, and Gastronomical Perspectives. Foods, 10.","DOI":"10.3390\/foods10112734"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"012007","DOI":"10.1088\/1755-1315\/209\/1\/012007","article-title":"The Influence of Essential Oil Addition to Oxidative Stability of Palmoil Biodisel","volume":"209","author":"Setyaningsih","year":"2018","journal-title":"IOP Conf. Ser. Earth Environ. Sci."},{"key":"ref_35","unstructured":"G\u00f3ra, J., and Lis, A. (2017). The Most Valuable Essential Oils I, Wydawnictwo Politechniki \u0141\u00f3dzkiej."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1111\/j.1745-4565.2011.00341.x","article-title":"Composition, Antifungal Activity and Main Fungitoxic Components of the Essential Oil of Mentha piperita L.","volume":"32","author":"Freire","year":"2012","journal-title":"J. Food Saf."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1016\/S0021-9673(01)80947-X","article-title":"A Generalization of the Retention Index System Including Linear Temperature Programmed Gas\u2014Liquid Partition Chromatography","volume":"11","author":"Kratz","year":"1963","journal-title":"J. Chromatogr. A"},{"key":"ref_38","unstructured":"Adams, R.P. (2001). Identification of Essential Oil Compounds by Gas Chromatography\/Quadrupole Mass Spectroscopy, Allured."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Gavrila, A.I., Chisega-Negrila, C.G., Maholea, L., Gavrila, M.L., Parvulescu, O.C., and Popa, I. (2023). Enhancing the Extraction Process Efficiency of Thyme Essential Oil by Combined Ultrasound and Microwave Techniques. Agronomy, 13.","DOI":"10.20944\/preprints202308.1521.v1"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Radivojac, A., Bera, O., Zekovi\u0107, Z., Tesli\u0107, N., Mrkonji\u0107, \u017d., Bursa\u0107 Kova\u010devi\u0107, D., Putnik, P., and Pavli\u0107, B. (2021). Extraction of Peppermint Essential Oils and Lipophilic Compounds: Assessment of Process Kinetics and Environmental Impacts with Multiple Techniques. Molecules, 26.","DOI":"10.3390\/molecules26102879"},{"key":"ref_41","first-page":"130","article-title":"In-Vitro Antioxidant Activity and GC\/MS Studies on the Leaves of Mentha piperita (Lamiaceae) from Morocco","volume":"3","author":"Derwich","year":"2011","journal-title":"Int. J. Pharm. Sci. Drug Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/S0308-8146(98)00086-7","article-title":"Antioxidant Activity and Mechanism of Action of Thymol and Carvacrol in Two Lipid Systems","volume":"64","author":"Yanishlieva","year":"1999","journal-title":"Food Chem."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Kiralan, M., Ula\u015f, M., \u00d6zaydin, A., \u00d6zdem\u0131r, N., \u00d6zkan, G., Bayrak, A., and Ramadan, M.F. (2017). Blends of Cold Pressed Black Cumin Oil and Sunflower Oil with Improved Stability: A Study Based on Changes in the Levels of Volatiles, Tocopherols and Thymoquinone during Accelerated Oxidation Conditions. J. Food Biochem., 41.","DOI":"10.1111\/jfbc.12272"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1111\/1541-4337.12006","article-title":"Stability of Essential Oils: A Review","volume":"12","author":"Turek","year":"2013","journal-title":"Compr. Rev. Food Sci. Food Saf."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"796","DOI":"10.1021\/jf00039a039","article-title":"Studies on the Storage Quality of Carbon Dioxide-Extracted Cardamom and Clove Bud Oils","volume":"42","author":"Gopalakrishnan","year":"1994","journal-title":"J. Agric. Food Chem."},{"key":"ref_46","first-page":"48","article-title":"Quality Criteria of Bitter Fennel Oil in the German Pharmacopoeia","volume":"9","author":"Braun","year":"1999","journal-title":"Pharm. Pharmacol. Lett."},{"key":"ref_47","first-page":"36","article-title":"Przeciwutleniacze w \u017bywno\u015bci","volume":"62","year":"2008","journal-title":"Przemys\u0142 Spo\u017cywczy"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1723","DOI":"10.1111\/j.1745-4514.2010.00497.x","article-title":"Herb Extract Influence on the Oxidative Stability of Selected Lipids","volume":"35","author":"Sidor","year":"2011","journal-title":"J. Food Biochem."},{"key":"ref_49","unstructured":"(2008). Polish Pharmacopoeia VIII, PTFarm; Polish Pharmaceutical Society."},{"key":"ref_50","unstructured":"(2015). Animal and Vegetable Fats and Oils\u2014Gas Chromatography of Fatty Acid Methyl Esters\u2014Part 1: Guidelines on Modern Gas Chromatography of Fatty Acid Methyl Esters (Standard No. PN-EN ISO 12966-1:2015-01)."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/29\/2\/292\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T13:40:51Z","timestamp":1760103651000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/29\/2\/292"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1,5]]},"references-count":50,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2024,1]]}},"alternative-id":["molecules29020292"],"URL":"https:\/\/doi.org\/10.3390\/molecules29020292","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,1,5]]}}}