{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:55:07Z","timestamp":1760144107241,"version":"build-2065373602"},"reference-count":72,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2024,3,23]],"date-time":"2024-03-23T00:00:00Z","timestamp":1711152000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>A fungal isolate Aspergillus terreus PDB-B (accession number: MT774567.1), which could tolerate up to 500 mg\/L of cypermethrin, was isolated from the lake sediments of Kulamangalam tropical lake, Madurai, and identified by internal transcribed spacer (ITS) sequencing followed by phylogenetic analysis. The biotransformation potential of the strain was compared with five other strains (A, J, UN2, M1 and SM108) as a consortium, which were tentatively identified as Aspergillus glaucus, Aspergillus niger, Aspergillus flavus, Aspergillus terreus, and Aspergillus flavus, respectively. Batch culture and soil microcosm studies were conducted to explore biotransformation using plate-based enzymatic screening and GC-MS. A mycotransformation pathway was predicted based on a comparative analysis of the transformation products (TPs) obtained. The cytotoxicity assay revealed that the presence of (3-methylphenyl) methanol and isopropyl ether could be relevant to the high rate of lethality.<\/jats:p>","DOI":"10.3390\/molecules29071446","type":"journal-article","created":{"date-parts":[[2024,3,25]],"date-time":"2024-03-25T12:28:06Z","timestamp":1711369686000},"page":"1446","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Mycotransformation of Commercial Grade Cypermethrin Dispersion by Aspergillus terreus PDB-B Strain Isolated from Lake Sediments of Kulamangalam, Madurai"],"prefix":"10.3390","volume":"29","author":[{"given":"Priyadharshini","family":"Kannan","sequence":"first","affiliation":[{"name":"Department of Microbiology, The American College, Madurai 625002, Tamil Nadu, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hidayah","family":"Baskaran","sequence":"additional","affiliation":[{"name":"Department of Microbiology, The American College, Madurai 625002, Tamil Nadu, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0610-0371","authenticated-orcid":false,"given":"Jemima Balaselvi","family":"Juliana Selvaraj","sequence":"additional","affiliation":[{"name":"Department of Chemistry, The American College, Madurai 625002, Tamil Nadu, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1755-3002","authenticated-orcid":false,"given":"Agnieszka","family":"Saeid","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, Politechnika Wroclawska, 50-370 Wroclaw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9595-7096","authenticated-orcid":false,"given":"Jennifer Michellin","family":"Kiruba Nester","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, Politechnika Wroclawska, 50-370 Wroclaw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,3,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"916","DOI":"10.1126\/science.aat3466","article-title":"Increase in crop losses to insect pests in a warming climate","volume":"361","author":"Deutsch","year":"2018","journal-title":"Science"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1038\/s41561-021-00712-5","article-title":"Risk of pesticide pollution at the global scale","volume":"14","author":"Tang","year":"2021","journal-title":"Nat. Geosci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/j.ejar.2020.08.007","article-title":"Pesticides pollution: Classifications, human health impact, extraction and treatment techniques","volume":"46","author":"Hassaan","year":"2020","journal-title":"Egypt. J. Aquat. Res."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41598-018-31082-5","article-title":"Presence of esterase and laccase in Bacillus subtilis facilitates biodegradation and detoxification of cypermethrin","volume":"8","author":"Gangola","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"863","DOI":"10.1016\/j.jenvman.2017.11.076","article-title":"Cypermethrin induced toxicities in fish and adverse health outcomes: Its prevention and control measure adaptation","volume":"206","author":"Ullah","year":"2018","journal-title":"J. Environ. Manag."