{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T04:22:20Z","timestamp":1772252540681,"version":"3.50.1"},"reference-count":18,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2024,1,31]],"date-time":"2024-01-31T00:00:00Z","timestamp":1706659200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"InterDokMed project","award":["POWR.03.02.00-00-I013\/16"],"award-info":[{"award-number":["POWR.03.02.00-00-I013\/16"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>This article discusses a new method for monitoring drug concentrations in blood samples from patients with mood disorders. The method uses solid-phase microextraction to extract analytes directly from blood samples. It has been adapted to identify the most commonly used drugs in mood disorders, including amitriptyline, citalopram, fluoxetine, paroxetine, sertraline, trazodone, duloxetine, venlafaxine, lamotrigine, quetiapine, olanzapine, and mirtazapine. The analysis is carried out using high-performance liquid chromatography coupled with mass spectroscopy. The proposed DI-SPME\/LC-MS method allows for a simple and quick screening analysis while minimizing the volume of the tested sample and solvent, in line with the principles of green analytical chemistry. The method was used to analyze 38 blood samples taken from patients with mood disorders, and drug concentrations were determined and compared with therapeutic and toxic dose ranges. This allowed for better control of the course of treatment.<\/jats:p>","DOI":"10.3390\/molecules29030676","type":"journal-article","created":{"date-parts":[[2024,2,1]],"date-time":"2024-02-01T09:43:22Z","timestamp":1706780602000},"page":"676","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Direct Immersion\u2013Solid Phase Microextraction for Therapeutic Drug Monitoring of Patients with Mood Disorders"],"prefix":"10.3390","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5988-2253","authenticated-orcid":false,"given":"Magdalena","family":"\u015awi\u0105dro-Pi\u0119to\u0144","sequence":"first","affiliation":[{"name":"Laboratory for Forensic Chemistry, Department of Analytical Chemistry, Faculty of Chemistry, Jagiellonian University, 2, Gronostajowa St., 30-387 Krak\u00f3w, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dominika","family":"Dudek","sequence":"additional","affiliation":[{"name":"Department of Adult Psychiatry, Medical College, Jagiellonian University, 21a, Miko\u0142aja Kopernika St., 31-387 Krak\u00f3w, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8908-6725","authenticated-orcid":false,"given":"Renata","family":"Wietecha-Pos\u0142uszny","sequence":"additional","affiliation":[{"name":"Laboratory for Forensic Chemistry, Department of Analytical Chemistry, Faculty of Chemistry, Jagiellonian University, 2, Gronostajowa St., 30-387 Krak\u00f3w, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,1,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"L\u00f3pez-Mu\u00f1oz, F., Shen, W.W., Docon, P., Romero, A., and \u00c1lamo, C. (2018). A history of the pharmacological treatment of bipolar disorder. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19072143"},{"key":"ref_2","first-page":"61","article-title":"Difficulties in the diagnosis of bipolar affective disorder","volume":"18","author":"Gorostowicz","year":"2018","journal-title":"J. Psychiatry Clin. Psychol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1590\/1516-4446-2020-0650","article-title":"Prevalence and correlates of major depressive disorder: A systematic review","volume":"42","author":"Dunne","year":"2020","journal-title":"Braz. J. Psychiatry"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"867840","DOI":"10.3389\/fpsyt.2022.867840","article-title":"Therapeutic Drug Monitoring of Antidepressants: An Underused but Potentially Valuable Tool in Primary Care","volume":"13","author":"Piacentino","year":"2022","journal-title":"Front. Psychiatry."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"867840","DOI":"10.3904\/kjim.2009.24.1.1","article-title":"Overview of therapeutic drug monitoring","volume":"24","author":"Kang","year":"2009","journal-title":"Korean J. Intern. Med."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"7598","DOI":"10.1016\/j.chroma.2010.10.003","article-title":"Simultaneous determination of antidepressants by non-aqueous capillary electrophoresis-time of flight mass spectrometry","volume":"1217","author":"Sasajima","year":"2010","journal-title":"J. Chromatogr. A"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Moretti, M., Freni, F., Valentini, B., Vignali, C., Groppi, A., Vison\u00e0, S.D., Osculati, A.M.M., and Morini, L. (2019). Determination of antidepressants and antipsychotics in dried blood spots (DBSs) collected from post-mortem samples and evaluation of the stability over three months. Molecules, 24.","DOI":"10.3390\/molecules24203636"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1016\/j.jchromb.2019.06.025","article-title":"Dried blood spots sampling in case samples deprived of hematocrit level information\u2014Investigation and calculation strategy","volume":"1124","author":"Majda","year":"2019","journal-title":"J. Chromatogr. B Anal. Technol. Biomed. Life Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.forsciint.2015.07.004","article-title":"Analysis of benzodiazepines and their metabolites using DBS cards and LC-MS\/MS","volume":"255","author":"Lee","year":"2015","journal-title":"Forensic Sci. Int."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1016\/S0166-526X(02)80050-2","article-title":"Chapter 13 Solid Phase Microextraction","volume":"37","author":"Pawliszyn","year":"2002","journal-title":"Compr. Anal. Chem."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Lord, H., and Bojko, B. (2012). Drug Analysis by SPME, Elsevier Inc.","DOI":"10.1016\/B978-0-12-416017-0.00010-3"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"120533","DOI":"10.1016\/j.talanta.2019.120533","article-title":"Fast and efficient analyses of the post-mortem human blood and bone marrow using DI-SPME\/LC-TOFMS method for forensic medicine purposes","volume":"209","author":"Majda","year":"2020","journal-title":"Talanta"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/j.talanta.2017.05.024","article-title":"Application of headspace and direct immersion solid-phase microextraction in the analysis of organothiophosphates related to the Chemical Weapons Convention from water and complex matrices","volume":"174","author":"Althoff","year":"2017","journal-title":"Talanta"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/S0379-0738(03)00098-7","article-title":"Simultaneous detection of some drugs of abuse in saliva samples by SPME technique","volume":"134","author":"Fucci","year":"2003","journal-title":"Forensic Sci. Int."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"7363","DOI":"10.1021\/ac9007978","article-title":"Membrane Solid-Phase Microextraction\u2014A New Concept of Sorbent Preparation","volume":"81","author":"Kloskowski","year":"2009","journal-title":"Anal. Chem."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1016\/S0021-9673(00)00192-8","article-title":"Solid-phase micro-extraction of drugs from biological matrices","volume":"885","author":"Snow","year":"2000","journal-title":"J. Chromatogr. A"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.trac.2013.04.010","article-title":"The 12 principles of green analytical chemistry and the SIGNIFICANCE mnemonic of green analytical practices","volume":"50","author":"Migaszewski","year":"2013","journal-title":"TrAC Trends Anal. Chem."},{"key":"ref_18","unstructured":"European Medicines Agency (2011). Guideline on Bioanalytical Method Validation."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/29\/3\/676\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T13:52:45Z","timestamp":1760104365000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/29\/3\/676"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1,31]]},"references-count":18,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2024,2]]}},"alternative-id":["molecules29030676"],"URL":"https:\/\/doi.org\/10.3390\/molecules29030676","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,1,31]]}}}