{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T16:15:45Z","timestamp":1760890545970,"version":"build-2065373602"},"reference-count":27,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2024,1,22]],"date-time":"2024-01-22T00:00:00Z","timestamp":1705881600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Key Research and Development Program of Shaanxi","award":["2021ZDLSF03-05","2022-85","23JK0651","23JP155","23JC060","2021TD03","2021TD07","2021DOC17"],"award-info":[{"award-number":["2021ZDLSF03-05","2022-85","23JK0651","23JP155","23JC060","2021TD03","2021TD07","2021DOC17"]}]},{"name":"the Youth Innovation Team Construction Project of Shaanxi Universities","award":["2021ZDLSF03-05","2022-85","23JK0651","23JP155","23JC060","2021TD03","2021TD07","2021DOC17"],"award-info":[{"award-number":["2021ZDLSF03-05","2022-85","23JK0651","23JP155","23JC060","2021TD03","2021TD07","2021DOC17"]}]},{"name":"Shaanxi Provincial Education Department","award":["2021ZDLSF03-05","2022-85","23JK0651","23JP155","23JC060","2021TD03","2021TD07","2021DOC17"],"award-info":[{"award-number":["2021ZDLSF03-05","2022-85","23JK0651","23JP155","23JC060","2021TD03","2021TD07","2021DOC17"]}]},{"name":"the Scientific and Technological Innovation Team project of Xi\u2019an Medical University","award":["2021ZDLSF03-05","2022-85","23JK0651","23JP155","23JC060","2021TD03","2021TD07","2021DOC17"],"award-info":[{"award-number":["2021ZDLSF03-05","2022-85","23JK0651","23JP155","23JC060","2021TD03","2021TD07","2021DOC17"]}]},{"name":"Research start-up fund of Xi\u2019an Medical University","award":["2021ZDLSF03-05","2022-85","23JK0651","23JP155","23JC060","2021TD03","2021TD07","2021DOC17"],"award-info":[{"award-number":["2021ZDLSF03-05","2022-85","23JK0651","23JP155","23JC060","2021TD03","2021TD07","2021DOC17"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>DAPB, a new molecule including danshensu, borneol, and a mother nucleus of ACEI (Angiotensin-converting enzyme inhibitors), is being developed as an antihypertensive candidate compound. A rapid, accurate, and sensitive ultra-performance liquid chromatography\u2013tandem mass spectrometry (UPLC\u2013MS\/MS) method was established and validated for the determination of DAPB in rat plasma. Chromatographic separation was performed on an Agilent SB-C18 column after protein precipitation by acetonitrile with a mobile phase consisting of acetonitrile and deionized water with 0.02% formic acid and 5 mM NH4F (v\/v) at a flow rate of 0.2 mL\/min. Quantification was performed using electrospray positive ionization mass spectrometry in the multiple reaction monitoring (MRM) mode. The method was linear over the range of 2\u20131000 ng\/mL. The intra- and inter-day precision was within 12%, with accuracies less than 7%. Stability was within the acceptable limits under various storage and processing conditions. No apparent matrix effect was detected. The validated method was applied to the pre-clinical pharmacokinetic study of DAPB after oral administration of 30 mg\/kg and intravenous administration of 6 mg\/kg in rats.<\/jats:p>","DOI":"10.3390\/molecules29020541","type":"journal-article","created":{"date-parts":[[2024,1,22]],"date-time":"2024-01-22T11:36:41Z","timestamp":1705923401000},"page":"541","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["An Ultra-Performance Liquid Chromatography\u2013Tandem Mass Spectrometry (UPLC\u2013MS\/MS) Method for Qualifying DAPB in Rat Plasma and Application to Pharmacokinetic Studies"],"prefix":"10.3390","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0015-7676","authenticated-orcid":false,"given":"Bei","family":"Qin","sequence":"first","affiliation":[{"name":"Xi\u2019an Key Laboratory for Research and Development of Innovative Multi-Target Antihypertensive Drugs, Xi\u2019an Innovative Antihypertensive Drugs International Science and Technology Cooperation Base, Xi\u2019an Medical University, Xi\u2019an 