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Farag, M.R., Alagawany, M., Bilal, R.M., Gewida, A.G., Dhama, K., Abdel-Latif, H.M., Amer, M.S., Rivero-Perez, N., Zaragoza-Bastida, A., and Binnaser, Y.S. (2021). An overview on the potential hazards of pyrethroid insecticides in fish, with special emphasis on cypermethrin toxicity. Animals, 11.","DOI":"10.3390\/ani11071880"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"125507","DOI":"10.1016\/j.chemosphere.2019.125507","article-title":"Esterase is a powerful tool for the biodegradation of pyrethroid insecticides","volume":"244","author":"Bhatt","year":"2020","journal-title":"Chemosphere"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1007\/s00253-018-9451-1","article-title":"Roles of saprotrophic fungi in biodegradation or transformation of organic and inorganic pollutants in co-contaminated sites","volume":"103","author":"Ceci","year":"2019","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Das, S. (2014). Microbial Biodegradation and Bioremediation, Elsevier.","DOI":"10.1016\/B978-0-12-800021-2.00001-7"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1949","DOI":"10.1007\/s13213-015-1033-1","article-title":"In vitro study of mycoremediation of cypermethrin-contaminated soils in different regions of Punjab","volume":"65","author":"Kaur","year":"2015","journal-title":"Ann. Microbiol."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Hu, K., Deng, W., Zhu, Y., Yao, K., Li, J., Liu, A., Ao, X., Zou, L., Zhou, K., and He, L. (2019). Simultaneous degradation of \u03b2-cypermethrin and 3-phenoxybenzoic acid by Eurotium cristatum ET1, a novel \u201cgolden flower fungus\u201d strain isolated from Fu Brick Tea. MicrobiologyOpen, 8.","DOI":"10.1002\/mbo3.776"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Aguila-Torres, P., Maldonado, J., Gaete, A., Figueroa, J., Gonz\u00e1lez, A., Miranda, R., Gonz\u00e1lez-Stegmaier, R., Martin, C., and Gonz\u00e1lez, M. (2020). Biochemical and genomic characterization of the cypermethrin-degrading and biosurfactant-producing bacterial strains isolated from marine sediments of the Chilean Northern Patagonia. Mar. Drugs, 18.","DOI":"10.3390\/md18050252"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"129000","DOI":"10.1016\/j.foodchem.2021.129000","article-title":"Screening of lactic acid bacteria for their capacity to bind cypermethrin in vitro and the binding characteristics and its application","volume":"347","author":"Zhang","year":"2021","journal-title":"Food Chem."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"130152","DOI":"10.1016\/j.chemosphere.2021.130152","article-title":"Biodegradation competence of Streptomyces toxytricini D2 isolated from leaves surface of the hybrid cotton crop against \u03b2 cypermethrin","volume":"276","author":"Whangchai","year":"2021","journal-title":"Chemosphere"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"113137","DOI":"10.1016\/j.envres.2022.113137","article-title":"Indigenous bacterial consortium-mediated cypermethrin degradation in the presence of organic amendments and Zea mays plants","volume":"212","author":"Bhatt","year":"2022","journal-title":"Environ. Res."},{"key":"ref_16","first-page":"1","article-title":"Biodegradation \u03b1-endosulfan and \u03b1-cypermethrin by Acinetobacter schindleri B7 isolated from the microflora of grasshopper (Poecilimon tauricola)","volume":"204","author":"Algur","year":"2022","journal-title":"Arch. Microbiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"132961","DOI":"10.1016\/j.cej.2021.132961","article-title":"Mechanism of \u03b2-cypermethrin metabolism by Bacillus cereus GW-01","volume":"430","author":"Zhao","year":"2022","journal-title":"Chem. Eng. J."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"127101","DOI":"10.1016\/j.jhazmat.2021.127101","article-title":"Characterization of a novel beta-cypermethrin-degrading strain of Lactobacillus pentosus 3\u201327 and its effects on bioremediation and the bacterial community of contaminated alfalfa silage","volume":"423","author":"Liu","year":"2022","journal-title":"J. Hazard. Mater."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s00284-021-02744-x","article-title":"Analysis of Biodegradation of the Synthetic Pyrethroid Cypermethrin by Beauveria bassiana","volume":"79","author":"Muthabathula","year":"2022","journal-title":"Curr. Microbiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"944","DOI":"10.1080\/03650340.2020.1864339","article-title":"The biotransformation potential of Bacillus cereus on \u03b2-cypermethrin to protect the earthworm (Perionyx excavatus) on insecticide-contaminated soil","volume":"68","author":"Narayanan","year":"2022","journal-title":"Arch. Agron. Soil Sci."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Saied, E., Fouda, A., Alemam, A.M., Sultan, M.H., Barghoth, M.G., Radwan, A.A., Desouky, S.G., Azab, I.H.E., Nahhas, N.E., and Hassan, S.E.D. (2021). Evaluate the Toxicity of Pyrethroid Insecticide Cypermethrin before and after Biodegradation by Lysinibacillus cresolivuorans Strain HIS7. Plants, 10.","DOI":"10.3390\/plants10091903"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"114073","DOI":"10.1016\/j.envpol.2020.114073","article-title":"Effective mycoremediation coupled with bioaugmentation studies: An advanced study on newly isolated Aspergillus sp. in Type-II pyrethroid-contaminated soil","volume":"261","author":"Kaur","year":"2020","journal-title":"Environ. Pollut."