{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T14:07:57Z","timestamp":1780668477144,"version":"3.54.1"},"reference-count":33,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2024,1,13]],"date-time":"2024-01-13T00:00:00Z","timestamp":1705104000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Science Program of Hunan Administration of Traditional Chinese Medicine, China","award":["C2022043"],"award-info":[{"award-number":["C2022043"]}]},{"name":"Science Program of Hunan Administration of Traditional Chinese Medicine, China","award":["A2022005-4"],"award-info":[{"award-number":["A2022005-4"]}]},{"name":"Science Program of Hunan Administration of Traditional Chinese Medicine, China","award":["202302"],"award-info":[{"award-number":["202302"]}]},{"name":"Key Discipline Project on Chinese Pharmacology of Hunan University of Chinese Medicine, China","award":["C2022043"],"award-info":[{"award-number":["C2022043"]}]},{"name":"Key Discipline Project on Chinese Pharmacology of Hunan University of Chinese Medicine, China","award":["A2022005-4"],"award-info":[{"award-number":["A2022005-4"]}]},{"name":"Key Discipline Project on Chinese Pharmacology of Hunan University of Chinese Medicine, China","award":["202302"],"award-info":[{"award-number":["202302"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>The leaves of C. tiglium have been comprehensively researched for their structurally novel bioactive natural compounds, especially those with anti-schistosomiasis liver fibrosis activity, because ethyl acetate extract, which can be extracted from the leaves of C. tiglium, has good anti-schistosomiasis liver fibrosis effects. One new tigliane-type diterpene, 20-acetyl-13-O-(2-metyl)butyryl-phorbol (1), and nine known (2\u201310) analogues were isolated from the leaves of C. tiglium. Their structures were elucidated on the basis of spectroscopic analysis and ECD analysis. All diterpenoids had a stronger insecticidal effect on schistosomula, and compounds 2, 4, and 10 had good anti-liver-fibrosis effects. Furthermore, compared with the model group, compound 2 significantly downregulated the protein and mRNA expression of COL-I, COL-III, \u03b1-SMA, and TGF-\u03b21 on TGF-\u03b21-induced liver fibrosis in LX-2 cells. Meanwhile, compound 2 also regulated the expression of TGF-\u03b2\/Smad-pathway-related proteins. The results suggest that diterpenoids from C. tiglium may serve as potential schistosomula-killing and anti-liver-fibrosis agents in the future.<\/jats:p>","DOI":"10.3390\/molecules29020401","type":"journal-article","created":{"date-parts":[[2024,1,15]],"date-time":"2024-01-15T07:25:07Z","timestamp":1705303507000},"page":"401","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Diterpenoids with Schistosomula-Killing and Anti-Fibrosis Activities In Vitro from the Leaves of Croton tiglium"],"prefix":"10.3390","volume":"29","author":[{"given":"Li","family":"Li","sequence":"first","affiliation":[{"name":"College of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Biqing","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoxiao","family":"Zheng","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaohui","family":"Liu","sequence":"additional","affiliation":[{"name":"Hengxiu Tang Pharmaceutical Co., Ltd., Changsha 410219, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huan","family":"Zou","sequence":"additional","affiliation":[{"name":"Hengxiu Tang Pharmaceutical Co., Ltd., Changsha 410219, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Qin","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojiang","family":"Zhou","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,1,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Cheng, S.Y., Zhu, B.K., Luo, F., Lin, X.Y., Sun, C.S., You, Y.M., Yi, C., Xu, B., Wang, J.P., and Lu, Y. 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