{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T00:34:30Z","timestamp":1790296470453,"version":"4.1.0"},"reference-count":48,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2024,3,3]],"date-time":"2024-03-03T00:00:00Z","timestamp":1709424000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Seed Industry Innovation and Industrialization Project of Fujian Province","award":["2021FJSCZY01"],"award-info":[{"award-number":["2021FJSCZY01"]}]},{"name":"Seed Industry Innovation and Industrialization Project of Fujian Province","award":["2022-ZD-015"],"award-info":[{"award-number":["2022-ZD-015"]}]},{"name":"Seed Industry Innovation and Industrialization Project of Fujian Province","award":["2022-KF01"],"award-info":[{"award-number":["2022-KF01"]}]},{"name":"Fuzhou Science and Technology Major Project, Research and Industrialization of Functional Products of Kelp Fermentation Deodorization Coupling Peptide","award":["2021FJSCZY01"],"award-info":[{"award-number":["2021FJSCZY01"]}]},{"name":"Fuzhou Science and Technology Major Project, Research and Industrialization of Functional Products of Kelp Fermentation Deodorization Coupling Peptide","award":["2022-ZD-015"],"award-info":[{"award-number":["2022-ZD-015"]}]},{"name":"Fuzhou Science and Technology Major Project, Research and Industrialization of Functional Products of Kelp Fermentation Deodorization Coupling Peptide","award":["2022-KF01"],"award-info":[{"award-number":["2022-KF01"]}]},{"name":"Fujian Province Key Laboratory for the Development of Bioactive Material from Marine Algae, the construction of kelp odor evaluation model and the key technology of deodorization","award":["2021FJSCZY01"],"award-info":[{"award-number":["2021FJSCZY01"]}]},{"name":"Fujian Province Key Laboratory for the Development of Bioactive Material from Marine Algae, the construction of kelp odor evaluation model and the key technology of deodorization","award":["2022-ZD-015"],"award-info":[{"award-number":["2022-ZD-015"]}]},{"name":"Fujian Province Key Laboratory for the Development of Bioactive Material from Marine Algae, the construction of kelp odor evaluation model and the key technology of deodorization","award":["2022-KF01"],"award-info":[{"award-number":["2022-KF01"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Food-derived angiotensin-I-converting enzyme (ACE)-inhibitory peptides have gained attention for their potent and safe treatment of hypertensive disorders. However, there are some limitations of conventional methods for preparing ACE-inhibitory peptides. In this study, in silico hydrolysis, the quantitative structure\u2013activity relationship (QSAR) model, LC-MS\/MS, inhibition kinetics, and molecular docking were used to investigate the stability, hydrolyzability, in vitro activity, and inhibition mechanism of bioactive peptides during the actual hydrolysis process. Six novel ACE-inhibitory peptides were screened from the Larimichthys crocea protein (LCP) and had low IC50 values (from 0.63 \u00b1 0.09 \u00b5M to 10.26 \u00b1 0.21 \u00b5M), which were close to the results of the QSAR model. After in vitro gastrointestinal simulated digestion activity of IPYADFK, FYEPFM and NWPWMK were found to remain almost unchanged, whereas LYDHLGK, INEMLDTK, and IHFGTTGK were affected by gastrointestinal digestion. Meanwhile, the inhibition kinetics and molecular docking results were consistent in that ACE-inhibitory peptides of different inhibition forms could effectively bind to the active or non-central active centers of ACE through hydrogen bonding. Our proposed method has better reproducibility, accuracy, and higher directivity than previous methods. This study can provide new approaches for the deep processing, identification, and preparation of Larimichthys crocea.<\/jats:p>","DOI":"10.3390\/molecules29051134","type":"journal-article","created":{"date-parts":[[2024,3,4]],"date-time":"2024-03-04T04:36:21Z","timestamp":1709526981000},"page":"1134","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Biodirected Screening and Preparation of Larimichthys crocea Angiotensin-I-Converting Enzyme-Inhibitory Peptides by a Combined In Vitro and In Silico Approach"],"prefix":"10.3390","volume":"29","author":[{"given":"Zhizhi","family":"Yang","sequence":"first","affiliation":[{"name":"College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changrong","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baote","family":"Huang","sequence":"additional","affiliation":[{"name":"College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yihui","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiyu","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Cultivation and High-Value Utilization of Marine Organisms in Fujian Province, Fisheries Research Institute of Fujian, National Research and Development Center for Marine Fish Processing (Xiamen), Xiamen 361013, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongbin","family":"Chen","sequence":"additional","affiliation":[{"name":"Fujian Province Key Laboratory for the Development of Bioactive Material from Marine Algae, Quanzhou Normal University, Quanzhou 362000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jicheng","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,3,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1016\/j.tifs.2023.07.008","article-title":"Recent Advances in Understanding Dietary Polyphenols Protecting against Hypertension","volume":"138","author":"Gai","year":"2023","journal-title":"Trends Food Sci. 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