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Bhatt, P., Huang, Y., Zhang, W., Sharma, A., and Chen, S. (2020). Enhanced cypermethrin degradation kinetics and metabolic pathway in Bacillus thuringiensis strain SG4. Microorganisms, 8.","DOI":"10.3390\/microorganisms8020223"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1071\/SR9850661","article-title":"Urease and dehydrogenase activity of alkali and reclaimed soils","volume":"23","author":"Rao","year":"1985","journal-title":"Soil Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1007\/BF00483972","article-title":"The regulation of urease activity in Aspergillus nidulans","volume":"20","author":"Mackay","year":"1982","journal-title":"Biochem. Genet."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1007\/s13205-015-0328-z","article-title":"Catalytic properties, functional attributes and industrial applications of \u03b2-glucosidases","volume":"6","author":"Singh","year":"2016","journal-title":"3 Biotech"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1631\/jzus.B1500317","article-title":"Characterization of \u03b2-glucosidase from Aspergillus terreus and its application in the hydrolysis of soybean isoflavones","volume":"17","author":"Yan","year":"2016","journal-title":"J. Zhejiang Univ. Sci. B"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Junior, A.B., Borges, D.G., Tardioli, P.W., and Farinas, C.S. (2014). Characterization of \u03b2-glucosidase produced by Aspergillus niger under solid-state fermentation and partially purified using MANAE-Agarose. Biotechnol. Res. Int., 2014.","DOI":"10.1155\/2014\/317092"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"652","DOI":"10.1080\/10942912.2015.1023398","article-title":"Purification and Characterization of \u03b2-Glucosidase from Aspergillus niger","volume":"19","author":"Narasimha","year":"2016","journal-title":"Int. J. Food Prop."},{"key":"ref_30","first-page":"19607","article-title":"Purification and characterization of b-1, 4-glucosidase from Aspergillus glaucus","volume":"10","author":"Ma","year":"2011","journal-title":"Afr. J. Biotechnol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1099\/00221287-140-4-779","article-title":"Purification and characterization of a ferulic acid esterase (FAE-III) from Aspergillus niger: Specificity for the phenolic moiety and binding to microcrystalline cellulose","volume":"140","author":"Faulds","year":"1994","journal-title":"Microbiology"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2011","DOI":"10.1099\/00221287-144-8-2011","article-title":"Hairy plant polysaccharides: A close shave with microbial esterases","volume":"144","author":"Williamson","year":"1998","journal-title":"Microbiology"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"4638","DOI":"10.1128\/aem.63.12.4638-4644.1997","article-title":"The faeA genes from Aspergillus niger and Aspergillus tubingensis encode ferulic acid esterases involved in degradation of complex cell wall polysaccharides","volume":"63","author":"Michelsen","year":"1997","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Liu, Y., Xu, H., Yan, Q., Yang, S., Duan, X., and Jiang, Z. (2013). Biochemical characterization of a first fungal esterase from Rhizomucor miehei showing high efficiency of ester synthesis. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0077856"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1545","DOI":"10.1016\/j.soilbio.2004.07.019","article-title":"Ligninolytic fungi in bioremediation: Extracellular enzyme production and degradation rate","volume":"36","author":"Cajthaml","year":"2004","journal-title":"Soil Biol. Biochem."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1007\/s10562-019-03096-9","article-title":"Ligninolytic enzymes mediated ligninolysis: An untapped biocatalytic potential to deconstruct lignocellulosic molecules in a sustainable manner","volume":"150","author":"Bilal","year":"2020","journal-title":"Catal. Lett."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1111\/j.1574-6968.2001.tb10676.x","article-title":"Molecular characterization of a blue-copper laccase, TILA, of Aspergillus nidulans","volume":"199","author":"Scherer","year":"2001","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_38","first-page":"423","article-title":"Absence of laccase from yellow-spored mutants of Aspergillus nidulans","volume":"70","author":"Clutterbuck","year":"1972","journal-title":"Microbiology"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.enzmictec.2009.07.014","article-title":"Production of laccase, manganese peroxidase and lignin peroxidase by Brazilian marine-derived