{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T16:11:26Z","timestamp":1787069486567,"version":"build-2736575974"},"reference-count":28,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2024,1,25]],"date-time":"2024-01-25T00:00:00Z","timestamp":1706140800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Some South American countries have ancient traditions that may pose legal problems, such as the consumption of coca leaves, as this can provide positive results for cocaine use after the analysis of biological samples. For this reason, it is necessary to find specific markers that help differentiate legal from illegal consumption, such as tropacocaine, cinnamoylcocaine, and especially hygrine and cuscohygrine. In this work, two techniques for collecting biological samples are compared: the Quantisal\u00ae Oral Fluid collection device and passive drooling. Once the samples were collected, they were subjected to solid-phase extraction for subsequent injection into GC-MS. Different validation parameters included in international guides have been studied to evaluate whether the proposed method is valid for the defined purpose, placing special emphasis on the study of the matrix effect and little value on GC-MS analyses. With respect to this parameter, an increase in the signal was found for CUS and t-CIN, but it was not significant for the rest of the substances studied. The recoveries have varied significantly depending on the way of working, being higher when working with standardized areas. After carrying out work with the oral fluid samples collected from laboratory volunteers, the method was applied to two real samples. The results obtained support the need for further research to overcome certain limitations presented by the device.<\/jats:p>","DOI":"10.3390\/molecules29030592","type":"journal-article","created":{"date-parts":[[2024,1,25]],"date-time":"2024-01-25T06:54:12Z","timestamp":1706165652000},"page":"592","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Evaluation of an Oral Fluid Collection Device and a Solid-Phase Extraction Method for the Determination of Coca Leaf Alkaloids by Gas Chromatography\u2013Mass Spectrometry"],"prefix":"10.3390","volume":"29","author":[{"given":"Pamela","family":"Cabarcos-Fern\u00e1ndez","sequence":"first","affiliation":[{"name":"Forensic Toxicology Service, Forensic Sciences Institute, Faculty of Medicine, Universidade de Santiago de Compostela, R\u00faa de San Francisco, s\/n, 15782 Santiago de Compostela, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3967-5366","authenticated-orcid":false,"given":"Ivan","family":"\u00c1lvarez-Freire","sequence":"additional","affiliation":[{"name":"Forensic Toxicology Service, Forensic Sciences Institute, Faculty of Medicine, Universidade de Santiago de Compostela, R\u00faa de San Francisco, s\/n, 15782 Santiago de Compostela, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6623-9776","authenticated-orcid":false,"given":"Nelida Cristina","family":"Rubio","sequence":"additional","affiliation":[{"name":"Forensic Toxicology Service, Forensic Sciences Institute, Faculty of Medicine, Universidade de Santiago de Compostela, R\u00faa de San Francisco, s\/n, 15782 Santiago de Compostela, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Maria","family":"Bermejo-Barrera","sequence":"additional","affiliation":[{"name":"Forensic Toxicology Service, Forensic Sciences Institute, Faculty of Medicine, Universidade de Santiago de Compostela, R\u00faa de San Francisco, s\/n, 15782 Santiago de Compostela, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3512-6434","authenticated-orcid":false,"given":"Antonio","family":"Moreda-Pi\u00f1eiro","sequence":"additional","affiliation":[{"name":"Trace Element, Spectroscopy and Speciation Group (GETEE), Institute of Materials iMATUS, Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of Chemistry, Universidade de Santiago de Compostela, Avenida das Ciencias, s\/n, 15782 Santiago de Compostela, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9734-0217","authenticated-orcid":false,"given":"Ines","family":"S\u00e1nchez-Sellero","sequence":"additional","affiliation":[{"name":"Forensic Toxicology Service, Forensic Sciences Institute, Faculty of Medicine, Universidade de Santiago de Compostela, R\u00faa de San Francisco, s\/n, 15782 Santiago de Compostela, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1069-6200","authenticated-orcid":false,"given":"Maria Jesus","family":"Tabernero-Duque","sequence":"additional","affiliation":[{"name":"Forensic Toxicology Service, Forensic Sciences Institute, Faculty of Medicine, Universidade de Santiago de Compostela, R\u00faa de San Francisco, s\/n, 15782 Santiago de Compostela, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,1,25]]},"reference":[{"key":"ref_1","unstructured":"(2023, January 09). 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