{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T22:11:53Z","timestamp":1779228713689,"version":"3.51.4"},"reference-count":29,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2024,2,29]],"date-time":"2024-02-29T00:00:00Z","timestamp":1709164800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Diabetes Special Fund of Hubei Provincial Key Laboratory of Cardiovascular and Cerebrovascular Diseases in Hubei University of Science and Technology","award":["2023TNB05"],"award-info":[{"award-number":["2023TNB05"]}]},{"name":"Diabetes Special Fund of Hubei Provincial Key Laboratory of Cardiovascular and Cerebrovascular Diseases in Hubei University of Science and Technology","award":["BK202120"],"award-info":[{"award-number":["BK202120"]}]},{"name":"Initial Scientific Research Fund of Ph.D. in Hubei University of Science and Technology","award":["2023TNB05"],"award-info":[{"award-number":["2023TNB05"]}]},{"name":"Initial Scientific Research Fund of Ph.D. in Hubei University of Science and Technology","award":["BK202120"],"award-info":[{"award-number":["BK202120"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Lycorine is a kind of natural active ingredient with a strong antitumor effect. In this study, folate ligand-conjugated polyethylene glycol-block-poly(l-lactide) (PEG-PLLA) nanoparticles (FA-PEG-PLLA NPs) were designed to deliver lycorine to enhance its anti-glioma activity. The successful preparation of the FA-PEG-PLLA polymer was confirmed by 1H-NMR, FT-IR, XRD, TGA, and DSC. The optimal formulation for LYC@FA-PEG-PLLA NPs was determined by response surface analysis as follows: sodium dodecyl sulfate (SDS) of 1%, carrier material of 0.03 g, organic phase volume of 3 mL, and ultrasonic power of 20%. The LYC@FA-PEG-PLLA NPs exhibited an encapsulation efficiency of 83.58% and a particle size of 49.71 nm, demonstrating good stability. Hemolysis experiments, MTT assays, and cell scratch assays revealed excellent biocompatibility of FA-PEG-PLLA and superior anti-glioma activity of LYC@FA-PEG-PLLA NPs compared to the raw drug. Additionally, cell apoptosis assays, ROS experiments, and western blot analysis demonstrated that LYC@FA-PEG-PLLA NPs contributed to cell apoptosis by inducing ROS generation and increasing the expression of NF-\u03baB inhibitory protein I\u03baB\u03b1. These results suggested that LYC@FA-PEG-PLLA NPs hold promise for glioma treatment.<\/jats:p>","DOI":"10.3390\/molecules29051081","type":"journal-article","created":{"date-parts":[[2024,2,29]],"date-time":"2024-02-29T05:59:19Z","timestamp":1709186359000},"page":"1081","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Preparation and Evaluation of Folate Modified PEG-PLLA Nanoparticles Loaded with Lycorine for Glioma Treatment"],"prefix":"10.3390","volume":"29","author":[{"given":"Jieqiong","family":"Ding","sequence":"first","affiliation":[{"name":"Department of Physiology, School of Basic Medical Sciences, Hubei University of Science and Technology, Xianning 437100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics, School of Pharmacy, Hubei University of Science and Technology, Xianning 437100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Binhua","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics, School of Pharmacy, Hubei University of Science and Technology, Xianning 437100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liqiong","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics, School of Pharmacy, Hubei University of Science and Technology, Xianning 437100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,2,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"193","DOI":"10.3233\/CBM-2011-0177","article-title":"Central nervous system","volume":"9","author":"Adamson","year":"2011","journal-title":"Cancer Biomark."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1016\/S0140-6736(18)30990-5","article-title":"Primary brain tumours in adults","volume":"392","author":"Lapointe","year":"2018","journal-title":"Lancet"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"541","DOI":"10.21037\/atm.2019.09.126","article-title":"Clinical prognostic value of isocitrate dehydrogenase mutation, O-6-methylguanine-DNA methyltransferase promoter methylation, and 1p19q co-deletion in glioma patients","volume":"7","author":"Chen","year":"2019","journal-title":"Ann. 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