fungi","volume":"46","author":"Durrant","year":"2010","journal-title":"Enzym. Microb. Technol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s13205-017-0820-8","article-title":"Biomineralisation of fipronil and its major metabolite, fipronil sulfone, by Aspergillus glaucus strain AJAG1 with enzymes studies and bioformulation","volume":"7","author":"Gajendiran","year":"2017","journal-title":"3 Biotech"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"e01628-18","DOI":"10.1128\/AEM.01628-18","article-title":"Versatile fungal polyphenol oxidase with chlorophenol bioremediation potential: Characterization and protein engineering","volume":"84","author":"Nikolaivits","year":"2018","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1002\/ps.2780080402","article-title":"Degradation of the pyrethroid cypermethrin NRDC 149 (\u00b1)-\u03b1-cyano-3-phenoxybenzyl (\u00b1)-cis, trans-3-(2, 2-dichlorovinyl)-2, 2-dimethylcyclopropanecarboxylate and the respective cis-(NRDC 160) and trans-(NRDC 159) isomers in soils","volume":"8","author":"Roberts","year":"1977","journal-title":"Pestic. Sci."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1021\/jf60222a059","article-title":"Metabolism of trans-and cis-permethrin, trans-and cis-cypermethrin, and decamethrin by microsomal enzymes","volume":"27","author":"Shono","year":"1979","journal-title":"J. Agric. Food Chem."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1023\/B:BIOD.0000009973.53531.96","article-title":"Bioremediation of BTEX hydrocarbons: Effect of soil inoculation with the toluene-growing fungus Cladophialophora sp. strain T1","volume":"15","author":"Ballerstedt","year":"2004","journal-title":"Biodegradation"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"247","DOI":"10.14723\/tmrsj.41.247","article-title":"Biotransformation of acetophenone to 1-phenylethanol by fungi","volume":"41","author":"Nagaki","year":"2016","journal-title":"Trans. Mater. Res. Soc. Jpn."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1139\/v87-087","article-title":"Side chain hydroxylation of aromatic compounds by fungi.: 1. Products and stereochemistry","volume":"65","author":"Holland","year":"1987","journal-title":"Can. J. Chem."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/j.cbpa.2014.01.015","article-title":"Oxidations catalyzed by fungal peroxygenases","volume":"19","author":"Hofrichter","year":"2014","journal-title":"Curr. Opin. Chem. Biol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1016\/j.tetasy.2009.02.009","article-title":"Stereoselective biotransformations using fungi as biocatalysts","volume":"20","author":"Borges","year":"2009","journal-title":"Tetrahedron Asymmetry"},{"key":"ref_49","first-page":"543","article-title":"Production of (R)-1-phenylethanols through bioreduction of acetophenones by a new fungus isolate Trichothecium roseum","volume":"22","author":"Zilbeyaz","year":"2010","journal-title":"Chirality Pharmacol. Biol. Chem. Conseq. Mol. Asymmetry"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"958","DOI":"10.1271\/bbb.56.958","article-title":"Asymmetric reduction of acetophenone by Aspergillus species and their possible contribution to katsuobushi flavor","volume":"56","author":"Doi","year":"1992","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/j.molcatb.2004.08.006","article-title":"Evaluation of acetophenone monooxygenase and alcohol dehydrogenase activities in different fungal strains by biotransformation of acetophenone derivatives","volume":"31","author":"Andrade","year":"2004","journal-title":"J. Mol. Catal. B Enzym."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1039\/b312469j","article-title":"Enantioselective hydrolysis of various substituted styrene oxides with Aspergillus niger CGMCC 0496","volume":"2","author":"Jin","year":"2004","journal-title":"Org. Biomol. Chem."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"8110","DOI":"10.1016\/j.biortech.2011.06.055","article-title":"Isolation and characterization of a fungus able to degrade pyrethroids and 3-phenoxybenzaldehyde","volume":"102","author":"Chen","year":"2011","journal-title":"Bioresour. Technol."},{"key":"ref_54","first-page":"1","article-title":"Pathway and kinetics of cyhalothrin biodegradation by Bacillus thuringiensis strain ZS-19","volume":"5","author":"Chen","year":"2015","journal-title":"Sci. Rep."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1220","DOI":"10.1021\/jf0349580","article-title":"Biodegradation of \u03b2-cyfluthrin by fungi","volume":"52","author":"Saikia","year":"2004","journal-title":"J. Agric. Food Chem."