710021, China"},{"name":"Institute of Drug Research, Xi\u2019an Medical University, Xi\u2019an 710021, China"},{"name":"College of Pharmacy, Xi\u2019an Medical University, Xi\u2019an 710021, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunmei","family":"Chen","sequence":"additional","affiliation":[{"name":"Xi\u2019an Key Laboratory for Research and Development of Innovative Multi-Target Antihypertensive Drugs, Xi\u2019an Innovative Antihypertensive Drugs International Science and Technology Cooperation Base, Xi\u2019an Medical University, Xi\u2019an 710021, China"},{"name":"Institute of Drug Research, Xi\u2019an Medical University, Xi\u2019an 710021, China"},{"name":"College of Pharmacy, Xi\u2019an Medical University, Xi\u2019an 710021, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4834-8084","authenticated-orcid":false,"given":"Kuan","family":"Yang","sequence":"additional","affiliation":[{"name":"Xi\u2019an Key Laboratory for Research and Development of Innovative Multi-Target Antihypertensive Drugs, Xi\u2019an Innovative Antihypertensive Drugs International Science and Technology Cooperation Base, Xi\u2019an Medical University, Xi\u2019an 710021, China"},{"name":"Institute of Drug Research, Xi\u2019an Medical University, Xi\u2019an 710021, China"},{"name":"College of Pharmacy, Xi\u2019an Medical University, Xi\u2019an 710021, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rong","family":"Wang","sequence":"additional","affiliation":[{"name":"Xi\u2019an Key Laboratory for Research and Development of Innovative Multi-Target Antihypertensive Drugs, Xi\u2019an Innovative Antihypertensive Drugs International Science and Technology Cooperation Base, Xi\u2019an Medical University, Xi\u2019an 710021, China"},{"name":"Institute of Drug Research, Xi\u2019an Medical University, Xi\u2019an 710021, China"},{"name":"College of Pharmacy, Xi\u2019an Medical University, Xi\u2019an 710021, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lili","family":"Yu","sequence":"additional","affiliation":[{"name":"Xi\u2019an Key Laboratory for Research and Development of Innovative Multi-Target Antihypertensive Drugs, Xi\u2019an Innovative Antihypertensive Drugs International Science and Technology Cooperation Base, Xi\u2019an Medical University, Xi\u2019an 710021, China"},{"name":"Institute of Drug Research, Xi\u2019an Medical University, Xi\u2019an 710021, China"},{"name":"College of Pharmacy, Xi\u2019an Medical University, Xi\u2019an 710021, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nana","family":"Wang","sequence":"additional","affiliation":[{"name":"Xi\u2019an Key Laboratory for Research and Development of Innovative Multi-Target Antihypertensive Drugs, Xi\u2019an Innovative Antihypertensive Drugs International Science and Technology Cooperation Base, Xi\u2019an Medical University, Xi\u2019an 710021, China"},{"name":"Institute of Drug Research, Xi\u2019an Medical University, Xi\u2019an 710021, China"},{"name":"College of Pharmacy, Xi\u2019an Medical University, Xi\u2019an 710021, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shaojing","family":"Liu","sequence":"additional","affiliation":[{"name":"Xi\u2019an Key Laboratory for Research and Development of Innovative Multi-Target Antihypertensive Drugs, Xi\u2019an Innovative Antihypertensive Drugs International Science and Technology Cooperation Base, Xi\u2019an Medical University, Xi\u2019an 710021, China"},{"name":"Institute of Drug Research, Xi\u2019an Medical University, Xi\u2019an 710021, China"},{"name":"College of Pharmacy, Xi\u2019an Medical University, Xi\u2019an 710021, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,1,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.numecd.2021.09.007","article-title":"Arterial hypertension\u2014Clinical trials update 2021","volume":"32","author":"Gotzinger","year":"2022","journal-title":"Nutr. 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