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"3423","DOI":"10.1016\/j.biortech.2009.12.083","article-title":"Biodegradation of beta-cypermethrin by two Serratia spp. with different cell surface hydrophobicity","volume":"101","author":"Zhang","year":"2010","journal-title":"Bioresour. Technol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1139\/cjm-2012-0580","article-title":"Biodegradation of cypermethrin by a novel Catellibacterium sp. strain CC-5 isolated from contaminated soil","volume":"59","author":"Zhao","year":"2013","journal-title":"Can. J. Microbiol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1007\/s10653-007-9108-y","article-title":"Persistence and dissipation of synthetic pyrethroid pesticides in red soils from the Yangtze River delta area","volume":"30","author":"Gu","year":"2008","journal-title":"Environ. Geochem. Health"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.jhazmat.2015.03.062","article-title":"Microbial degradation of alpha-cypermethrin in soil by compound-specific stable isotope analysis","volume":"295","author":"Xu","year":"2015","journal-title":"J. Hazard. Mater."},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Margesin, R., and Schinner, F. (2005). Manual for Soil Analysis-Monitoring and Assessing Soil Bioremediation, Springer Science & Business Media.","DOI":"10.1007\/3-540-28904-6"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"177","DOI":"10.5598\/imafungus.2011.02.02.09","article-title":"Molecular techniques for pathogen identification and fungus detection in the environment","volume":"2","author":"Tsui","year":"2011","journal-title":"IMA Fungus"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"W5","DOI":"10.1093\/nar\/gkn201","article-title":"NCBI BLAST: A better web interface","volume":"36","author":"Johnson","year":"2008","journal-title":"Nucleic Acids Res."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"W465","DOI":"10.1093\/nar\/gkn180","article-title":"Phylogeny. fr: Robust phylogenetic analysis for the non-specialist","volume":"36","author":"Dereeper","year":"2008","journal-title":"Nucleic Acids Res."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"W260","DOI":"10.1093\/nar\/gkz303","article-title":"NGPhylogeny. fr: New generation phylogenetic services for non-specialists","volume":"47","author":"Lemoine","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"W293","DOI":"10.1093\/nar\/gkab301","article-title":"Interactive Tree Of Life (iTOL) v5: An online tool for phylogenetic tree display and annotation","volume":"49","author":"Letunic","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"ref_66","unstructured":"Sangeetha, J., and Thangadurai, D. (2013). Laboratory Protocols in Fungal Biology, Springer."},{"key":"ref_67","first-page":"17","article-title":"Qualitative methods for the determination of lignocellulolytic enzyme production by tropical fungi","volume":"2","author":"Pointing","year":"1999","journal-title":"Fungal Divers."},{"key":"ref_68","first-page":"1240","article-title":"An evaluation of optical density to estimate fungal spore concentrations in water suspensions","volume":"1","author":"Morris","year":"1978","journal-title":"Strain"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"1378","DOI":"10.1080\/03067319.2011.581365","article-title":"Analysis of cypermethrin residues and its main degradation products in soil and formulation samples by gas chromatography-electron impact-mass spectrometry in the selective ion monitoring mode","volume":"92","author":"Garoiaz","year":"2012","journal-title":"Int. J. Environ. Anal. Chem."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"D502","DOI":"10.1093\/nar\/gkv1229","article-title":"enviPath\u2013The environmental contaminant biotransformation pathway resource","volume":"44","author":"Wicker","year":"2016","journal-title":"Nucleic Acids Res."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"1322","DOI":"10.1039\/D3EM00161J","article-title":"Combining predictive and analytical methods to elucidate pharmaceutical biotransformation in activated sludge","volume":"25","author":"Trostel","year":"2023","journal-title":"Environ. Sci. Process. Impacts"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"23","DOI":"10.3109\/15376516.2011.583297","article-title":"An improved brine shrimp larvae lethality microwell test method","volume":"22","author":"Zhang","year":"2012","journal-title":"Toxicol. Mech. Methods"}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/29\/7\/1446\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T14:17:35Z","timestamp":1760105855000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/29\/7\/1446"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,3,23]]},"references-count":72,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2024,4]]}},"alternative-id":["molecules29071446"],"URL":"https:\/\/doi.org\/10.3390\/molecules29071446","relation":{},"ISSN":["1420-3049"],"issn-type":[{"type":"electronic","value":"1420-3049"}],"subject":[],"published":{"date-parts":[[2024,3,23]]}}}